################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI Aor51HI ApaI AscI AspEI AspI AviII AvrII BalI BamHI BbeI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 719 GGTACC G^GTACC Alw44I 269 GTGCAC G^TGCAC AocI 1400 CCTTAGG CC^T[ACGT]AGG ApaLI 269 GTGCAC G^TGCAC AseI 1360 ATTAAT AT^TAAT AsnI 1360 ATTAAT AT^TAAT Asp718I 719 GGTACC G^GTACC AspHI 269 GTGCAC G[AT]GC[AT]^C AvaI 947 CTCGAG C^[CT]CG[GA]G AvaII 367 GGACC G^G[AT]CC BanI 719 GGTACC G^G[CT][GA]CC BanII 470 GAGCCC G[GA]GC[CT]^C BbrPI 266 CACGTG CAC^GTG BbsI 297 GTCTTC GAAGAC(2/6) BbvCI 170 CCTCAGC CCTCAGC(-5/-2) BcgI 1887 CGATTCATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 518 TGATCA T^GATCA BglII 642 AGATCT A^GATCT BpuAI 297 GTCTTC GAAGAC(2/6) BsaI 2022 GAGACC GGTCTC(1/5) BsaJI 473 CCCAGG C^C[ACGT][ACGT]GG BsaWI 1186 TCCGGT [AT]^CCGG[AT] BsiHKAI 269 GTGCAC G[AT]GC[AT]^C BsoBI 947 CTCGAG C^[CT]CG[GA]G BstAPI 1169 GCAACAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1400 CCTTAGG CC^T[ACGT]AGG DraI 12 TTTAAA TTT^AAA DraII 1847 AGGCCCT [GA]G^G[ACGT]CC[CT] EarI 2019 GAAGAG CTCTTC(1/4) Eco81I 1400 CCTTAGG CC^T[ACGT]AGG EcoO109I 1847 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT22I 935 ATGCAT ATGCA^T FauI 1950 GCGGG CCCGC(4/6) FbaI 518 TGATCA T^GATCA HapII 1187 CCGG C^CGG HpaII 1187 CCGG C^CGG KpnI 719 GGTACC GGTAC^C Ksp632I 2019 GAAGAG CTCTTC(1/4) MslI 1446 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1187 CCGG C^CGG NdeI 1892 CATATG CA^TATG NsiI 935 ATGCAT ATGCA^T NspI 841 ACATGC [GA]CATG^[CT] PaeR7I 947 CTCGAG C^TCGAG PleI 763 GACTC GAGTC(4/5) PmaCI 266 CACGTG CAC^GTG PmlI 266 CACGTG CAC^GTG PshBI 1360 ATTAAT AT^TAAT SpeI 807 ACTAGT A^CTAGT Tsp45I 893 GTGAC ^GT[GC]AC XbaI 1077 TCTAGA T^CTAGA XhoI 947 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AciI 1327 GCGG CCGC(-3/-1) 1950 GCGG AlwI 568 GATCC GGATC(4/5) 1585 GATCC BsaAI 266 CACGTG [CT]AC^GT[GA] 377 CACGTA BseRI 1307 GAGGAG GAGGAG(10/8) 1987 GAGGAG BsgI 305 CTGCAC GTGCAG(16/14) 1973 GTGCAG BspMI 1489 ACCTGC ACCTGC(4/8) 1095 GCAGGT Cac8I 1999 GCCAGC GC[ACGT]^[ACGT]GC 2003 GCAAGC HaeIII 275 GGCC GG^CC 1848 GGCC SfcI 209 CTGTAG C^T[GA][CT]AG 1580 CTGTAG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I BbvI BfaI BmyI BpmI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI