Restriction map for
pFN21AE1038
(Linear) (Six-base) MAPSORT of: KIEE1038.seq Check: 1283 from: 1 to: 2016
KIEE1038.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 24 2016
Size: 24 1992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1574 1607 2016
Size: 1574 33 409
Fragments arranged by size:
1574 409 33
AlwNI CAG_nnn'CTG
Cuts at: 0 262 2016
Size: 262 1754
ApoI r'AATT_y
Cuts at: 0 242 692 1022 1150 1266 1424 2016
Size: 242 450 330 128 116 158 592
Fragments arranged by size:
592 450 330 242 158 128 116
AseI AT'TA_AT
Cuts at: 0 1001 2016
Size: 1001 1015
AvaI C'yCGr_G
Cuts at: 0 184 2016
Size: 184 1832
BanI G'GyrC_C
Cuts at: 0 1337 2016
Size: 1337 679
BanII G_rGCy'C
Cuts at: 0 962 2016
Size: 962 1054
BbsI GAAGACnn'nnnn_
Cuts at: 0 61 620 1088 2016
Size: 61 559 468 928
Fragments arranged by size:
928 559 468 61
BclI T'GATC_A
Cuts at: 0 843 1500 2016
Size: 843 657 516
Fragments arranged by size:
843 657 516
BglI GCCn_nnn'nGGC
Cuts at: 0 1394 2016
Size: 1394 622
BmrI ACTGGGnnnn_n'
Cuts at: 0 220 2016
Size: 220 1796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 1589 1858 2016
Size: 629 960 269 158
Fragments arranged by size:
960 629 269 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1351 2016
Size: 63 1288 665
Fragments arranged by size:
1288 665 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1604 1634 1673 1703 1831 1861 2016
Size: 1604 30 39 30 128 30 155
Fragments arranged by size:
1604 155 128 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 728 2016
Size: 728 1288
BseYI C'CCAG_C
Cuts at: 0 935 2016
Size: 935 1081
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1888 1976 2016
Size: 1888 88 40
Fragments arranged by size:
1888 88 40
BsiHKAI G_wGCw'C
Cuts at: 0 515 2016
Size: 515 1501
Bsp1286I G_dGCh'C
Cuts at: 0 515 962 2016
Size: 515 447 1054
Fragments arranged by size:
1054 515 447
BspHI T'CATG_A
Cuts at: 0 117 1503 1902 2016
Size: 117 1386 399 114
Fragments arranged by size:
1386 399 117 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 1998 2016
Size: 24 1974 18
Fragments arranged by size:
1974 24 18
BstYI r'GATC_y
Cuts at: 0 3 529 2016
Size: 3 526 1487
Fragments arranged by size:
1487 526 3
Bsu36I CC'TnA_GG
Cuts at: 0 233 2016
Size: 233 1783
BtsI GCAGTG_nn'
Cuts at: 0 173 2016
Size: 173 1843
DraI TTT'AAA
Cuts at: 0 14 1695 1817 1886 2016
Size: 14 1681 122 69 130
Fragments arranged by size:
1681 130 122 69 14
EarI CTCTTCn'nnn_
Cuts at: 0 1946 2016
Size: 1946 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1438 2016
Size: 1438 578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 1574 1589 1607 1858 2016
Size: 629 945 15 18 251 158
Fragments arranged by size:
945 629 251 158 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1607 2016
Size: 1607 409
EcoRI G'AATT_C
Cuts at: 0 1424 2016
Size: 1424 592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 787 1753 2016
Size: 787 966 263
Fragments arranged by size:
966 787 263
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 206 238 1288 1320 2016
Size: 206 32 1050 32 696
Fragments arranged by size:
1050 696 206 32 32
MfeI C'AATT_G
Cuts at: 0 1379 2016
Size: 1379 637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 2016
Size: 1025 991
MslI CAynn'nnrTG
Cuts at: 0 395 2016
Size: 395 1621
MspA1I CmG'CkG
Cuts at: 0 1292 2016
Size: 1292 724
NdeI CA'TA_TG
Cuts at: 0 1410 2016
Size: 1410 606
PfoI T'CCnGG_A
Cuts at: 0 558 2016
Size: 558 1458
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 818 850 2016
Size: 818 32 1166
Fragments arranged by size:
1166 818 32
PstI C_TGCA'G
Cuts at: 0 35 258 2016
Size: 35 223 1758
Fragments arranged by size:
1758 223 35
PvuII CAG'CTG
Cuts at: 0 1292 2016
Size: 1292 724
SfcI C'TryA_G
Cuts at: 0 31 254 1671 1966 2016
Size: 31 223 1417 295 50
Fragments arranged by size:
1417 295 223 50 31
SmaI CCC'GGG
Cuts at: 0 186 2016
Size: 186 1830
SmlI C'TyrA_G
Cuts at: 0 78 1366 2016
Size: 78 1288 650
Fragments arranged by size:
1288 650 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 739 1557 1844 2016
Size: 739 818 287 172
Fragments arranged by size:
818 739 287 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 521 553 1604 1636 2016
Size: 521 32 1051 32 380
Fragments arranged by size:
1051 521 380 32 32
XbaI T'CTAG_A
Cuts at: 0 1647 1947 2016
Size: 1647 300 69
Fragments arranged by size:
1647 300 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 683 2016
Size: 683 1333
XmaI C'CCGG_G
Cuts at: 0 184 2016
Size: 184 1832
XmnI GAAnn'nnTTC
Cuts at: 0 830 2016
Size: 830 1186
Enzymes that do cut:
AarI AcuI AlwNI ApoI AseI AvaI BanI BanII
BbsI BclI BglI BmrI BpmI BpuEI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BstYI Bsu36I
BtsI DraI EarI EciI Eco57MI EcoNI EcoRI FalI
Hin4I MfeI MmeI MslI MspA1I NdeI PfoI PsrI
PstI PvuII SfcI SmaI SmlI TaqII TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EcoICRI EcoO109I EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI Tth111I XhoI
ZraI