(Circular) (Six-base) MAPSORT of: pFN21AB9985.seq Check: 907 from: 1 to: 6421 pFN21AB9985 6421 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3308 3308 Size: 6421 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3198 4231 278 Size: 53 83 186 936 1662 1033 2468 Fragments arranged by size: 2468 1662 1033 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2640 3764 1937 Size: 703 1124 4594 Fragments arranged by size: 4594 1124 703 Acc65I G'GTAC_C Cuts at: 3742 3742 Size: 6421 AclI AA'CG_TT Cuts at: 1563 4548 4921 1563 Size: 2985 373 3063 Fragments arranged by size: 3063 2985 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2914 4484 5868 6136 1632 Size: 1282 1570 1384 268 1917 Fragments arranged by size: 1917 1570 1384 1282 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6224 AflIII A'CryG_T Cuts at: 1203 2907 5320 6297 1203 Size: 1704 2413 977 1327 Fragments arranged by size: 2413 1704 1327 977 AgeI A'CCGG_T Cuts at: 5252 5252 Size: 6421 AhdI GACnn_n'nnGTC Cuts at: 5150 5150 Size: 6421 AleI CACnn'nnGTG Cuts at: 1166 2912 1166 Size: 1746 4675 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6421 AlwNI CAG_nnn'CTG Cuts at: 3062 4164 5736 3062 Size: 1102 1572 3747 Fragments arranged by size: 3747 1572 1102 ApaLI G'TGCA_C Cuts at: 4478 5634 4478 Size: 1156 5265 ApoI r'AATT_y Cuts at: 1493 2518 2749 3704 3730 3956 4108 6217 1493 Size: 1025 231 955 26 226 152 2109 1697 Fragments arranged by size: 2109 1697 1025 955 231 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 4975 160 Size: 872 3943 1606 Fragments arranged by size: 3943 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 AvaI C'yCGr_G Cuts at: 1870 1943 3664 3746 1870 Size: 73 1721 82 4545 Fragments arranged by size: 4545 1721 82 73 BamHI G'GATC_C Cuts at: 3682 3751 4118 3682 Size: 69 367 5985 Fragments arranged by size: 5985 367 69 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2444 3208 3742 5197 618 Size: 325 217 627 657 764 534 1455 1842 Fragments arranged by size: 1842 1455 764 657 627 534 325 217 BanII G_rGCy'C Cuts at: 1391 2279 3740 1391 Size: 888 1461 4072 Fragments arranged by size: 4072 1461 888 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2139 2461 2524 2653 928 Size: 971 240 322 63 129 4696 Fragments arranged by size: 4696 971 322 240 129 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4612 4646 1903 Size: 34 2675 34 3678 Fragments arranged by size: 3678 2675 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4313 5523 4313 Size: 1210 5211 BclI T'GATC_A Cuts at: 1548 1548 Size: 6421 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5032 136 Size: 107 122 71 631 605 3360 1525 Fragments arranged by size: 3360 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2776 6416 2776 Size: 3640 2781 BlpI GC'TnA_GC Cuts at: 3838 3838 Size: 6421 Bme1580I G_kGCm'C Cuts at: 1790 4482 5638 1790 Size: 2692 1156 2573 Fragments arranged by size: 2692 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6421 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2177 4152 5110 Size: 416 53 83 399 24 760 1975 958 Cuts at: 5110 442 Size: 1753 Fragments arranged by size: 1975 1753 958 760 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3101 5081 1239 Size: 1862 1980 2579 Fragments arranged by size: 2579 1980 1862 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4546 5411 5709 4546 Size: 865 298 5258 Fragments arranged by size: 5258 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3462 5084 882 Size: 617 1963 1622 2219 Fragments arranged by size: 2219 1963 1622 617 BsaAI yAC'GTr Cuts at: 493 1204 2695 2910 4212 493 Size: 711 1491 215 1302 2702 Fragments arranged by size: 2702 1491 1302 711 215 BsaBI GATnn'nnATC Cuts at: 6415 6415 Size: 6421 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3195 4228 4610 5245 Size: 53 83 186 936 1662 1033 382 635 Cuts at: 5245 275 Size: 1451 Fragments arranged by size: 1662 1451 1033 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4854 5252 5526 5673 6084 4854 Size: 398 274 147 411 5191 Fragments arranged by size: 5191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2220 2250 3705 3735 1178 Size: 30 165 30 817 30 1455 30 3864 Fragments arranged by size: 3864 1455 817 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6421 BseYI C'CCAG_C Cuts at: 2089 3472 5624 6120 2089 Size: 1383 2152 496 2390 Fragments arranged by size: 2390 2152 1383 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 1163 Size: 573 522 5326 Fragments arranged by size: 5326 573 522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2746 3871 4632 5660 1540 Size: 275 186 745 1125 761 1028 2301 Fragments arranged by size: 2301 1125 1028 761 745 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 2919 3446 4482 4567 5638 2127 Size: 792 527 1036 85 1071 2910 Fragments arranged by size: 2910 1071 1036 792 527 85 BsmI GAATG_Cn' Cuts at: 3261 3932 4025 3261 Size: 671 93 5657 Fragments arranged by size: 5657 671 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3361 3361 Size: 6421 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2279 2919 3446 3740 4482 4567 Size: 399 337 152 640 527 294 742 85 Cuts at: 4567 5638 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 640 527 399 337 294 152 85 BspEI T'CCGG_A Cuts at: 6084 6084 Size: 6421 BspHI T'CATG_A Cuts at: 2080 2806 4310 6040 2080 Size: 726 1504 1730 2461 Fragments arranged by size: 2461 1730 1504 726 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3070 3257 3308 3776 844 Size: 2226 187 51 468 3489 Fragments arranged by size: 3489 2226 468 187 51 BsrBI CCG'CTC Cuts at: 1394 1823 4308 1394 Size: 429 2485 3507 Fragments arranged by size: 3507 2485 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2101 4916 5090 65 Size: 1183 853 2815 174 1396 Fragments arranged by size: 2815 1396 1183 853 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2447 3205 5065 5252 1700 Size: 110 637 758 1860 187 2869 Fragments arranged by size: 2869 1860 758 637 187 110 BsrGI T'GTAC_A Cuts at: 96 3558 96 Size: 3462 2959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6421 BssSI C'ACGA_G Cuts at: 2270 3439 4481 5493 2270 Size: 1169 1042 1012 3198 Fragments arranged by size: 3198 1169 1042 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6421 BstBI TT'CG_AA Cuts at: 5225 5225 Size: 6421 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6421 BstXI CCAn_nnnn'nTGG Cuts at: 2543 2543 Size: 6421 BstYI r'GATC_y Cuts at: 1972 2776 3682 3751 4118 4503 4520 5972 6058 Size: 804 906 69 367 385 17 1452 86 Cuts at: 6058 6070 6416 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 906 804 385 367 346 86 69 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3212 3267 513 Size: 552 939 1208 55 3667 Fragments arranged by size: 3667 1208 939 552 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2656 487 Size: 683 56 1430 4252 Fragments arranged by size: 4252 1430 683 56 BtsI GCAGTG_nn' Cuts at: 3937 4731 4751 3937 Size: 794 20 5607 Fragments arranged by size: 5607 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6421 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6386 DraI TTT'AAA Cuts at: 3709 3725 3709 Size: 16 6405 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2416 5428 809 Size: 507 1100 3012 1802 Fragments arranged by size: 3012 1802 1100 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2449 3127 3525 Size: 54 1425 50 275 18 619 678 398 Cuts at: 3525 3868 4757 6386 8 Size: 343 889 1629 43 Fragments arranged by size: 1629 1425 889 678 619 398 343 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3868 1537 Size: 275 2056 4090 Fragments arranged by size: 4090 2056 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4351 1353 Size: 2998 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2964 5008 5382 5528 387 Size: 1064 138 1375 2044 374 146 1280 Fragments arranged by size: 2044 1375 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2914 3101 4484 5081 5868 6136 1239 Size: 393 1282 187 1383 597 787 268 1524 Fragments arranged by size: 1524 1383 1282 787 597 393 268 187 EcoO109I rG'GnC_Cy Cuts at: 2966 3059 2966 Size: 93 6328 EcoRI G'AATT_C Cuts at: 3730 4108 3730 Size: 378 6043 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2872 2872 Size: 6421 FspI TGC'GCA Cuts at: 4927 4927 Size: 6421 HaeII r_GCGC'y Cuts at: 1678 3504 5568 1678 Size: 1826 2064 2531 Fragments arranged by size: 2531 2064 1826 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2794 2826 3461 Size: 32 135 32 450 32 740 32 635 Cuts at: 3461 3493 5062 5094 5136 5168 1373 Size: 32 1569 32 42 32 2626 Fragments arranged by size: 2626 1569 740 635 450 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3175 3765 4011 669 Size: 988 281 1237 590 246 3079 Fragments arranged by size: 3079 1237 988 590 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6421 HpaI GTT'AAC Cuts at: 3175 4011 3175 Size: 836 5585 KpnI G_GTAC'C Cuts at: 3746 3746 Size: 6421 MfeI C'AATT_G Cuts at: 3150 4020 3150 Size: 870 5551 MluI A'CGCG_T Cuts at: 6297 6297 Size: 6421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2781 5535 5719 1305 Size: 1476 2754 184 2007 Fragments arranged by size: 2754 2007 1476 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3129 3527 10 Size: 54 1425 343 1297 398 2904 Fragments arranged by size: 2904 1425 1297 398 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2630 2910 2912 3185 4379 4738 4897 Size: 648 1464 280 2 273 1194 359 159 Cuts at: 4897 518 Size: 2042 Fragments arranged by size: 2042 1464 1194 648 359 280 273 159 2 MspA1I CmG'CkG Cuts at: 1606 3837 4514 5662 5907 1606 Size: 2231 677 1148 245 2120 Fragments arranged by size: 2231 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3212 513 Size: 552 939 1208 3722 Fragments arranged by size: 3722 1208 939 552 NdeI CA'TA_TG Cuts at: 387 2662 387 Size: 2275 4146 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6421 NotI GC'GGCC_GC Cuts at: 3868 3868 Size: 6421 NspI r_CATG'y Cuts at: 1132 1229 3779 5324 1132 Size: 97 2550 1545 2229 Fragments arranged by size: 2550 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3412 1348 Size: 2064 4357 PciI A'CATG_T Cuts at: 5320 5320 Size: 6421 PflMI CCAn_nnn'nTGG Cuts at: 1108 2563 1108 Size: 1455 4966 PfoI T'CCnGG_A Cuts at: 2219 2219 Size: 6421 PmeI GTTT'AAAC Cuts at: 3725 3725 Size: 6421 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4490 4522 6003 6035 4490 Size: 32 1481 32 4876 Fragments arranged by size: 4876 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2966 3059 2966 Size: 93 6328 PshAI GACnn'nnGTC Cuts at: 1613 3194 1613 Size: 1581 4840 PsiI TTA'TAA Cuts at: 3991 3991 Size: 6421 PstI C_TGCA'G Cuts at: 830 2366 2959 3329 3773 830 Size: 1536 593 370 444 3478 Fragments arranged by size: 3478 1536 593 444 370 PvuI CG_AT'CG Cuts at: 2001 2746 2001 Size: 745 5676 SalI G'TCGA_C Cuts at: 1936 3763 1936 Size: 1827 4594 SbfI CC_TGCA'GG Cuts at: 3773 3773 Size: 6421 ScaI AGT'ACT Cuts at: 4669 6381 4669 Size: 1712 4709 SfcI C'TryA_G Cuts at: 826 1045 2362 2641 2955 3325 3769 4904 5585 Size: 219 1317 279 314 370 444 1135 681 Cuts at: 5585 5776 826 Size: 191 1471 Fragments arranged by size: 1471 1317 1135 681 444 370 314 279 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6421 SmaI CCC'GGG Cuts at: 1872 3666 3748 1872 Size: 1794 82 4545 Fragments arranged by size: 4545 1794 82 SmlI C'TyrA_G Cuts at: 820 1017 1943 4525 5426 5688 820 Size: 197 926 2582 901 262 1553 Fragments arranged by size: 2582 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4212 493 Size: 3719 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6421 SphI G_CATG'C Cuts at: 1132 3779 1132 Size: 2647 3774 SspI AAT'ATT Cuts at: 5 52 4345 5 Size: 47 4293 2081 Fragments arranged by size: 4293 2081 47 StuI AGG'CCT Cuts at: 1517 3624 1517 Size: 2107 4314 StyI C'CwwG_G Cuts at: 513 1065 2004 3212 3253 3860 513 Size: 552 939 1208 41 607 3074 Fragments arranged by size: 3074 1208 939 607 552 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3007 3326 4448 4465 4618 4803 5248 Size: 1255 1427 319 1122 17 153 185 445 Cuts at: 5248 325 Size: 1498 Fragments arranged by size: 1498 1427 1255 1122 445 319 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3558 4667 6379 Size: 274 80 33 51 1443 1581 1109 1712 Cuts at: 6379 96 Size: 138 Fragments arranged by size: 1712 1581 1443 1109 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2220 2252 3335 3367 3382 3414 6381 6413 2220 Size: 32 1083 32 15 32 2967 32 2228 Fragments arranged by size: 2967 2228 1083 32 32 32 32 15 Tth111I GACn'n_nGTC Cuts at: 1316 2970 3239 1316 Size: 1654 269 4498 Fragments arranged by size: 4498 1654 269 XbaI T'CTAG_A Cuts at: 3757 3757 Size: 6421 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6421 XmaI C'CCGG_G Cuts at: 1870 3664 3746 1870 Size: 1794 82 4545 Fragments arranged by size: 4545 1794 82 XmnI GAAnn'nnTTC Cuts at: 4550 4550 Size: 6421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3196 4229 276 Size: 53 83 186 936 1662 1033 2468 Fragments arranged by size: 2468 1662 1033 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI