(Linear) (Six-base) MAPSORT of: KIEE4459.seq Check: 1539 from: 1 to: 1428 KIEE4459.orf With 182 enzymes: SgfI * March 22, 2012 18:05 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1070 1081 1428 Size: 1070 11 347 Fragments arranged by size: 1070 347 11 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 40 1428 Size: 40 1388 AlwNI CAG_nnn'CTG Cuts at: 0 1004 1428 Size: 1004 424 ApaLI G'TGCA_C Cuts at: 0 207 1428 Size: 207 1221 ApoI r'AATT_y Cuts at: 0 316 1422 1428 Size: 316 1106 6 Fragments arranged by size: 1106 316 6 AvaI C'yCGr_G Cuts at: 0 88 169 845 1428 Size: 88 81 676 583 Fragments arranged by size: 676 583 88 81 BanI G'GyrC_C Cuts at: 0 70 93 116 1428 Size: 70 23 23 1312 Fragments arranged by size: 1312 70 23 23 BanII G_rGCy'C Cuts at: 0 89 263 1428 Size: 89 174 1165 Fragments arranged by size: 1165 174 89 BbeI G_GCGC'C Cuts at: 0 74 97 120 1428 Size: 74 23 23 1308 Fragments arranged by size: 1308 74 23 23 BbsI GAAGACnn'nnnn_ Cuts at: 0 1069 1428 Size: 1069 359 BbvCI CC'TCA_GC Cuts at: 0 1269 1428 Size: 1269 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 132 166 1428 Size: 132 34 1262 Fragments arranged by size: 1262 132 34 BfrBI ATG'CAT Cuts at: 0 339 1428 Size: 339 1089 BglII A'GATC_T Cuts at: 0 555 1428 Size: 555 873 BlpI GC'TnA_GC Cuts at: 0 1027 1428 Size: 1027 401 Bme1580I G_kGCm'C Cuts at: 0 45 211 1428 Size: 45 166 1217 Fragments arranged by size: 1217 166 45 BmrI ACTGGGnnnn_n' Cuts at: 0 378 1080 1428 Size: 378 702 348 Fragments arranged by size: 702 378 348 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 385 1428 Size: 385 1043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 989 1251 1259 1366 1428 Size: 989 262 8 107 62 Fragments arranged by size: 989 262 107 62 8 Bpu10I CC'TnA_GC Cuts at: 0 994 1269 1428 Size: 994 275 159 Fragments arranged by size: 994 275 159 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 499 1126 1428 Size: 499 627 302 Fragments arranged by size: 627 499 302 BsaI GGTCTCn'nnnn_ Cuts at: 0 676 743 1136 1428 Size: 676 67 393 292 Fragments arranged by size: 676 393 292 67 BsaAI yAC'GTr Cuts at: 0 597 1428 Size: 597 831 BsaHI Gr'CG_yC Cuts at: 0 71 94 117 293 824 1428 Size: 71 23 23 176 531 604 Fragments arranged by size: 604 531 176 71 23 23 BsaWI w'CCGG_w Cuts at: 0 563 1428 Size: 563 865 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 130 160 740 770 777 796 807 826 Size: 130 30 580 30 7 19 11 19 Cuts at: 826 1072 1102 1428 Size: 246 30 326 Fragments arranged by size: 580 326 246 130 30 30 30 19 19 11 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 761 801 1136 1387 1428 Size: 761 40 335 251 41 Fragments arranged by size: 761 335 251 41 40 BseYI C'CCAG_C Cuts at: 0 1327 1428 Size: 1327 101 BsiHKAI G_wGCw'C Cuts at: 0 211 263 798 1428 Size: 211 52 535 630 Fragments arranged by size: 630 535 211 52 BsmI GAATG_Cn' Cuts at: 0 449 1173 1428 Size: 449 724 255 Fragments arranged by size: 724 449 255 BsmBI CGTCTCn'nnnn_ Cuts at: 0 503 772 1428 Size: 503 269 656 Fragments arranged by size: 656 503 269 Bsp1286I G_dGCh'C Cuts at: 0 45 89 211 263 798 1428 Size: 45 44 122 52 535 630 Fragments arranged by size: 630 535 122 52 45 44 BspHI T'CATG_A Cuts at: 0 798 1428 Size: 798 630 BsrDI GCAATG_nn' Cuts at: 0 546 1428 Size: 546 882 BsrFI r'CCGG_y Cuts at: 0 119 1428 Size: 119 1309 BssHII G'CGCG_C Cuts at: 0 20 1428 Size: 20 1408 BssSI C'ACGA_G Cuts at: 0 187 1428 Size: 187 1241 BstAPI GCAn_nnn'nTGC Cuts at: 0 49 1428 Size: 49 1379 BstEII G'GTnAC_C Cuts at: 0 1153 1428 Size: 1153 275 BstXI CCAn_nnnn'nTGG Cuts at: 0 304 1182 1428 Size: 304 878 246 Fragments arranged by size: 878 304 246 BstYI r'GATC_y Cuts at: 0 555 1428 Size: 555 873 BtgI C'CryG_G Cuts at: 0 98 741 1019 1181 1428 Size: 98 643 278 162 247 Fragments arranged by size: 643 278 247 162 98 BtsI GCAGTG_nn' Cuts at: 0 211 1428 Size: 211 1217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1283 1318 1428 Size: 1283 35 110 Fragments arranged by size: 1283 110 35 DraI TTT'AAA Cuts at: 0 321 1421 1428 Size: 321 1100 7 Fragments arranged by size: 1100 321 7 EaeI y'GGCC_r Cuts at: 0 2 758 869 889 1428 Size: 2 756 111 20 539 Fragments arranged by size: 756 539 111 20 2 EarI CTCTTCn'nnn_ Cuts at: 0 861 1046 1364 1428 Size: 861 185 318 64 Fragments arranged by size: 861 318 185 64 EcoICRI GAG'CTC Cuts at: 0 261 1428 Size: 261 1167 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 989 1070 1081 1251 1259 1366 1428 Size: 989 81 11 170 8 107 62 Fragments arranged by size: 989 170 107 81 62 11 8 HaeII r_GCGC'y Cuts at: 0 74 97 120 187 1428 Size: 74 23 23 67 1241 Fragments arranged by size: 1241 74 67 23 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 130 162 1192 1224 1428 Size: 130 32 1030 32 204 Fragments arranged by size: 1030 204 130 32 32 HincII GTy'rAC Cuts at: 0 429 1428 Size: 429 999 HindIII A'AGCT_T Cuts at: 0 670 837 1428 Size: 670 167 591 Fragments arranged by size: 670 591 167 KasI G'GCGC_C Cuts at: 0 70 93 116 1428 Size: 70 23 23 1312 Fragments arranged by size: 1312 70 23 23 MfeI C'AATT_G Cuts at: 0 422 1160 1428 Size: 422 738 268 Fragments arranged by size: 738 422 268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 305 336 1428 Size: 305 31 1092 Fragments arranged by size: 1092 305 31 MscI TGG'CCA Cuts at: 0 760 1428 Size: 760 668 MslI CAynn'nnrTG Cuts at: 0 344 1180 1428 Size: 344 836 248 Fragments arranged by size: 836 344 248 MspA1I CmG'CkG Cuts at: 0 100 1428 Size: 100 1328 NaeI GCC'GGC Cuts at: 0 121 1428 Size: 121 1307 NarI GG'CG_CC Cuts at: 0 71 94 117 1428 Size: 71 23 23 1311 Fragments arranged by size: 1311 71 23 23 NcoI C'CATG_G Cuts at: 0 741 1428 Size: 741 687 NdeI CA'TA_TG Cuts at: 0 771 1428 Size: 771 657 NgoMIV G'CCGG_C Cuts at: 0 119 1428 Size: 119 1309 NsiI A_TGCA'T Cuts at: 0 341 1428 Size: 341 1087 PasI CC'CwG_GG Cuts at: 0 546 1428 Size: 546 882 PflMI CCAn_nnn'nTGG Cuts at: 0 840 1428 Size: 840 588 PshAI GACnn'nnGTC Cuts at: 0 424 1428 Size: 424 1004 PstI C_TGCA'G Cuts at: 0 131 206 1428 Size: 131 75 1222 Fragments arranged by size: 1222 131 75 SacI G_AGCT'C Cuts at: 0 263 1428 Size: 263 1165 SacII CC_GC'GG Cuts at: 0 101 1428 Size: 101 1327 SfcI C'TryA_G Cuts at: 0 127 202 1428 Size: 127 75 1226 Fragments arranged by size: 1226 127 75 SfoI GGC'GCC Cuts at: 0 72 95 118 1428 Size: 72 23 23 1310 Fragments arranged by size: 1310 72 23 23 SgrAI Cr'CCGG_yG Cuts at: 0 119 1428 Size: 119 1309 SmaI CCC'GGG Cuts at: 0 90 847 1428 Size: 90 757 581 Fragments arranged by size: 757 581 90 SmlI C'TyrA_G Cuts at: 0 514 1105 1428 Size: 514 591 323 Fragments arranged by size: 591 514 323 SrfI GCCC'GGGC Cuts at: 0 847 1428 Size: 847 581 StyI C'CwwG_G Cuts at: 0 686 741 1428 Size: 686 55 687 Fragments arranged by size: 687 686 55 SwaI ATTT'AAAT Cuts at: 0 321 1428 Size: 321 1107 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 740 772 777 809 1428 Size: 740 32 5 32 619 Fragments arranged by size: 740 619 32 32 5 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1179 1428 Size: 1179 249 XmaI C'CCGG_G Cuts at: 0 88 845 1428 Size: 88 757 583 Fragments arranged by size: 757 583 88 Enzymes that do cut: AcuI AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI HaeII Hin4I HincII HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI PasI PflMI PshAI PstI SacI SacII SfcI SfoI SgrAI SmaI SmlI SrfI StyI SwaI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BglI BmgBI BmtI BsaBI BsgI BsiEI BsiWI BspEI BspMI BsrBI BsrGI BstBI BstZ17I Bsu36I BtgZI ClaI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI MluI NheI NotI NruI NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SspI StuI TaqII TatI Tth111I XbaI XhoI XmnI ZraI