################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MslI MunI NarI NciI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Alw44I 91 GTGCAC G^TGCAC AlwI 186 GATCC GGATC(4/5) ApaLI 91 GTGCAC G^TGCAC AscI 139 GGCGCGCC GG^CGCGCC BalI 257 TGGCCA TGG^CCA BbsI 357 GAAGAC GAAGAC(2/6) BfaI 59 CTAG C^TAG BlpI 121 GCTGAGC GC^T[ACGT]AGC Bpu1102I 121 GCTGAGC GC^T[ACGT]AGC BpuAI 357 GAAGAC GAAGAC(2/6) BsaJI 38 CCAAGG C^C[ACGT][ACGT]GG BssHII 140 GCGCGC G^CGCGC BstAPI 93 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 185 AGATCC [GA]^GATC[CT] CelII 121 GCTGAGC GC^T[ACGT]AGC Cfr13I 134 GGGCC G^G[ACGT]CC DpnII 186 GATC ^GATC EaeI 257 TGGCCA [CT]^GGCC[GA] EcoT14I 38 CCAAGG C^C[AT][AT]GG MaeI 59 CTAG C^TAG MaeII 9 ACGT A^CGT MaeIII 301 GTTAC ^GT[ACGT]AC MboI 186 GATC ^GATC MboII 357 GAAGA GAAGA(8/7) MflI 185 AGATCC [GA]^GATC[CT] MluNI 257 TGGCCA TGG^CCA MscI 257 TGGCCA TGG^CCA MspA1I 253 CAGCTG C[AC]G^C[GT]G NaeI 136 GCCGGC GCC^GGC NdeII 186 GATC ^GATC NgoMIV 136 GCCGGC G^CCGGC PvuII 253 CAGCTG CAG^CTG SacI 277 GAGCTC GAGCT^C Sau3AI 186 GATC ^GATC Sau96I 134 GGGCC G^G[ACGT]CC SfaNI 233 GCATC GCATC(5/9) SfcI 80 CTGTAG C^T[GA][CT]AG StyI 38 CCAAGG C^C[AT][AT]GG Tsp509I 74 AATT ^AATT XbaI 58 TCTAGA T^CTAGA XhoII 185 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 171 GTAC GT^AC 343 GTAC AspHI 91 GTGCAC G[AT]GC[AT]^C 277 GAGCTC BanII 67 GGGCTC G[GA]GC[CT]^C 277 GAGCTC BpmI 166 CTGGAG CTGGAG(16/14) 190 CTGGAG BseRI 54 CTCCTC GAGGAG(10/8) 274 GAGGAG BsiHKAI 91 GTGCAC G[AT]GC[AT]^C 277 GAGCTC BsiYI 153 CCTGGCGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 189 CCTGGAGCTGG BslI 153 CCTGGCGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 189 CCTGGAGCTGG BsrBI 51 CCGCTC CCGCTC(-3/-3) 124 GAGCGG BsrFI 136 GCCGGC [GA]^CCGG[CT] 144 GCCGGT Cfr10I 136 GCCGGC [GA]^CCGG[CT] 144 GCCGGT HapII 137 CCGG C^CGG 145 CCGG HpaII 137 CCGG C^CGG 145 CCGG MnlI 56 CCTC CCTC(7/6) 274 GAGG MspI 137 CCGG C^CGG 145 CCGG NlaIV 68 GGCTCC GG[ACGT]^[ACGT]CC 133 GGGGCC RsaI 171 GTAC GT^AC 343 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AccII AciI AluI BbvI BmyI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI DdeI EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I ItaI MvaI MvnI MwoI ScrFI TseI