################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT22I FokI FseI FspI HaeII HpaI KasI KpnI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 1428 GTCTAC GT^[AC][GT]AC AcyI 1282 GGCGTC G[GA]^CG[CT]C AfaI 141 GTAC GT^AC BamHI 7 GGATCC G^GATCC BciVI 1167 GGATAC GTATCC(6/5) BclI 644 TGATCA T^GATCA BglI 263 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 1282 GGCGTC G[GA]^CG[CT]C BspMI 1325 ACCTGC ACCTGC(4/8) BsrFI 1046 ACCGGC [GA]^CCGG[CT] BstAPI 165 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 7 GGATCC [GA]^GATC[CT] Cac8I 951 GCCTGC GC[ACGT]^[ACGT]GC CfoI 53 GCGC GCG^C Cfr10I 1046 ACCGGC [GA]^CCGG[CT] DraI 255 TTTAAA TTT^AAA DraIII 166 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 110 CCGCGG C^C[GA][CT]GG EaeI 40 CGGCCG [CT]^GGCC[GA] EagI 40 CGGCCG C^GGCCG EclXI 40 CGGCCG C^GGCCG Eco52I 40 CGGCCG C^GGCCG EcoNI 715 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 916 GAATTC G^AATTC EcoT14I 942 CCAAGG C^C[AT][AT]GG FbaI 644 TGATCA T^GATCA HgaI 1283 GCGTC GACGC(5/10) HhaI 53 GCGC GCG^C Hin1I 1282 GGCGTC G[GA]^CG[CT]C HinP1I 53 GCGC G^CGC HincII 798 GTTGAC GT[CT]^[GA]AC HindII 798 GTTGAC GT[CT]^[GA]AC HindIII 554 AAGCTT A^AGCTT HphI 321 GGTGA GGTGA(8/7) KspI 110 CCGCGG CCGC^GG MflI 7 GGATCC [GA]^GATC[CT] MslI 532 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG RsaI 141 GTAC GT^AC SacII 110 CCGCGG CCGC^GG SexAI 167 ACCTGGT A^CC[AT]GGT SfcI 491 CTATAG C^T[GA][CT]AG SmlI 1227 CTTGAG C^T[CT][GA]AG StyI 942 CCAAGG C^C[AT][AT]GG XhoII 7 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### ApaI 200 GGGCCC GGGCC^C 402 GGGCCC AspHI 880 GAGCAC G[AT]GC[AT]^C 1321 GAGCAC AvaII 185 GGACC G^G[AT]CC 1434 GGTCC BanII 200 GGGCCC G[GA]GC[CT]^C 402 GGGCCC BcnI 27 CCGGG CC^[GC]GG 84 CCGGG BfaI 367 CTAG C^TAG 548 CTAG BpmI 749 CTGGAG CTGGAG(16/14) 1277 CTGGAG BseRI 101 GAGGAG GAGGAG(10/8) 1118 GAGGAG BsiHKAI 880 GAGCAC G[AT]GC[AT]^C 1321 GAGCAC DraII 401 AGGGCCC [GA]G^G[ACGT]CC[CT] 402 GGGCCCT DrdI 106 GACCCCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 766 GACCATGATGTC EarI 1089 CTCTTC CTCTTC(1/4) 341 GAAGAG EcoO109I 401 AGGGCCC [GA]G^G[ACGT]CC[CT] 402 GGGCCCT Ksp632I 1089 CTCTTC CTCTTC(1/4) 341 GAAGAG MaeI 367 CTAG C^TAG 548 CTAG MseI 256 TTAA T^TAA 1096 TTAA NciI 27 CCGGG CC^[GC]GG 84 CCGGG SspI 444 AATATT AAT^ATT 963 AATATT Tru9I 256 TTAA T^TAA 1096 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI BbvI BmyI BsaI BsaJI BsgI BsiEI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstNI BstUI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I