Restriction map for
pFN21AB5887
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AvaII AviII AvrII BamHI BanII
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoT22I FauI
FbaI FseI FspI HgaI KpnI
KspI MaeII MamI MfeI MluI
MroI MslI MunI MvnI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 312 GTCTAC GT^[AC][GT]AC
AciI 183 GCGG CCGC(-3/-1)
AcyI 1325 GGCGCC G[GA]^CG[CT]C
AhdI 1397 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1183 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1397 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 462 CCCGAG C^[CT]CG[GA]G
BalI 517 TGGCCA TGG^CCA
BanI 1325 GGCGCC G^G[CT][GA]CC
BbeI 1325 GGCGCC GGCGC^C
BglII 1236 AGATCT A^GATCT
BsaHI 1325 GGCGCC G[GA]^CG[CT]C
BsgI 592 GTGCAG GTGCAG(16/14)
BsmI 1245 GAATGC GAATGC(1/-1)
BsoBI 462 CCCGAG C^[CT]CG[GA]G
BssSI 143 CACGAG CACGAG(-5/-1)
BstBI 710 TTCGAA TT^CGAA
BstEII 583 GGTGACC G^GT[ACGT]ACC
BstF5I 779 CATCC GGATG(2/0)
BstPI 583 GGTGACC G^GT[ACGT]ACC
CfoI 1326 GCGC GCG^C
Cfr13I 693 GGGCC G^G[ACGT]CC
DsaI 782 CCATGG C^C[GA][CT]GG
EaeI 517 TGGCCA [CT]^GGCC[GA]
Eam1105I 1397 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 583 GGTGACC G^GT[ACGT]ACC
EcoRV 1194 GATATC GAT^ATC
FokI 779 CATCC GGATG(9/13)
HaeII 1325 GGCGCC [GA]GCGC^[CT]
HapII 696 CCGG C^CGG
HhaI 1326 GCGC GCG^C
Hin1I 1325 GGCGCC G[GA]^CG[CT]C
HinP1I 1326 GCGC G^CGC
HindIII 1342 AAGCTT A^AGCTT
HpaI 835 GTTAAC GTT^AAC
HpaII 696 CCGG C^CGG
KasI 1325 GGCGCC G^GCGCC
MluNI 517 TGGCCA TGG^CCA
MscI 517 TGGCCA TGG^CCA
MspA1I 203 CAGCTG C[AC]G^C[GT]G
MspI 696 CCGG C^CGG
MwoI 1317 GCAGCTAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 1325 GGCGCC GG^CGCC
NcoI 782 CCATGG C^CATGG
NspV 710 TTCGAA TT^CGAA
PshAI 1435 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 41 CTGCAG CTGCA^G
PvuII 203 CAGCTG CAG^CTG
SapI 1312 GAAGAGC GCTCTTC(1/4)
Sau96I 693 GGGCC G^G[ACGT]CC
ScaI 1306 AGTACT AGT^ACT
SfoI 1325 GGCGCC GGC^GCC
SfuI 710 TTCGAA TT^CGAA
TaqI 711 TCGA T^CGA
TthHB8I 711 TCGA T^CGA
XmnI 1183 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
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AlwI 22 GGATC GGATC(4/5)
803 GGATC
BbsI 242 GAAGAC GAAGAC(2/6)
940 GAAGAC
BpuAI 242 GAAGAC GAAGAC(2/6)
940 GAAGAC
BsiYI 383 CCTTAGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1329 CCTGCAAGAGG
BslI 383 CCTTAGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1329 CCTGCAAGAGG
BsmAI 227 GAGAC GTCTC(1/5)
400 GAGAC
BspMI 39 ACCTGC ACCTGC(4/8)
432 GCAGGT
BssKI 647 CCTGG ^CC[ACGT]GG
973 CCAGG
BstNI 647 CCTGG CC^[AT]GG
973 CCAGG
Cac8I 849 GCTTGC GC[ACGT]^[ACGT]GC
1328 GCCTGC
EarI 73 GAAGAG CTCTTC(1/4)
1312 GAAGAG
EcoNI 383 CCTTAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1329 CCTGCAAGAGG
EcoRII 647 CCTGG ^CC[AT]GG
973 CCAGG
EcoT14I 782 CCATGG C^C[AT][AT]GG
946 CCTTGG
HincII 835 GTTAAC GT[CT]^[GA]AC
1432 GTTGAC
HindII 835 GTTAAC GT[CT]^[GA]AC
1432 GTTGAC
Ksp632I 73 GAAGAG CTCTTC(1/4)
1312 GAAGAG
MaeIII 584 GTGAC ^GT[ACGT]AC
1405 GTCAC
MvaI 647 CCTGG CC^[AT]GG
973 CCAGG
NspI 405 ACATGC [GA]CATG^[CT]
957 ACATGC
ScrFI 647 CCTGG CC^[ACGT]GG
973 CCAGG
SfaNI 724 GATGC GCATC(5/9)
130 GCATC
SfcI 41 CTGCAG C^T[GA][CT]AG
206 CTGTAG
SmlI 1042 CTTGAG C^T[CT][GA]AG
1219 CTTGAG
StyI 782 CCATGG C^C[AT][AT]GG
946 CCTTGG
TfiI 699 GAATC G^A[AT]TC
718 GAATC
Tsp45I 584 GTGAC ^GT[GC]AC
1405 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BciVI BfaI BsaJI BseRI BsmFI
BsrI BstYI DdeI DpnII Fnu4HI
HaeIII HinfI HphI ItaI MaeI
MboI MboII MflI MnlI MseI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI SspI Tru9I TseI Tsp509I
TspRI XhoII