(Circular) (Six-base) MAPSORT of: pFN21AE4503.seq Check: 5655 from: 1 to: 6289 pFN21AE4503 6289 bp With 182 enzymes: SgfI * March 28, 2012 14:05 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4099 278 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2377 2868 3632 1937 Size: 440 491 764 4594 Fragments arranged by size: 4594 764 491 440 Acc65I G'GTAC_C Cuts at: 3610 3610 Size: 6289 AclI AA'CG_TT Cuts at: 1563 4416 4789 1563 Size: 2853 373 3063 Fragments arranged by size: 3063 2853 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2182 3277 3320 3570 4352 5736 6004 1632 Size: 550 1095 43 250 782 1384 268 1917 Fragments arranged by size: 1917 1384 1095 782 550 268 250 43 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6092 AflIII A'CryG_T Cuts at: 1203 2141 5188 6165 1203 Size: 938 3047 977 1327 Fragments arranged by size: 3047 1327 977 938 AgeI A'CCGG_T Cuts at: 5120 5120 Size: 6289 AhdI GACnn_n'nnGTC Cuts at: 5018 5018 Size: 6289 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6289 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6289 AlwNI CAG_nnn'CTG Cuts at: 4032 5604 4032 Size: 1572 4717 ApaI G_GGCC'C Cuts at: 2694 3030 3413 3534 2694 Size: 336 383 121 5449 Fragments arranged by size: 5449 383 336 121 ApaLI G'TGCA_C Cuts at: 4346 5502 4346 Size: 1156 5133 ApoI r'AATT_y Cuts at: 1493 3598 3824 3976 6085 1493 Size: 2105 226 152 2109 1697 Fragments arranged by size: 2109 2105 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4843 160 Size: 872 3811 1606 Fragments arranged by size: 3811 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2425 2446 3189 3614 1870 Size: 73 73 409 21 743 425 4545 Fragments arranged by size: 4545 743 425 409 73 73 21 BamHI G'GATC_C Cuts at: 2285 3619 3986 2285 Size: 1334 367 4588 Fragments arranged by size: 4588 1334 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2111 2789 3281 3446 Size: 325 217 627 233 91 678 492 165 Cuts at: 3446 3610 5065 618 Size: 164 1455 1842 Fragments arranged by size: 1842 1455 678 627 492 325 233 217 165 164 91 BanII G_rGCy'C Cuts at: 1391 2012 2694 3030 3161 3266 3413 3534 3608 Size: 621 682 336 131 105 147 121 74 Cuts at: 3608 1391 Size: 4072 Fragments arranged by size: 4072 682 621 336 147 131 121 105 74 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5318 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3075 3109 4480 4514 1903 Size: 34 1138 34 1371 34 3678 Fragments arranged by size: 3678 1371 1138 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2623 4181 5391 2623 Size: 1558 1210 3521 Fragments arranged by size: 3521 1558 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6289 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2838 3417 3529 Size: 107 122 71 631 605 1166 579 112 Cuts at: 3529 4900 136 Size: 1371 1525 Fragments arranged by size: 1525 1371 1166 631 605 579 122 112 107 71 BglII A'GATC_T Cuts at: 6284 6284 Size: 6289 BlpI GC'TnA_GC Cuts at: 3706 3706 Size: 6289 Bme1580I G_kGCm'C Cuts at: 1790 2023 2049 2694 2794 3030 3413 3534 4350 Size: 233 26 645 100 236 383 121 816 Cuts at: 4350 5506 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 816 645 383 236 233 121 100 26 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6289 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2184 3414 4020 Size: 416 53 83 399 24 767 1230 606 Cuts at: 4020 4978 442 Size: 958 1753 Fragments arranged by size: 1753 1230 958 767 606 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3542 1056 Size: 2486 3803 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3593 Size: 6289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2173 2200 2251 2263 2817 2985 3040 3166 Size: 934 27 51 12 554 168 55 126 Cuts at: 3166 3289 3448 4949 1239 Size: 123 159 1501 2579 Fragments arranged by size: 2579 1501 934 554 168 159 126 123 55 51 27 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4414 5279 5577 4414 Size: 865 298 5126 Fragments arranged by size: 5126 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2081 4952 882 Size: 617 582 2871 2219 Fragments arranged by size: 2871 2219 617 582 BsaAI yAC'GTr Cuts at: 493 1204 4080 493 Size: 711 2876 2702 Fragments arranged by size: 2876 2702 711 BsaBI GATnn'nnATC Cuts at: 6283 6283 Size: 6289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4096 4478 5113 275 Size: 53 83 186 936 2563 382 635 1451 Fragments arranged by size: 2563 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2360 2390 3224 4722 5120 5394 5541 5952 2360 Size: 30 834 1498 398 274 147 411 2697 Fragments arranged by size: 2697 1498 834 411 398 274 147 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2198 2228 1178 Size: 30 165 30 795 30 5239 Fragments arranged by size: 5239 795 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2347 2896 3379 1186 Size: 883 278 549 483 4096 Fragments arranged by size: 4096 883 549 483 278 BseYI C'CCAG_C Cuts at: 2400 2404 2571 2723 3023 3074 3197 3534 5492 Size: 4 167 152 300 51 123 337 1958 Cuts at: 5492 5988 2400 Size: 496 2701 Fragments arranged by size: 2701 1958 496 337 300 167 152 123 51 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5716 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3739 4500 5528 1540 Size: 275 186 1738 761 1028 2301 Fragments arranged by size: 2301 1738 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4350 4435 5506 4350 Size: 85 1071 5133 Fragments arranged by size: 5133 1071 85 BsmI GAATG_Cn' Cuts at: 3104 3800 3893 3104 Size: 696 93 5500 Fragments arranged by size: 5500 696 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2019 2704 2019 Size: 685 5604 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2023 2049 2694 2794 3030 3161 Size: 399 222 11 26 645 100 236 131 Cuts at: 3161 3266 3413 3534 3608 4350 4435 5506 1391 Size: 105 147 121 74 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 645 399 236 222 147 131 121 105 100 85 74 26 11 BspEI T'CCGG_A Cuts at: 2360 5952 2360 Size: 3592 2697 BspHI T'CATG_A Cuts at: 4178 5908 4178 Size: 1730 4559 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3644 844 Size: 2800 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3492 4176 1394 Size: 429 1669 684 3507 Fragments arranged by size: 3507 1669 684 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2292 2830 3099 4784 4958 65 Size: 1183 1044 538 269 1685 174 1396 Fragments arranged by size: 1685 1396 1183 1044 538 269 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3284 4933 5120 1700 Size: 110 1474 1649 187 2869 Fragments arranged by size: 2869 1649 1474 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6289 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6289 BssSI C'ACGA_G Cuts at: 4349 5361 4349 Size: 1012 5277 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6289 BstBI TT'CG_AA Cuts at: 5093 5093 Size: 6289 BstEII G'GTnAC_C Cuts at: 1184 3368 1184 Size: 2184 4105 BstXI CCAn_nnnn'nTGG Cuts at: 2606 3140 3320 2606 Size: 534 180 5575 Fragments arranged by size: 5575 534 180 BstYI r'GATC_y Cuts at: 1972 2285 2315 2363 3480 3619 3986 4371 4388 Size: 313 30 48 1117 139 367 385 17 Cuts at: 4388 5840 5926 5938 6284 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1117 385 367 346 313 139 86 48 30 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2551 3153 513 Size: 552 939 547 602 3649 Fragments arranged by size: 3649 939 602 552 547 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5550 Fragments arranged by size: 5550 683 56 BtsI GCAGTG_nn' Cuts at: 2060 2626 2689 3256 3475 3805 4599 4619 2060 Size: 566 63 567 219 330 794 20 3730 Fragments arranged by size: 3730 794 567 566 330 219 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3439 3474 529 Size: 35 2875 35 3344 Fragments arranged by size: 3344 2875 35 35 DraI TTT'AAA Cuts at: 3593 3593 Size: 6289 DraIII CAC_nnn'GTG Cuts at: 2737 2737 Size: 6289 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5296 809 Size: 507 3980 1802 Fragments arranged by size: 3980 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2623 3096 3736 Size: 54 1425 50 275 18 793 473 640 Cuts at: 3736 4625 6254 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 793 640 473 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3736 1537 Size: 275 1924 4090 Fragments arranged by size: 4090 1924 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2593 2599 3367 4219 1353 Size: 1240 6 768 852 3423 Fragments arranged by size: 3423 1240 852 768 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4876 5250 5396 387 Size: 1064 138 3287 374 146 1280 Fragments arranged by size: 3287 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2182 2200 2251 2263 2817 2985 Size: 393 541 9 18 51 12 554 168 Cuts at: 2985 3040 3166 3277 3289 3320 3448 3570 4352 Size: 55 126 111 12 31 128 122 782 Cuts at: 4352 4949 5736 6004 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 782 597 554 541 393 268 168 128 126 122 111 55 51 31 18 12 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 2991 2991 Size: 6289 EcoO109I rG'GnC_Cy Cuts at: 2091 2134 2690 2810 3007 3027 3167 3409 3410 Size: 43 556 120 197 20 140 242 1 Cuts at: 3410 3530 3531 2091 Size: 120 1 4849 Fragments arranged by size: 4849 556 242 197 140 120 120 43 20 1 1 EcoRI G'AATT_C Cuts at: 3598 3976 3598 Size: 378 5911 EcoRV GAT'ATC Cuts at: 3505 3505 Size: 6289 FspI TGC'GCA Cuts at: 4795 4795 Size: 6289 HaeII r_GCGC'y Cuts at: 1678 5436 1678 Size: 3758 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2679 2711 4930 4962 5004 Size: 32 135 32 1107 32 2219 32 42 Cuts at: 5004 5036 1373 Size: 32 2626 Fragments arranged by size: 2626 2219 1107 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3633 3879 669 Size: 988 281 1695 246 3079 Fragments arranged by size: 3079 1695 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6289 HpaI GTT'AAC Cuts at: 3879 3879 Size: 6289 KpnI G_GTAC'C Cuts at: 3614 3614 Size: 6289 MfeI C'AATT_G Cuts at: 3888 3888 Size: 6289 MluI A'CGCG_T Cuts at: 2141 6165 2141 Size: 4024 2265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2664 5403 5587 1305 Size: 1359 2739 184 2007 Fragments arranged by size: 2739 2007 1359 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2625 10 Size: 54 1425 343 793 3674 Fragments arranged by size: 3674 1425 793 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4247 4606 4765 518 Size: 648 3081 359 159 2042 Fragments arranged by size: 3081 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2193 2283 2404 2436 2553 3023 3443 3705 Size: 587 90 121 32 117 470 420 262 Cuts at: 3705 4382 5530 5775 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 587 470 420 262 245 121 117 90 32 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4798 Fragments arranged by size: 4798 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6289 NheI G'CTAG_C Cuts at: 1052 3538 1052 Size: 2486 3803 NotI GC'GGCC_GC Cuts at: 3736 3736 Size: 6289 NspI r_CATG'y Cuts at: 1132 1229 3382 3647 5192 1132 Size: 97 2153 265 1545 2229 Fragments arranged by size: 2229 2153 1545 265 97 PasI CC'CwG_GG Cuts at: 1348 2299 1348 Size: 951 5338 PciI A'CATG_T Cuts at: 5188 5188 Size: 6289 PflMI CCAn_nnn'nTGG Cuts at: 1108 3115 1108 Size: 2007 4282 PfoI T'CCnGG_A Cuts at: 3498 3498 Size: 6289 PmeI GTTT'AAAC Cuts at: 3593 3593 Size: 6289 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4358 4390 5871 5903 4358 Size: 32 1481 32 4744 Fragments arranged by size: 4744 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2091 2134 2810 3007 3167 2091 Size: 43 676 197 160 5213 Fragments arranged by size: 5213 676 197 160 43 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6289 PsiI TTA'TAA Cuts at: 3859 3859 Size: 6289 PspOMI G'GGCC_C Cuts at: 2690 3026 3409 3530 2690 Size: 336 383 121 5449 Fragments arranged by size: 5449 383 336 121 PstI C_TGCA'G Cuts at: 830 2314 2684 3641 830 Size: 1484 370 957 3478 Fragments arranged by size: 3478 1484 957 370 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6289 PvuII CAG'CTG Cuts at: 2404 3023 2404 Size: 619 5670 SacII CC_GC'GG Cuts at: 2554 2554 Size: 6289 SalI G'TCGA_C Cuts at: 1936 3631 1936 Size: 1695 4594 SanDI GG'GwC_CC Cuts at: 3007 3167 3007 Size: 160 6129 SapI GCTCTTCn'nnn_ Cuts at: 2593 3367 2593 Size: 774 5515 SbfI CC_TGCA'GG Cuts at: 3641 3641 Size: 6289 ScaI AGT'ACT Cuts at: 4537 6249 4537 Size: 1712 4577 SfcI C'TryA_G Cuts at: 826 1045 2206 2310 2653 2680 2818 2865 2938 Size: 219 1161 104 343 27 138 47 73 Cuts at: 2938 3637 4772 5453 5644 826 Size: 699 1135 681 191 1471 Fragments arranged by size: 1471 1161 1135 699 681 343 219 191 138 104 73 47 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6289 SmaI CCC'GGG Cuts at: 1872 3616 1872 Size: 1744 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3189 4393 5294 5556 820 Size: 197 926 1246 1204 901 262 1553 Fragments arranged by size: 1553 1246 1204 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4080 493 Size: 3587 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6289 SphI G_CATG'C Cuts at: 1132 3647 1132 Size: 2515 3774 SspI AAT'ATT Cuts at: 5 52 4213 5 Size: 47 4161 2081 Fragments arranged by size: 4161 2081 47 StuI AGG'CCT Cuts at: 1517 2755 1517 Size: 1238 5051 StyI C'CwwG_G Cuts at: 513 1065 2004 2094 3162 3565 3728 513 Size: 552 939 90 1068 403 163 3074 Fragments arranged by size: 3074 1068 939 552 403 163 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 2888 4316 4333 4486 4671 5116 Size: 1255 450 858 1428 17 153 185 445 Cuts at: 5116 325 Size: 1498 Fragments arranged by size: 1498 1428 1255 858 450 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4535 6247 96 Size: 274 80 33 51 1443 2558 1712 138 Fragments arranged by size: 2558 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2020 2052 6249 6281 2020 Size: 32 4197 32 2028 Fragments arranged by size: 4197 2028 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6289 XbaI T'CTAG_A Cuts at: 3625 3625 Size: 6289 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2513 2783 2513 Size: 270 6019 XhoI C'TCGA_G Cuts at: 1943 3189 1943 Size: 1246 5043 XmaI C'CCGG_G Cuts at: 1870 3614 1870 Size: 1744 4545 XmnI GAAnn'nnTTC Cuts at: 4418 4418 Size: 6289 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4097 276 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI