################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI BpmI Bpu1102I BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AciI 444 CCGC CCGC(-3/-1) AcsI 764 GAATTT [GA]^AATT[CT] AflII 748 CTTAAG C^TTAAG AflIII 104 ACGTGT A^C[GA][CT]GT AlwNI 222 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 764 GAATTT [GA]^AATT[CT] BbsI 408 GTCTTC GAAGAC(2/6) BbvI 367 GCAGC GCAGC(8/12) BfrI 748 CTTAAG C^TTAAG BpuAI 408 GTCTTC GAAGAC(2/6) BsaAI 103 TACGTG [CT]AC^GT[GA] BsaBI 54 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiYI 28 CCCAGCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 28 CCCAGCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 81 GTCTC GTCTC(1/5) BsmI 480 GCATTC GAATGC(1/-1) BspMI 43 ACCTGC ACCTGC(4/8) BsrDI 159 CATTGC GCAATG(2/0) BstF5I 525 CATCC GGATG(2/0) EarI 208 GAAGAG CTCTTC(1/4) FokI 525 CATCC GGATG(9/13) HphI 514 GGTGA GGTGA(8/7) Ksp632I 208 GAAGAG CTCTTC(1/4) MaeII 104 ACGT A^CGT MamI 54 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PleI 713 GACTC GAGTC(4/5) PstI 76 CTGCAG CTGCA^G ScaI 143 AGTACT AGT^ACT TaqI 570 TCGA T^CGA TfiI 463 GAATC G^A[AT]TC TseI 367 GCAGC G^C[AT]GC Tsp509I 765 AATT ^AATT TspRI 68 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 570 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 108 GTAC GT^AC 144 GTAC AspHI 70 GTGCTC G[AT]GC[AT]^C 787 GAGCAC BfaI 337 CTAG C^TAG 677 CTAG BmyI 70 GTGCTC G[AGT]GC[ACT]^C 787 GAGCAC BsiHKAI 70 GTGCTC G[AT]GC[AT]^C 787 GAGCAC Bsp1286I 70 GTGCTC G[AGT]GC[ACT]^C 787 GAGCAC Cac8I 47 GCGTGC GC[ACGT]^[ACGT]GC 230 GCTGGC DpnII 54 GATC ^GATC 60 GATC Fnu4HI 367 GCAGC GC^[ACGT]GC 443 GCCGC HaeIII 442 GGCC GG^CC 828 GGCC HinfI 463 GAATC G^A[ACGT]TC 713 GACTC ItaI 367 GCAGC GC^[ACGT]GC 443 GCCGC MaeI 337 CTAG C^TAG 677 CTAG MaeIII 97 GTAAC ^GT[ACGT]AC 778 GTAAC MboI 54 GATC ^GATC 60 GATC MseI 172 TTAA T^TAA 749 TTAA NdeII 54 GATC ^GATC 60 GATC RsaI 108 GTAC GT^AC 144 GTAC Sau3AI 54 GATC ^GATC 60 GATC SfaNI 132 GATGC GCATC(5/9) 700 GATGC SfcI 76 CTGCAG C^T[GA][CT]AG 302 CTACAG SmlI 504 CTTGAG C^T[CT][GA]AG 748 CTTAAG SspI 325 AATATT AAT^ATT 738 AATATT Tru9I 172 TTAA T^TAA 749 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AluI AvaII BsaJI BsmFI BsrI BssKI BstNI Cfr13I DdeI DraII EcoO109I EcoRII MboII MnlI MvaI NlaIII NlaIV PpuMI Sau96I ScrFI