Restriction map for pFN21AE4488
 (Linear) (Six-base) MAPSORT of: KIEE4488.seq  Check: 9725  from: 1  to: 1530



KIEE4488.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1334    1530 
   Size:       1334     196
 
AccI GT'mk_AC

Cuts at:      0    1429    1530 
   Size:       1429     101
 
ApaI G_GGCC'C

Cuts at:      0     204     406    1530 
   Size:        204     202    1124

  Fragments arranged by size: 

                1124     204     202
 
ApoI r'AATT_y

Cuts at:      0     236     682     839     874     916    1221    1522    1530 
   Size:        236     446     157      35      42     305     301       8

  Fragments arranged by size: 

                 446     305     301     236     157      42      35       8
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1160    1193    1530 
   Size:       1160      33     337

  Fragments arranged by size: 

                1160     337      33
 
BamHI G'GATC_C

Cuts at:      0       7    1530 
   Size:          7    1523
 
BanII G_rGCy'C

Cuts at:      0     204     406    1530 
   Size:        204     202    1124

  Fragments arranged by size: 

                1124     204     202
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1161    1530 
   Size:       1161     369
 
BclI T'GATC_A

Cuts at:      0     644    1530 
   Size:        644     886
 
BglI GCCn_nnn'nGGC

Cuts at:      0     269    1530 
   Size:        269    1261
 
Bme1580I G_kGCm'C

Cuts at:      0     194     204     406    1530 
   Size:        194      10     202    1124

  Fragments arranged by size: 

                1124     202     194      10
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     318    1530 
   Size:        318    1212
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1125    1530 
   Size:       1125     405
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     770    1298    1530 
   Size:        770     528     232

  Fragments arranged by size: 

                 770     528     232
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1248    1530 
   Size:       1248     282
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      28      98    1502    1530 
   Size:         28      70    1404      28

  Fragments arranged by size: 

                1404      70      28      28
 
BsaHI Gr'CG_yC

Cuts at:      0    1283    1530 
   Size:       1283     247
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     116    1133    1530 
   Size:        116    1017     397

  Fragments arranged by size: 

                1017     397     116
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     701    1312    1381    1530 
   Size:        193     508     611      69     149

  Fragments arranged by size: 

                 611     508     193     149      69
 
BsiEI CG_ry'CG

Cuts at:      0      43      75     116    1530 
   Size:         43      32      41    1414

  Fragments arranged by size: 

                1414      43      41      32
 
BsiHKAI G_wGCw'C

Cuts at:      0     884    1325    1530 
   Size:        884     441     205

  Fragments arranged by size: 

                 884     441     205
 
BsmI GAATG_Cn'

Cuts at:      0     219    1133    1262    1530 
   Size:        219     914     129     268

  Fragments arranged by size: 

                 914     268     219     129
 
Bsp1286I G_dGCh'C

Cuts at:      0     194     204     406     884    1325    1530 
   Size:        194      10     202     478     441     205

  Fragments arranged by size: 

                 478     441     205     202     194      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1334    1530 
   Size:       1334     196
 
BsrFI r'CCGG_y

Cuts at:      0    1046    1530 
   Size:       1046     484
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     171    1530 
   Size:        171    1359
 
BstYI r'GATC_y

Cuts at:      0       7    1530 
   Size:          7    1523
 
BtgI C'CryG_G

Cuts at:      0     110    1530 
   Size:        110    1420
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     112    1530 
   Size:        112    1418
 
DraI TTT'AAA

Cuts at:      0     257    1530 
   Size:        257    1273
 
DraIII CAC_nnn'GTG

Cuts at:      0     171    1530 
   Size:        171    1359
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     112     772    1530 
   Size:        112     660     758

  Fragments arranged by size: 

                 758     660     112
 
EaeI y'GGCC_r

Cuts at:      0      40    1530 
   Size:         40    1490
 
EagI C'GGCC_G

Cuts at:      0      40    1530 
   Size:         40    1490
 
EarI CTCTTCn'nnn_

Cuts at:      0     336    1095    1530 
   Size:        336     759     435

  Fragments arranged by size: 

                 759     435     336
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     770    1298    1530 
   Size:        770     528     232

  Fragments arranged by size: 

                 770     528     232
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     719    1530 
   Size:        719     811
 
EcoO109I rG'GnC_Cy

Cuts at:      0     402     403    1530 
   Size:        402       1    1127

  Fragments arranged by size: 

                1127     402       1
 
EcoRI G'AATT_C

Cuts at:      0     916    1530 
   Size:        916     614
 
HincII GTy'rAC

Cuts at:      0     800    1530 
   Size:        800     730
 
HindIII A'AGCT_T

Cuts at:      0     554    1530 
   Size:        554     976
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     420    1530 
   Size:        420    1110
 
MslI CAynn'nnrTG

Cuts at:      0     536    1530 
   Size:        536     994
 
MspA1I CmG'CkG

Cuts at:      0      45     112     421    1041    1513    1530 
   Size:         45      67     309     620     472      17

  Fragments arranged by size: 

                 620     472     309      67      45      17
 
PspOMI G'GGCC_C

Cuts at:      0     200     402    1530 
   Size:        200     202    1128

  Fragments arranged by size: 

                1128     202     200
 
PvuII CAG'CTG

Cuts at:      0     421    1041    1513    1530 
   Size:        421     620     472      17

  Fragments arranged by size: 

                 620     472     421      17
 
SacII CC_GC'GG

Cuts at:      0     113    1530 
   Size:        113    1417
 
SexAI A'CCwGG_T

Cuts at:      0     167    1530 
   Size:        167    1363
 
SfcI C'TryA_G

Cuts at:      0     491    1530 
   Size:        491    1039
 
SmlI C'TyrA_G

Cuts at:      0    1227    1530 
   Size:       1227     303
 
SspI AAT'ATT

Cuts at:      0     446     965    1530 
   Size:        446     519     565

  Fragments arranged by size: 

                 565     519     446
 
StyI C'CwwG_G

Cuts at:      0     942    1530 
   Size:        942     588
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      61    1530 
   Size:         61    1469
 
TatI w'GTAC_w

Cuts at:      0     140    1530 
   Size:        140    1390
 

 Enzymes that do cut:

     AarI     AccI     ApaI     ApoI     BaeI    BamHI    BanII    BciVI
     BclI     BglI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaHI    BseRI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrFI   BstAPI    BstYI     BtgI    BtgZI     DraI   DraIII     DrdI
     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI   HincII
  HindIII     MmeI     MslI   MspA1I   PspOMI    PvuII    SacII    SexAI
     SfcI     SmlI     SspI     StyI    TaqII     TatI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BanI     BbeI     BbsI    BbvCI     BcgI
    BfrBI    BglII     BlpI    BmgBI     BmtI   Bpu10I    BsaAI    BsaBI
    BsaWI    BsaXI    BseYI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     EciI  EcoICRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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