Restriction map for
pFN21AE1083
(Circular) (Six-base) MAPSORT of: pFN21AE1083.seq Check: 5665 from: 1 to: 5815
pFN21AE1083 5815 bp
With 182 enzymes: SgfI *
September 25, 2009 17:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2820 3035 3878 5262 5530 1632
Size: 1188 215 843 1384 268 1917
Fragments arranged by size:
1917 1384 1188 843 268 215
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 4714 5691 1203
Size: 3511 977 1327
Fragments arranged by size:
3511 1327 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 3558 5130 3558
Size: 1572 4243
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 2404 3124 3350 3502 5611 1493
Size: 911 720 226 152 2109 1697
Fragments arranged by size:
2109 1697 911 720 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2429 4369 160
Size: 872 1397 1940 1606
Fragments arranged by size:
1940 1606 1397 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 3140 1870
Size: 73 1197 4545
Fragments arranged by size:
4545 1197 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3136 4591 618
Size: 325 217 627 1349 1455 1842
Fragments arranged by size:
1842 1455 1349 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2701 3134 1391
Size: 1310 433 4072
Fragments arranged by size:
4072 1310 433
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3048 928
Size: 971 1149 3695
Fragments arranged by size:
3695 1149 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4006 4040 1903
Size: 34 2069 34 3678
Fragments arranged by size:
3678 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BfrBI ATG'CAT
Cuts at: 2563 2563
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4426 136
Size: 107 122 71 631 605 2754 1525
Fragments arranged by size:
2754 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2746 5810 2746
Size: 3064 2751
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 3876 5032 1790
Size: 2086 1156 2573
Fragments arranged by size:
2573 2086 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3546 4504 442
Size: 416 53 83 399 24 2129 958 1753
Fragments arranged by size:
2129 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4475 1239
Size: 3236 2579
Bpu10I CC'TnA_GC
Cuts at: 2309 2659 2309
Size: 350 5465
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2784 3940 4805 5103 2784
Size: 1156 865 298 3496
Fragments arranged by size:
3496 1156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3606 493
Size: 711 2402 2702
Fragments arranged by size:
2702 2402 711
BsaBI GATnn'nnATC
Cuts at: 2442 5809 2442
Size: 3367 2448
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3622 4004 4639 275
Size: 53 83 186 936 2089 382 635 1451
Fragments arranged by size:
2089 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5590
Fragments arranged by size:
5590 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2147 2172 2696 1186
Size: 958 3 25 524 4305
Fragments arranged by size:
4305 958 524 25 3
BseYI C'CCAG_C
Cuts at: 5018 5514 5018
Size: 496 5319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5242
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2050 3007 3876 3961 5032 2050
Size: 957 869 85 1071 2833
Fragments arranged by size:
2833 1071 957 869 85
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
BsmBI CGTCTCn'nnnn_
Cuts at: 2848 2848
Size: 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2701 3007 3134 3876 3961 5032
Size: 399 260 651 306 127 742 85 1071
Cuts at: 5032 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 651 399 306 260 127
85
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 3704 5434 3704
Size: 1730 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3170 844
Size: 2326 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3702 1394
Size: 429 1879 3507
Fragments arranged by size:
3507 1879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2053 2254 4310 4484 65
Size: 1183 805 201 2056 174 1396
Fragments arranged by size:
2056 1396 1183 805 201 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4459 4646 1700
Size: 110 2649 187 2869
Fragments arranged by size:
2869 2649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 2021 3875 4887 2021
Size: 1854 1012 2949
Fragments arranged by size:
2949 1854 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5815
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5815
BstYI r'GATC_y
Cuts at: 1972 2746 3145 3512 3897 3914 5366 5452 5464
Size: 774 399 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 774 399 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 513
Size: 552 939 90 4234
Fragments arranged by size:
4234 939 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5076
Fragments arranged by size:
5076 683 56
BtsI GCAGTG_nn'
Cuts at: 3331 4125 4145 3331
Size: 794 20 5001
Fragments arranged by size:
5001 794 20
ClaI AT'CG_AT
Cuts at: 1554 2316 1554
Size: 762 5053
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 3119 3119
Size: 5815
DraIII CAC_nnn'GTG
Cuts at: 2045 2045
Size: 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3262 4151 5780
Size: 54 1425 50 275 18 1432 889 1629
Cuts at: 5780 8
Size: 43
Fragments arranged by size:
1629 1432 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2835 2846 3745 1353
Size: 1482 11 899 3423
Fragments arranged by size:
3423 1482 899 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2820 3035 3878 4475 5262 5530 1239
Size: 393 1188 215 843 597 787 268 1524
Fragments arranged by size:
1524 1188 843 787 597 393 268 215
EcoO109I rG'GnC_Cy
Cuts at: 2950 2950
Size: 5815
EcoRI G'AATT_C
Cuts at: 2404 3124 3502 2404
Size: 720 378 4717
Fragments arranged by size:
4717 720 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2057 3065 2057
Size: 1008 4807
FspI TGC'GCA
Cuts at: 4321 4321
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 4962 1678
Size: 3284 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2956 2988 4456 4488 4530
Size: 32 135 32 1384 32 1468 32 42
Cuts at: 4530 4562 1373
Size: 32 2626
Fragments arranged by size:
2626 1468 1384 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3405 669
Size: 988 281 1221 246 3079
Fragments arranged by size:
3079 1221 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 2109 3414 2109
Size: 1305 4510
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2578 4929 5113 1305
Size: 1273 2351 184 2007
Fragments arranged by size:
2351 2007 1273 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3993
Fragments arranged by size:
3993 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2341 2974 2976 3773 4132 4291 518
Size: 648 1175 633 2 797 359 159 2042
Fragments arranged by size:
2042 1175 797 648 633 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2122 3059 3231 3908 5056 5301 1606
Size: 516 937 172 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 677 516 245 172
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4324
Fragments arranged by size:
4324 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NsiI A_TGCA'T
Cuts at: 2565 2565
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 3049 3173 4718 1132
Size: 97 1820 124 1545 2229
Fragments arranged by size:
2229 1820 1545 124 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2950 2950
Size: 5815
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 2243 2352 3385 2243
Size: 109 1033 4673
Fragments arranged by size:
4673 1033 109
PstI C_TGCA'G
Cuts at: 830 2541 3167 830
Size: 1711 626 3478
Fragments arranged by size:
3478 1711 626
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
PvuII CAG'CTG
Cuts at: 2122 3059 2122
Size: 937 4878
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SapI GCTCTTCn'nnn_
Cuts at: 2835 2835
Size: 5815
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SfcI C'TryA_G
Cuts at: 826 1045 2537 3163 4298 4979 5170 826
Size: 219 1492 626 1135 681 191 1471
Fragments arranged by size:
1492 1471 1135 681 626 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2799 3919 4820 5082 820
Size: 197 926 856 1120 901 262 1553
Fragments arranged by size:
1553 1120 926 901 856 262 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 2165 152
Size: 2013 3802
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 2084 3739 5
Size: 47 2032 1655 2081
Fragments arranged by size:
2081 2032 1655 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5815
StyI C'CwwG_G
Cuts at: 513 1065 2004 3254 513
Size: 552 939 1250 3074
Fragments arranged by size:
3074 1250 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3842 3859 4012 4197 4642 325
Size: 1255 2262 17 153 185 445 1498
Fragments arranged by size:
2262 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4061 5773 96
Size: 274 80 33 51 1443 2084 1712 138
Fragments arranged by size:
2084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5775 5807 5775
Size: 32 5783
Tth111I GACn'n_nGTC
Cuts at: 1316 3067 1316
Size: 1751 4064
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 2844 3944 2844
Size: 1100 4715
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI