(Circular) (Six-base) MAPSORT of: pFN21AE1038.seq Check: 3402 from: 1 to: 6721 pFN21AE1038 6721 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2029 2029 Size: 6721 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4531 278 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4064 1937 Size: 2127 4594 Acc65I G'GTAC_C Cuts at: 4042 4042 Size: 6721 AclI AA'CG_TT Cuts at: 1563 4848 5221 1563 Size: 3285 373 3063 Fragments arranged by size: 3285 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3579 3612 4784 6168 6436 1632 Size: 1947 33 1172 1384 268 1917 Fragments arranged by size: 1947 1917 1384 1172 268 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6524 AflIII A'CryG_T Cuts at: 1203 5620 6597 1203 Size: 4417 977 1327 Fragments arranged by size: 4417 1327 977 AgeI A'CCGG_T Cuts at: 5552 5552 Size: 6721 AhdI GACnn_n'nnGTC Cuts at: 5450 5450 Size: 6721 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6721 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6721 AlwNI CAG_nnn'CTG Cuts at: 2267 4464 6036 2267 Size: 2197 1572 2952 Fragments arranged by size: 2952 2197 1572 ApaLI G'TGCA_C Cuts at: 4778 5934 4778 Size: 1156 5565 ApoI r'AATT_y Cuts at: 1493 2247 2697 3027 3155 3271 3429 4030 4256 Size: 754 450 330 128 116 158 601 226 Cuts at: 4256 4408 6517 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 754 601 450 330 226 158 152 128 116 AseI AT'TA_AT Cuts at: 160 1032 3006 5275 160 Size: 872 1974 2269 1606 Fragments arranged by size: 2269 1974 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 AvaI C'yCGr_G Cuts at: 1870 1943 2189 4046 1870 Size: 73 246 1857 4545 Fragments arranged by size: 4545 1857 246 73 BamHI G'GATC_C Cuts at: 4051 4418 4051 Size: 367 6354 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3342 4042 5497 618 Size: 325 217 627 1555 700 1455 1842 Fragments arranged by size: 1842 1555 1455 700 627 325 217 BanII G_rGCy'C Cuts at: 1391 2967 4040 1391 Size: 1576 1073 4072 Fragments arranged by size: 4072 1576 1073 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2066 2625 3093 928 Size: 971 167 559 468 4556 Fragments arranged by size: 4556 971 559 468 167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4912 4946 1903 Size: 34 2975 34 3678 Fragments arranged by size: 3678 2975 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4613 5823 4613 Size: 1210 5511 BclI T'GATC_A Cuts at: 1548 2848 3505 1548 Size: 1300 657 4764 Fragments arranged by size: 4764 1300 657 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3399 5332 136 Size: 107 122 71 631 605 1727 1933 1525 Fragments arranged by size: 1933 1727 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6716 6716 Size: 6721 BlpI GC'TnA_GC Cuts at: 4138 4138 Size: 6721 Bme1580I G_kGCm'C Cuts at: 1790 4782 5938 1790 Size: 2992 1156 2573 Fragments arranged by size: 2992 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6721 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2225 4452 5410 Size: 416 53 83 399 24 808 2227 958 Cuts at: 5410 442 Size: 1753 Fragments arranged by size: 2227 1753 958 808 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2634 3594 3863 5381 1239 Size: 1395 960 269 1518 2579 Fragments arranged by size: 2579 1518 1395 960 269 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2068 3356 4846 5711 6009 2068 Size: 1288 1490 865 298 2780 Fragments arranged by size: 2780 1490 1288 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5384 882 Size: 617 3885 2219 Fragments arranged by size: 3885 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4512 493 Size: 711 3308 2702 Fragments arranged by size: 3308 2702 711 BsaBI GATnn'nnATC Cuts at: 6715 6715 Size: 6721 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4528 4910 5545 275 Size: 53 83 186 936 2995 382 635 1451 Fragments arranged by size: 2995 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5154 5552 5826 5973 6384 5154 Size: 398 274 147 411 5491 Fragments arranged by size: 5491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3609 3639 3678 3708 3836 Size: 30 165 30 2206 30 39 30 128 Cuts at: 3836 3866 1178 Size: 30 4033 Fragments arranged by size: 4033 2206 165 128 39 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2733 1186 Size: 1547 5174 BseYI C'CCAG_C Cuts at: 2940 5924 6420 2940 Size: 2984 496 3241 Fragments arranged by size: 3241 2984 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3893 3981 1163 Size: 573 2157 88 3903 Fragments arranged by size: 3903 2157 573 88 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4171 4932 5960 1540 Size: 275 186 2170 761 1028 2301 Fragments arranged by size: 2301 2170 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2520 4782 4867 5938 2520 Size: 2262 85 1071 3303 Fragments arranged by size: 3303 2262 1071 85 BsmI GAATG_Cn' Cuts at: 4232 4325 4232 Size: 93 6628 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2520 2967 4040 4782 4867 5938 1391 Size: 399 730 447 1073 742 85 1071 2174 Fragments arranged by size: 2174 1073 1071 742 730 447 399 85 BspEI T'CCGG_A Cuts at: 6384 6384 Size: 6721 BspHI T'CATG_A Cuts at: 2122 3508 3907 4610 6340 2122 Size: 1386 399 703 1730 2503 Fragments arranged by size: 2503 1730 1386 703 399 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2029 4003 4076 844 Size: 1185 1974 73 3489 Fragments arranged by size: 3489 1974 1185 73 BsrBI CCG'CTC Cuts at: 1394 1823 4608 1394 Size: 429 2785 3507 Fragments arranged by size: 3507 2785 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5216 5390 65 Size: 1183 3968 174 1396 Fragments arranged by size: 3968 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5365 5552 1700 Size: 110 3555 187 2869 Fragments arranged by size: 3555 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6721 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6721 BssSI C'ACGA_G Cuts at: 4781 5793 4781 Size: 1012 5709 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6721 BstBI TT'CG_AA Cuts at: 5525 5525 Size: 6721 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6721 BstYI r'GATC_y Cuts at: 1972 2008 2534 4051 4418 4803 4820 6272 6358 Size: 36 526 1517 367 385 17 1452 86 Cuts at: 6358 6370 6716 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1517 1452 526 385 367 346 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2238 2238 Size: 6721 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5230 Fragments arranged by size: 5230 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5982 Fragments arranged by size: 5982 683 56 BtsI GCAGTG_nn' Cuts at: 2178 4237 5031 5051 2178 Size: 2059 794 20 3848 Fragments arranged by size: 3848 2059 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6721 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6686 DraI TTT'AAA Cuts at: 2019 3700 3822 3891 4025 2019 Size: 1681 122 69 134 4715 Fragments arranged by size: 4715 1681 134 122 69 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5728 809 Size: 507 4412 1802 Fragments arranged by size: 4412 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4168 5057 6686 Size: 54 1425 50 275 18 2338 889 1629 Cuts at: 6686 8 Size: 43 Fragments arranged by size: 2338 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4168 1537 Size: 275 2356 4090 Fragments arranged by size: 4090 2356 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3951 4651 1353 Size: 2598 700 3423 Fragments arranged by size: 3423 2598 700 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3443 5308 5682 5828 387 Size: 1064 138 1854 1865 374 146 1280 Fragments arranged by size: 1865 1854 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2634 3579 3594 3612 3863 4784 5381 Size: 393 1002 945 15 18 251 921 597 Cuts at: 5381 6168 6436 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1002 945 921 787 597 393 268 251 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 3612 3612 Size: 6721 EcoRI G'AATT_C Cuts at: 3429 4030 4408 3429 Size: 601 378 5742 Fragments arranged by size: 5742 601 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2792 3758 2792 Size: 966 5755 FspI TGC'GCA Cuts at: 5227 5227 Size: 6721 HaeII r_GCGC'y Cuts at: 1678 5868 1678 Size: 4190 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2211 2243 3293 3325 5362 Size: 32 135 32 639 32 1050 32 2037 Cuts at: 5362 5394 5436 5468 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2037 1050 639 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4065 4311 669 Size: 988 281 2127 246 3079 Fragments arranged by size: 3079 2127 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6721 HpaI GTT'AAC Cuts at: 4311 4311 Size: 6721 KpnI G_GTAC'C Cuts at: 4046 4046 Size: 6721 MfeI C'AATT_G Cuts at: 3384 4320 3384 Size: 936 5785 MluI A'CGCG_T Cuts at: 6597 6597 Size: 6721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3030 5835 6019 1305 Size: 1725 2805 184 2007 Fragments arranged by size: 2805 2007 1725 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4899 Fragments arranged by size: 4899 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2400 4679 5038 5197 518 Size: 648 1234 2279 359 159 2042 Fragments arranged by size: 2279 2042 1234 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3297 4137 4814 5962 6207 1606 Size: 1691 840 677 1148 245 2120 Fragments arranged by size: 2120 1691 1148 840 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5230 Fragments arranged by size: 5230 939 552 NdeI CA'TA_TG Cuts at: 387 3415 387 Size: 3028 3693 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6721 NotI GC'GGCC_GC Cuts at: 4168 4168 Size: 6721 NspI r_CATG'y Cuts at: 1132 1229 4079 5624 1132 Size: 97 2850 1545 2229 Fragments arranged by size: 2850 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6721 PciI A'CATG_T Cuts at: 5620 5620 Size: 6721 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6721 PfoI T'CCnGG_A Cuts at: 2563 2563 Size: 6721 PmeI GTTT'AAAC Cuts at: 4025 4025 Size: 6721 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4790 4822 6303 6335 4790 Size: 32 1481 32 5176 Fragments arranged by size: 5176 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6721 PsiI TTA'TAA Cuts at: 4291 4291 Size: 6721 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2823 2855 2823 Size: 32 6689 PstI C_TGCA'G Cuts at: 830 2040 2263 4073 830 Size: 1210 223 1810 3478 Fragments arranged by size: 3478 1810 1210 223 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6721 PvuII CAG'CTG Cuts at: 3297 3297 Size: 6721 SalI G'TCGA_C Cuts at: 1936 4063 1936 Size: 2127 4594 SbfI CC_TGCA'GG Cuts at: 4073 4073 Size: 6721 ScaI AGT'ACT Cuts at: 4969 6681 4969 Size: 1712 5009 SfcI C'TryA_G Cuts at: 826 1045 2036 2259 3676 3971 4069 5204 5885 Size: 219 991 223 1417 295 98 1135 681 Cuts at: 5885 6076 826 Size: 191 1471 Fragments arranged by size: 1471 1417 1135 991 681 295 223 219 191 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6721 SmaI CCC'GGG Cuts at: 1872 2191 4048 1872 Size: 319 1857 4545 Fragments arranged by size: 4545 1857 319 SmlI C'TyrA_G Cuts at: 820 1017 1943 2083 3371 4825 5726 5988 820 Size: 197 926 140 1288 1454 901 262 1553 Fragments arranged by size: 1553 1454 1288 926 901 262 197 140 SnaBI TAC'GTA Cuts at: 493 4512 493 Size: 4019 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6721 SphI G_CATG'C Cuts at: 1132 4079 1132 Size: 2947 3774 SspI AAT'ATT Cuts at: 5 52 4645 5 Size: 47 4593 2081 Fragments arranged by size: 4593 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6721 StyI C'CwwG_G Cuts at: 513 1065 2004 4160 513 Size: 552 939 2156 3074 Fragments arranged by size: 3074 2156 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2744 3562 3849 4748 4765 4918 5103 Size: 1255 1164 818 287 899 17 153 185 Cuts at: 5103 5548 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1164 899 818 445 287 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4967 6679 96 Size: 274 80 33 51 1443 2990 1712 138 Fragments arranged by size: 2990 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2526 2558 3609 3641 6681 6713 2526 Size: 32 1051 32 3040 32 2534 Fragments arranged by size: 3040 2534 1051 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6721 XbaI T'CTAG_A Cuts at: 3652 3952 4057 3652 Size: 300 105 6316 Fragments arranged by size: 6316 300 105 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2688 2688 Size: 6721 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6721 XmaI C'CCGG_G Cuts at: 1870 2189 4046 1870 Size: 319 1857 4545 Fragments arranged by size: 4545 1857 319 XmnI GAAnn'nnTTC Cuts at: 2835 4850 2835 Size: 2015 4706 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4529 276 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI