################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BciVI BclI BfaI BfrI BglI BlnI BsaBI BseAI BsgI BsiEI BsiWI BsmFI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV FbaI FseI FspI HpaI KpnI MaeI MamI MluI MroI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI TfiI Tsp45I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Alw44I 207 GTGCAC G^TGCAC AlwNI 999 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 207 GTGCAC G^TGCAC AvaII 616 GGTCC G^G[AT]CC BalI 758 TGGCCA TGG^CCA BbsI 1062 GAAGAC GAAGAC(2/6) BbvCI 1268 CCTCAGC CCTCAGC(-5/-2) BcgI 143 GCATCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 555 AGATCT A^GATCT BlpI 1026 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1026 GCTTAGC GC^T[ACGT]AGC BpuAI 1062 GAAGAC GAAGAC(2/6) BsaAI 595 TACGTG [CT]AC^GT[GA] BsaWI 563 ACCGGA [AT]^CCGG[AT] BspHI 798 TCATGA T^CATGA BsrDI 539 GCAATG GCAATG(2/0) BsrFI 119 GCCGGC [GA]^CCGG[CT] BssHII 20 GCGCGC G^CGCGC BssSI 187 CTCGTG CACGAG(-5/-1) BstAPI 43 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1153 GGTAACC G^GT[ACGT]ACC BstPI 1153 GGTAACC G^GT[ACGT]ACC BstYI 555 AGATCT [GA]^GATC[CT] CelII 1026 GCTTAGC GC^T[ACGT]AGC Cfr10I 119 GCCGGC [GA]^CCGG[CT] EcoO65I 1153 GGTAACC G^GT[ACGT]ACC EcoT22I 337 ATGCAT ATGCA^T HincII 427 GTCAAC GT[CT]^[GA]AC HindII 427 GTCAAC GT[CT]^[GA]AC KspI 98 CCGCGG CCGC^GG MaeII 596 ACGT A^CGT MflI 555 AGATCT [GA]^GATC[CT] MluNI 758 TGGCCA TGG^CCA MscI 758 TGGCCA TGG^CCA MspA1I 98 CCGCGG C[AC]G^C[GT]G NaeI 119 GCCGGC GCC^GGC NcoI 741 CCATGG C^CATGG NdeI 770 CATATG CA^TATG NgoMIV 119 GCCGGC G^CCGGC NsiI 337 ATGCAT ATGCA^T PflMI 834 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 420 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 798 TCATGA T^CATGA SacI 259 GAGCTC GAGCT^C SacII 98 CCGCGG CCGC^GG SgrAI 118 CGCCGGCG C[GA]^CCGG[CT]G SwaI 318 ATTTAAAT ATTT^AAAT Van91I 834 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1172 CCACCATCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 555 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 316 GAATTT [GA]^AATT[CT] 1422 AAATTT AlwI 435 GATCC GGATC(4/5) 657 GGATC ApoI 316 GAATTT [GA]^AATT[CT] 1422 AAATTT BanII 85 GAGCCC G[GA]GC[CT]^C 259 GAGCTC BsmBI 497 CGTCTC CGTCTC(1/5) 778 GAGACG BsmI 1167 GAATGC GAATGC(1/-1) 449 GCATTC BstXI 297 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1175 CCATCTCCGTGG DraI 319 TTTAAA TTT^AAA 1419 TTTAAA EcoT14I 686 CCAAGG C^C[AT][AT]GG 741 CCATGG HindIII 670 AAGCTT A^AGCTT 837 AAGCTT HinfI 1056 GACTC G^A[ACGT]TC 1348 GAGTC MaeIII 1154 GTAAC ^GT[ACGT]AC 1204 GTTAC MfeI 422 CAATTG C^AATTG 1160 CAATTG MslI 340 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1176 CATCTCCGTG MunI 422 CAATTG C^AATTG 1160 CAATTG PleI 1056 GACTC GAGTC(4/5) 1348 GAGTC PstI 127 CTGCAG CTGCA^G 202 CTGCAG SfaNI 975 GATGC GCATC(5/9) 143 GCATC SfcI 127 CTGCAG C^T[GA][CT]AG 202 CTGCAG SmaI 88 CCCGGG CCC^GGG 845 CCCGGG SmlI 514 CTCAAG C^T[CT][GA]AG 1105 CTTGAG StyI 686 CCAAGG C^C[AT][AT]GG 741 CCATGG TspRI 44 CACTG CA[GC]TG[ACGT][ACGT]^ 205 CAGTG XmaCI 88 CCCGGG C^CCGGG 845 CCCGGG XmaI 88 CCCGGG C^CCGGG 845 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaI BanI BbeI BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI Ksp632I MboI MboII MnlI MseI MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfoI TaqI Tru9I TseI Tsp509I TthHB8I