(Circular) (Six-base) MAPSORT of: pFN21AB8501.seq Check: 6591 from: 1 to: 7513 pFN21AB8501 7513 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5323 278 Size: 53 83 186 936 3787 2468 Fragments arranged by size: 3787 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2372 3049 4490 4856 1937 Size: 435 677 1441 366 4594 Fragments arranged by size: 4594 1441 677 435 366 Acc65I G'GTAC_C Cuts at: 4834 4834 Size: 7513 AclI AA'CG_TT Cuts at: 1563 5640 6013 1563 Size: 4077 373 3063 Fragments arranged by size: 4077 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2921 3651 4827 5576 6960 7228 1632 Size: 1289 730 1176 749 1384 268 1917 Fragments arranged by size: 1917 1384 1289 1176 749 730 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7316 AflIII A'CryG_T Cuts at: 1203 4450 6412 7389 1203 Size: 3247 1962 977 1327 Fragments arranged by size: 3247 1962 1327 977 AgeI A'CCGG_T Cuts at: 6344 6344 Size: 7513 AhdI GACnn_n'nnGTC Cuts at: 3853 6242 3853 Size: 2389 5124 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7513 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7513 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4104 4136 4104 Size: 32 7481 AlwNI CAG_nnn'CTG Cuts at: 2180 5256 6828 2180 Size: 3076 1572 2865 Fragments arranged by size: 3076 2865 1572 ApaLI G'TGCA_C Cuts at: 5570 6726 5570 Size: 1156 6357 ApoI r'AATT_y Cuts at: 1493 2299 2610 2662 2695 2786 2864 2875 3216 Size: 806 311 52 33 91 78 11 341 Cuts at: 3216 3262 3491 3671 3697 3920 4198 4617 4822 Size: 46 229 180 26 223 278 419 205 Cuts at: 4822 5048 5200 7309 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 806 419 341 311 278 229 226 223 205 180 152 91 78 52 46 33 26 11 AseI AT'TA_AT Cuts at: 160 1032 2976 3411 4274 6067 160 Size: 872 1944 435 863 1793 1606 Fragments arranged by size: 1944 1793 1606 872 863 435 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7513 AvaI C'yCGr_G Cuts at: 1870 1943 3606 4838 1870 Size: 73 1663 1232 4545 Fragments arranged by size: 4545 1663 1232 73 BamHI G'GATC_C Cuts at: 3298 4717 4843 5210 3298 Size: 1419 126 367 5601 Fragments arranged by size: 5601 1419 367 126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4834 6289 618 Size: 325 217 627 3047 1455 1842 Fragments arranged by size: 3047 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4832 1391 Size: 3441 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3372 3569 928 Size: 971 1473 197 4872 Fragments arranged by size: 4872 1473 971 197 BbvCI CC'TCA_GC Cuts at: 2009 2009 Size: 7513 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5704 5738 1903 Size: 34 50 34 3683 34 3678 Fragments arranged by size: 3683 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4425 5405 6615 4425 Size: 980 1210 5323 Fragments arranged by size: 5323 1210 980 BclI T'GATC_A Cuts at: 1548 2595 2685 3655 4032 1548 Size: 1047 90 970 377 5029 Fragments arranged by size: 5029 1047 970 377 90 BfrBI ATG'CAT Cuts at: 2778 4435 2778 Size: 1657 5856 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6124 136 Size: 107 122 71 631 605 4452 1525 Fragments arranged by size: 4452 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2019 7508 2019 Size: 5489 2024 BlpI GC'TnA_GC Cuts at: 4930 4930 Size: 7513 Bme1580I G_kGCm'C Cuts at: 1790 5574 6730 1790 Size: 3784 1156 2573 Fragments arranged by size: 3784 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2172 1318 Size: 854 6659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2557 5244 6202 Size: 416 53 83 399 24 1140 2687 958 Cuts at: 6202 442 Size: 1753 Fragments arranged by size: 2687 1753 1140 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7513 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 3060 6173 1239 Size: 854 967 3113 2579 Fragments arranged by size: 3113 2579 967 854 Bpu10I CC'TnA_GC Cuts at: 2009 2009 Size: 7513 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2229 4450 4736 5638 6503 6801 2229 Size: 2221 286 902 865 298 2941 Fragments arranged by size: 2941 2221 902 865 298 286 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2152 6176 882 Size: 617 653 4024 2219 Fragments arranged by size: 4024 2219 653 617 BsaAI yAC'GTr Cuts at: 493 1204 5304 493 Size: 711 4100 2702 Fragments arranged by size: 4100 2702 711 BsaBI GATnn'nnATC Cuts at: 7507 7507 Size: 7513 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5320 5702 6337 275 Size: 53 83 186 936 3787 382 635 1451 Fragments arranged by size: 3787 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2190 5946 6344 6618 6765 7176 2190 Size: 3756 398 274 147 411 2527 Fragments arranged by size: 3756 2527 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2798 2828 3019 3049 1178 Size: 30 165 30 1395 30 191 30 5642 Fragments arranged by size: 5642 1395 191 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7513 BseYI C'CCAG_C Cuts at: 6716 7212 6716 Size: 496 7017 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2313 2439 3877 1163 Size: 573 577 126 1438 4799 Fragments arranged by size: 4799 1438 577 573 126 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2191 4963 5724 6752 1540 Size: 275 186 190 2772 761 1028 2301 Fragments arranged by size: 2772 2301 1028 761 275 190 186 BsiHKAI G_wGCw'C Cuts at: 5574 5659 6730 5574 Size: 85 1071 6357 Fragments arranged by size: 6357 1071 85 BsmI GAATG_Cn' Cuts at: 5024 5117 5024 Size: 93 7420 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4832 5574 5659 6730 1391 Size: 399 3042 742 85 1071 2174 Fragments arranged by size: 3042 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7176 7176 Size: 7513 BspHI T'CATG_A Cuts at: 5402 7132 5402 Size: 1730 5783 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4868 844 Size: 4024 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2076 5400 1394 Size: 429 253 3324 3507 Fragments arranged by size: 3507 3324 429 253 BsrDI GCAATG_nn' Cuts at: 65 1248 3826 6008 6182 65 Size: 1183 2578 2182 174 1396 Fragments arranged by size: 2578 2182 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6157 6344 1700 Size: 110 4347 187 2869 Fragments arranged by size: 4347 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7513 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7513 BssSI C'ACGA_G Cuts at: 2331 5573 6585 2331 Size: 3242 1012 3259 Fragments arranged by size: 3259 3242 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2840 794 Size: 2046 5467 BstBI TT'CG_AA Cuts at: 6317 6317 Size: 7513 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7513 BstYI r'GATC_y Cuts at: 1972 2019 3298 4717 4843 5210 5595 5612 7064 Size: 47 1279 1419 126 367 385 17 1452 Cuts at: 7064 7150 7162 7508 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1419 1279 385 367 346 126 86 47 17 12 BstZ17I GTA'TAC Cuts at: 4491 4491 Size: 7513 Bsu36I CC'TnA_GG Cuts at: 2082 2082 Size: 7513 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6961 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6774 Fragments arranged by size: 6774 683 56 BtsI GCAGTG_nn' Cuts at: 3477 5029 5823 5843 3477 Size: 1552 794 20 5147 Fragments arranged by size: 5147 1552 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7513 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4669 4704 529 Size: 35 4105 35 3338 Fragments arranged by size: 4105 3338 35 35 DraI TTT'AAA Cuts at: 2538 3306 4074 4817 2538 Size: 768 768 743 5234 Fragments arranged by size: 5234 768 768 743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6520 809 Size: 507 5204 1802 Fragments arranged by size: 5204 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2237 4960 5849 Size: 54 1425 50 275 18 407 2723 889 Cuts at: 5849 7478 8 Size: 1629 43 Fragments arranged by size: 2723 1629 1425 889 407 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4960 1537 Size: 275 3148 4090 Fragments arranged by size: 4090 3148 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4515 5443 1353 Size: 3162 928 3423 Fragments arranged by size: 3423 3162 928 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6100 6474 6620 387 Size: 1064 138 4511 374 146 1280 Fragments arranged by size: 4511 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2921 3060 3651 4827 5576 6173 Size: 393 461 828 139 591 1176 749 597 Cuts at: 6173 6960 7228 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1176 828 787 749 597 591 461 393 268 139 EcoNI CCTnn'n_nnAGG Cuts at: 2804 2804 Size: 7513 EcoO109I rG'GnC_Cy Cuts at: 2087 2087 Size: 7513 EcoRI G'AATT_C Cuts at: 2662 2786 4822 5200 2662 Size: 124 2036 378 4975 Fragments arranged by size: 4975 2036 378 124 EcoRV GAT'ATC Cuts at: 3552 4189 3552 Size: 637 6876 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2340 3146 2340 Size: 806 6707 FspI TGC'GCA Cuts at: 6019 6019 Size: 7513 HaeII r_GCGC'y Cuts at: 1678 6660 1678 Size: 4982 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2798 2830 2933 Size: 32 135 32 437 32 757 32 103 Cuts at: 2933 2965 3603 3635 3839 3871 4541 4573 6154 Size: 32 638 32 204 32 670 32 1581 Cuts at: 6154 6186 6228 6260 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1581 757 670 638 437 204 135 103 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3100 4857 5103 669 Size: 988 281 1162 1757 246 3079 Fragments arranged by size: 3079 1757 1162 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7513 HpaI GTT'AAC Cuts at: 3100 5103 3100 Size: 2003 5510 KpnI G_GTAC'C Cuts at: 4838 4838 Size: 7513 MfeI C'AATT_G Cuts at: 4572 4712 5112 4572 Size: 140 400 6973 Fragments arranged by size: 6973 400 140 MluI A'CGCG_T Cuts at: 7389 7389 Size: 7513 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6627 6811 1305 Size: 5322 184 2007 Fragments arranged by size: 5322 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5691 Fragments arranged by size: 5691 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3623 4404 5471 5830 5989 518 Size: 648 2457 781 1067 359 159 2042 Fragments arranged by size: 2457 2042 1067 781 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2837 4929 5606 6754 6999 1606 Size: 1231 2092 677 1148 245 2120 Fragments arranged by size: 2120 2092 1231 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6961 NdeI CA'TA_TG Cuts at: 387 2584 4117 387 Size: 2197 1533 3783 Fragments arranged by size: 3783 2197 1533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7513 NotI GC'GGCC_GC Cuts at: 4960 4960 Size: 7513 NsiI A_TGCA'T Cuts at: 2780 4437 2780 Size: 1657 5856 NspI r_CATG'y Cuts at: 1132 1229 4435 4454 4871 6416 1132 Size: 97 3206 19 417 1545 2229 Fragments arranged by size: 3206 2229 1545 417 97 19 PacI TTA_AT'TAA Cuts at: 2976 3729 2976 Size: 753 6760 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7513 PciI A'CATG_T Cuts at: 4450 6412 4450 Size: 1962 5551 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7513 PfoI T'CCnGG_A Cuts at: 2109 3037 4386 2109 Size: 928 1349 5236 Fragments arranged by size: 5236 1349 928 PmeI GTTT'AAAC Cuts at: 4817 4817 Size: 7513 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5582 5614 7095 7127 5582 Size: 32 1481 32 5968 Fragments arranged by size: 5968 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2087 2087 Size: 7513 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7513 PsiI TTA'TAA Cuts at: 2400 5083 2400 Size: 2683 4830 PspXI vC'TCGA_Gb Cuts at: 3606 3606 Size: 7513 PstI C_TGCA'G Cuts at: 830 4549 4865 830 Size: 3719 316 3478 Fragments arranged by size: 3719 3478 316 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7513 PvuII CAG'CTG Cuts at: 2837 2837 Size: 7513 SalI G'TCGA_C Cuts at: 1936 4855 1936 Size: 2919 4594 SanDI GG'GwC_CC Cuts at: 2087 2087 Size: 7513 SbfI CC_TGCA'GG Cuts at: 4865 4865 Size: 7513 ScaI AGT'ACT Cuts at: 4177 5761 7473 4177 Size: 1584 1712 4217 Fragments arranged by size: 4217 1712 1584 SfcI C'TryA_G Cuts at: 826 1045 2989 3630 4545 4861 5996 6677 6868 Size: 219 1944 641 915 316 1135 681 191 Cuts at: 6868 826 Size: 1471 Fragments arranged by size: 1944 1471 1135 915 681 641 316 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7513 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7513 SmaI CCC'GGG Cuts at: 1872 4840 1872 Size: 2968 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2244 3606 4465 4751 5617 6518 Size: 197 926 301 1362 859 286 866 901 Cuts at: 6518 6780 820 Size: 262 1553 Fragments arranged by size: 1553 1362 926 901 866 859 301 286 262 197 SnaBI TAC'GTA Cuts at: 493 5304 493 Size: 4811 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7513 SphI G_CATG'C Cuts at: 1132 4871 1132 Size: 3739 3774 SspI AAT'ATT Cuts at: 5 52 4133 5437 5 Size: 47 4081 1304 2081 Fragments arranged by size: 4081 2081 1304 47 StuI AGG'CCT Cuts at: 1517 2014 1517 Size: 497 7016 StyI C'CwwG_G Cuts at: 513 1065 3285 3360 4587 4952 513 Size: 552 2220 75 1227 365 3074 Fragments arranged by size: 3074 2220 1227 552 365 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2125 5540 5557 5710 5895 6340 325 Size: 1255 545 3415 17 153 185 445 1498 Fragments arranged by size: 3415 1498 1255 545 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2760 4175 4761 Size: 274 80 33 51 1443 783 1415 586 Cuts at: 4761 5759 7471 96 Size: 998 1712 138 Fragments arranged by size: 1712 1443 1415 998 783 586 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7473 7505 7473 Size: 32 7481 Tth111I GACn'n_nGTC Cuts at: 1316 3233 1316 Size: 1917 5596 XbaI T'CTAG_A Cuts at: 4849 4849 Size: 7513 XhoI C'TCGA_G Cuts at: 1943 3606 1943 Size: 1663 5850 XmaI C'CCGG_G Cuts at: 1870 4838 1870 Size: 2968 4545 XmnI GAAnn'nnTTC Cuts at: 2735 2868 3129 3822 4773 4791 5642 2735 Size: 133 261 693 951 18 851 4606 Fragments arranged by size: 4606 951 851 693 261 133 18 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5321 276 Size: 53 83 186 936 3787 2468 Fragments arranged by size: 3787 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI