Restriction map for
pFN21AB9985
(Linear) (Six-base) MAPSORT of: KIBB9985.seq Check: 1968 from: 1 to: 1716
KIBB9985.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1303 1716
Size: 1303 413
AatII G_ACGT'C
Cuts at: 0 1193 1716
Size: 1193 523
AccI GT'mk_AC
Cuts at: 0 635 1716
Size: 635 1081
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 909 1716
Size: 909 807
AflIII A'CryG_T
Cuts at: 0 902 1716
Size: 902 814
AleI CACnn'nnGTG
Cuts at: 0 907 1716
Size: 907 809
AlwNI CAG_nnn'CTG
Cuts at: 0 1057 1716
Size: 1057 659
ApoI r'AATT_y
Cuts at: 0 513 744 1699 1716
Size: 513 231 955 17
Fragments arranged by size:
955 513 231 17
AvaI C'yCGr_G
Cuts at: 0 1659 1716
Size: 1659 57
BamHI G'GATC_C
Cuts at: 0 1677 1716
Size: 1677 39
BanI G'GyrC_C
Cuts at: 0 439 1203 1716
Size: 439 764 513
Fragments arranged by size:
764 513 439
BanII G_rGCy'C
Cuts at: 0 274 1716
Size: 274 1442
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 456 519 648 1716
Size: 134 322 63 129 1068
Fragments arranged by size:
1068 322 134 129 63
BglII A'GATC_T
Cuts at: 0 771 1716
Size: 771 945
BmrI ACTGGGnnnn_n'
Cuts at: 0 172 1716
Size: 172 1544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1716
Size: 1096 620
BsaI GGTCTCn'nnnn_
Cuts at: 0 1457 1716
Size: 1457 259
BsaAI yAC'GTr
Cuts at: 0 690 905 1716
Size: 690 215 811
Fragments arranged by size:
811 690 215
BsaHI Gr'CG_yC
Cuts at: 0 1190 1716
Size: 1190 526
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 215 245 1716
Size: 215 30 1471
Fragments arranged by size:
1471 215 30
BseYI C'CCAG_C
Cuts at: 0 84 1467 1716
Size: 84 1383 249
Fragments arranged by size:
1383 249 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1716
Size: 253 1463
BsiEI CG_ry'CG
Cuts at: 0 741 1716
Size: 741 975
BsiHKAI G_wGCw'C
Cuts at: 0 122 914 1441 1716
Size: 122 792 527 275
Fragments arranged by size:
792 527 275 122
BsmI GAATG_Cn'
Cuts at: 0 1256 1716
Size: 1256 460
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1356 1716
Size: 1356 360
Bsp1286I G_dGCh'C
Cuts at: 0 122 274 914 1441 1716
Size: 122 152 640 527 275
Fragments arranged by size:
640 527 275 152 122
BspHI T'CATG_A
Cuts at: 0 75 801 1716
Size: 75 726 915
Fragments arranged by size:
915 726 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1065 1252 1303 1716
Size: 1065 187 51 413
Fragments arranged by size:
1065 413 187 51
BsrDI GCAATG_nn'
Cuts at: 0 96 1716
Size: 96 1620
BsrFI r'CCGG_y
Cuts at: 0 442 1200 1716
Size: 442 758 516
Fragments arranged by size:
758 516 442
BsrGI T'GTAC_A
Cuts at: 0 1553 1716
Size: 1553 163
BssSI C'ACGA_G
Cuts at: 0 265 1434 1716
Size: 265 1169 282
Fragments arranged by size:
1169 282 265
BstXI CCAn_nnnn'nTGG
Cuts at: 0 538 1716
Size: 538 1178
BstYI r'GATC_y
Cuts at: 0 771 1677 1716
Size: 771 906 39
Fragments arranged by size:
906 771 39
BtgI C'CryG_G
Cuts at: 0 1207 1262 1716
Size: 1207 55 454
Fragments arranged by size:
1207 454 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 651 1716
Size: 651 1065
DraI TTT'AAA
Cuts at: 0 1704 1716
Size: 1704 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 411 1716
Size: 411 1305
EaeI y'GGCC_r
Cuts at: 0 444 1122 1520 1716
Size: 444 678 398 196
Fragments arranged by size:
678 444 398 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 959 1716
Size: 959 757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 909 1096 1716
Size: 909 187 620
Fragments arranged by size:
909 620 187
EcoO109I rG'GnC_Cy
Cuts at: 0 961 1054 1716
Size: 961 93 662
Fragments arranged by size:
961 662 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 867 1716
Size: 867 849
HaeII r_GCGC'y
Cuts at: 0 1499 1716
Size: 1499 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 789 821 1456 1488 1716
Size: 17 32 740 32 635 32 228
Fragments arranged by size:
740 635 228 32 32 32 17
HincII GTy'rAC
Cuts at: 0 1170 1716
Size: 1170 546
HpaI GTT'AAC
Cuts at: 0 1170 1716
Size: 1170 546
MfeI C'AATT_G
Cuts at: 0 1145 1716
Size: 1145 571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1716
Size: 776 940
MscI TGG'CCA
Cuts at: 0 1124 1522 1716
Size: 1124 398 194
Fragments arranged by size:
1124 398 194
MslI CAynn'nnrTG
Cuts at: 0 625 905 907 1180 1716
Size: 625 280 2 273 536
Fragments arranged by size:
625 536 280 273 2
NcoI C'CATG_G
Cuts at: 0 1207 1716
Size: 1207 509
NdeI CA'TA_TG
Cuts at: 0 657 1716
Size: 657 1059
PasI CC'CwG_GG
Cuts at: 0 1407 1716
Size: 1407 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 558 1716
Size: 558 1158
PfoI T'CCnGG_A
Cuts at: 0 214 1716
Size: 214 1502
PpuMI rG'GwC_Cy
Cuts at: 0 961 1054 1716
Size: 961 93 662
Fragments arranged by size:
961 662 93
PshAI GACnn'nnGTC
Cuts at: 0 1189 1716
Size: 1189 527
PstI C_TGCA'G
Cuts at: 0 361 954 1324 1716
Size: 361 593 370 392
Fragments arranged by size:
593 392 370 361
PvuI CG_AT'CG
Cuts at: 0 741 1716
Size: 741 975
SfcI C'TryA_G
Cuts at: 0 357 636 950 1320 1716
Size: 357 279 314 370 396
Fragments arranged by size:
396 370 357 314 279
SmaI CCC'GGG
Cuts at: 0 1661 1716
Size: 1661 55
StuI AGG'CCT
Cuts at: 0 1619 1716
Size: 1619 97
StyI C'CwwG_G
Cuts at: 0 1207 1248 1716
Size: 1207 41 468
Fragments arranged by size:
1207 468 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1002 1321 1716
Size: 1002 319 395
Fragments arranged by size:
1002 395 319
TatI w'GTAC_w
Cuts at: 0 1553 1716
Size: 1553 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 215 247 1330 1362 1377 1409 1716
Size: 215 32 1083 32 15 32 307
Fragments arranged by size:
1083 307 215 32 32 32 15
Tth111I GACn'n_nGTC
Cuts at: 0 965 1234 1716
Size: 965 269 482
Fragments arranged by size:
965 482 269
XmaI C'CCGG_G
Cuts at: 0 1659 1716
Size: 1659 57
ZraI GAC'GTC
Cuts at: 0 1191 1716
Size: 1191 525
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlwNI ApoI
AvaI BamHI BanI BanII BbsI BglII BmrI BpmI
BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstXI BstYI BtgI BtgZI DraI DrdI EaeI
EciI Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI
MfeI MmeI MscI MslI NcoI NdeI PasI PflMI
PfoI PpuMI PshAI PstI PvuI SfcI SmaI StuI
StyI TaqII TatI TstI Tth111I XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsaWI BseRI
BsiWI BspEI BsrBI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtsI ClaI CspCI DraIII EagI EarI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII KasI
KpnI MluI MspA1I NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XcmI XhoI XmnI