(Circular) (Six-base) MAPSORT of: pFN21AE0499.seq Check: 8977 from: 1 to: 6742 pFN21AE0499 6742 bp With 182 enzymes: SgfI * February 26, 2010 09:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4552 278 Size: 53 83 186 936 3016 2468 Fragments arranged by size: 3016 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4085 1937 Size: 2148 4594 Acc65I G'GTAC_C Cuts at: 2724 4063 2724 Size: 1339 5403 AclI AA'CG_TT Cuts at: 1563 4869 5242 1563 Size: 3306 373 3063 Fragments arranged by size: 3306 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3891 4805 6189 6457 1632 Size: 2259 914 1384 268 1917 Fragments arranged by size: 2259 1917 1384 914 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6545 AflIII A'CryG_T Cuts at: 1203 5641 6618 1203 Size: 4438 977 1327 Fragments arranged by size: 4438 1327 977 AgeI A'CCGG_T Cuts at: 5573 5573 Size: 6742 AhdI GACnn_n'nnGTC Cuts at: 5471 5471 Size: 6742 AleI CACnn'nnGTG Cuts at: 1166 3455 1166 Size: 2289 4453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6742 AlwNI CAG_nnn'CTG Cuts at: 4485 6057 4485 Size: 1572 5170 ApaLI G'TGCA_C Cuts at: 2274 4799 5955 2274 Size: 2525 1156 3061 Fragments arranged by size: 3061 2525 1156 ApoI r'AATT_y Cuts at: 1493 2366 2934 3027 3278 4051 4277 4429 6538 Size: 873 568 93 251 773 226 152 2109 Cuts at: 6538 1493 Size: 1697 Fragments arranged by size: 2109 1697 873 773 568 251 226 152 93 AseI AT'TA_AT Cuts at: 160 1032 3366 5296 160 Size: 872 2334 1930 1606 Fragments arranged by size: 2334 1930 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6742 AvaI C'yCGr_G Cuts at: 1870 1943 2952 4067 1870 Size: 73 1009 1115 4545 Fragments arranged by size: 4545 1115 1009 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3475 3508 3475 Size: 33 6709 BamHI G'GATC_C Cuts at: 4072 4439 4072 Size: 367 6375 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2724 4063 5518 618 Size: 325 217 627 937 1339 1455 1842 Fragments arranged by size: 1842 1455 1339 937 627 325 217 BanII G_rGCy'C Cuts at: 1391 2479 4061 1391 Size: 1088 1582 4072 Fragments arranged by size: 4072 1582 1088 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2295 928 Size: 971 396 5375 Fragments arranged by size: 5375 971 396 BbvCI CC'TCA_GC Cuts at: 2176 2176 Size: 6742 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3881 3915 4933 4967 1903 Size: 34 1944 34 1018 34 3678 Fragments arranged by size: 3678 1944 1018 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4634 5844 4634 Size: 1210 5532 BclI T'GATC_A Cuts at: 1548 2523 1548 Size: 975 5767 BfrBI ATG'CAT Cuts at: 2942 2942 Size: 6742 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5353 136 Size: 107 122 71 631 605 3681 1525 Fragments arranged by size: 3681 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2647 6737 2647 Size: 4090 2652 BlpI GC'TnA_GC Cuts at: 4159 4159 Size: 6742 Bme1580I G_kGCm'C Cuts at: 1790 2278 4039 4803 5959 1790 Size: 488 1761 764 1156 2573 Fragments arranged by size: 2573 1761 1156 764 488 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6742 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2139 2277 4473 Size: 416 53 83 399 24 722 138 2196 Cuts at: 4473 5431 442 Size: 958 1753 Fragments arranged by size: 2196 1753 958 722 416 399 138 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6742 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3699 3699 Size: 6742 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2151 2328 3501 5402 1239 Size: 872 40 177 1173 1901 2579 Fragments arranged by size: 2579 1901 1173 872 177 40 Bpu10I CC'TnA_GC Cuts at: 2176 2176 Size: 6742 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3371 3944 4867 5732 6030 3371 Size: 573 923 865 298 4083 Fragments arranged by size: 4083 923 865 573 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4021 5405 882 Size: 617 2522 1384 2219 Fragments arranged by size: 2522 2219 1384 617 BsaAI yAC'GTr Cuts at: 493 1204 2273 2384 4533 493 Size: 711 1069 111 2149 2702 Fragments arranged by size: 2702 2149 1069 711 111 BsaBI GATnn'nnATC Cuts at: 6736 6736 Size: 6742 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4549 4931 5566 275 Size: 53 83 186 936 3016 382 635 1451 Fragments arranged by size: 3016 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3191 5175 5573 5847 5994 6405 3191 Size: 1984 398 274 147 411 3528 Fragments arranged by size: 3528 1984 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3499 3529 1178 Size: 30 165 30 2096 30 4391 Fragments arranged by size: 4391 2096 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3327 4007 1186 Size: 2141 680 3921 Fragments arranged by size: 3921 2141 680 BseYI C'CCAG_C Cuts at: 5945 6441 5945 Size: 496 6246 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2295 3999 1163 Size: 573 559 1704 3906 Fragments arranged by size: 3906 1704 573 559 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4192 4953 5981 1540 Size: 275 186 2191 761 1028 2301 Fragments arranged by size: 2301 2191 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2278 4803 4888 5959 2278 Size: 2525 85 1071 3061 Fragments arranged by size: 3061 2525 1071 85 BsmI GAATG_Cn' Cuts at: 4253 4346 4253 Size: 93 6649 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2278 2479 4039 4061 4803 4888 5959 Size: 399 488 201 1560 22 742 85 1071 Cuts at: 5959 1391 Size: 2174 Fragments arranged by size: 2174 1560 1071 742 488 399 201 85 22 BspEI T'CCGG_A Cuts at: 6405 6405 Size: 6742 BspHI T'CATG_A Cuts at: 4631 6361 4631 Size: 1730 5012 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3091 3503 4097 844 Size: 2247 412 594 3489 Fragments arranged by size: 3489 2247 594 412 BsrBI CCG'CTC Cuts at: 1394 1823 4629 1394 Size: 429 2806 3507 Fragments arranged by size: 3507 2806 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5237 5411 65 Size: 1183 3989 174 1396 Fragments arranged by size: 3989 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5386 5573 1700 Size: 110 3576 187 2869 Fragments arranged by size: 3576 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6742 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6742 BssSI C'ACGA_G Cuts at: 4802 5814 4802 Size: 1012 5730 BstAPI GCAn_nnn'nTGC Cuts at: 794 3180 794 Size: 2386 4356 BstBI TT'CG_AA Cuts at: 5546 5546 Size: 6742 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6742 BstYI r'GATC_y Cuts at: 1972 2572 2647 3589 4072 4439 4824 4841 6293 Size: 600 75 942 483 367 385 17 1452 Cuts at: 6293 6379 6391 6737 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 942 600 483 385 367 346 86 75 17 12 Bsu36I CC'TnA_GG Cuts at: 3406 3406 Size: 6742 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5251 Fragments arranged by size: 5251 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6003 Fragments arranged by size: 6003 683 56 BtsI GCAGTG_nn' Cuts at: 3240 3819 4258 5052 5072 3240 Size: 579 439 794 20 4910 Fragments arranged by size: 4910 794 579 439 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6742 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6707 DraI TTT'AAA Cuts at: 2019 4046 2019 Size: 2027 4715 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5749 809 Size: 507 4433 1802 Fragments arranged by size: 4433 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4189 5078 6707 Size: 54 1425 50 275 18 2359 889 1629 Cuts at: 6707 8 Size: 43 Fragments arranged by size: 2359 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4189 1537 Size: 275 2377 4090 Fragments arranged by size: 4090 2377 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4019 4672 1353 Size: 2666 653 3423 Fragments arranged by size: 3423 2666 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5329 5703 5849 387 Size: 1064 138 3740 374 146 1280 Fragments arranged by size: 3740 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2151 2328 3501 3891 4805 5402 Size: 393 479 40 177 1173 390 914 597 Cuts at: 5402 6189 6457 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1173 914 787 597 479 393 390 268 177 40 EcoO109I rG'GnC_Cy Cuts at: 3853 3853 Size: 6742 EcoRI G'AATT_C Cuts at: 4051 4429 4051 Size: 378 6364 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3486 3874 3938 3486 Size: 388 64 6290 Fragments arranged by size: 6290 388 64 FspI TGC'GCA Cuts at: 5248 5248 Size: 6742 HaeII r_GCGC'y Cuts at: 1678 5889 1678 Size: 4211 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3574 3606 3679 3711 5383 Size: 32 135 32 2002 32 73 32 1672 Cuts at: 5383 5415 5457 5489 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2002 1672 135 73 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4086 4332 669 Size: 988 281 2148 246 3079 Fragments arranged by size: 3079 2148 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6742 HpaI GTT'AAC Cuts at: 4332 4332 Size: 6742 KpnI G_GTAC'C Cuts at: 2728 4067 2728 Size: 1339 5403 MfeI C'AATT_G Cuts at: 4341 4341 Size: 6742 MluI A'CGCG_T Cuts at: 6618 6618 Size: 6742 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3617 5856 6040 1305 Size: 2312 2239 184 2007 Fragments arranged by size: 2312 2239 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4920 Fragments arranged by size: 4920 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3455 4700 5059 5218 518 Size: 648 2289 1245 359 159 2042 Fragments arranged by size: 2289 2042 1245 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4158 4835 5983 6228 1606 Size: 2552 677 1148 245 2120 Fragments arranged by size: 2552 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5251 Fragments arranged by size: 5251 939 552 NdeI CA'TA_TG Cuts at: 387 3898 387 Size: 3511 3231 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6742 NotI GC'GGCC_GC Cuts at: 4189 4189 Size: 6742 NsiI A_TGCA'T Cuts at: 2944 2944 Size: 6742 NspI r_CATG'y Cuts at: 1132 1229 2850 4100 5645 1132 Size: 97 1621 1250 1545 2229 Fragments arranged by size: 2229 1621 1545 1250 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6742 PciI A'CATG_T Cuts at: 5641 5641 Size: 6742 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6742 PfoI T'CCnGG_A Cuts at: 3517 3819 3517 Size: 302 6440 PmeI GTTT'AAAC Cuts at: 4046 4046 Size: 6742 PmlI CAC'GTG Cuts at: 2273 2273 Size: 6742 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4811 4843 6324 6356 4811 Size: 32 1481 32 5197 Fragments arranged by size: 5197 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6742 PsiI TTA'TAA Cuts at: 4312 4312 Size: 6742 PstI C_TGCA'G Cuts at: 830 4094 830 Size: 3264 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6742 SalI G'TCGA_C Cuts at: 1936 4084 1936 Size: 2148 4594 SbfI CC_TGCA'GG Cuts at: 4094 4094 Size: 6742 ScaI AGT'ACT Cuts at: 4990 6702 4990 Size: 1712 5030 SfcI C'TryA_G Cuts at: 826 1045 2214 3585 4090 5225 5906 6097 826 Size: 219 1169 1371 505 1135 681 191 1471 Fragments arranged by size: 1471 1371 1169 1135 681 505 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6742 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6742 SmaI CCC'GGG Cuts at: 1872 4069 1872 Size: 2197 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2952 3386 3923 4846 5747 6009 Size: 197 926 1009 434 537 923 901 262 Cuts at: 6009 820 Size: 1553 Fragments arranged by size: 1553 1009 926 923 901 537 434 262 197 SnaBI TAC'GTA Cuts at: 493 4533 493 Size: 4040 2702 SpeI A'CTAG_T Cuts at: 152 2812 152 Size: 2660 4082 SphI G_CATG'C Cuts at: 1132 4100 1132 Size: 2968 3774 SspI AAT'ATT Cuts at: 5 52 4666 5 Size: 47 4614 2081 Fragments arranged by size: 4614 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6742 StyI C'CwwG_G Cuts at: 513 1065 2004 4181 513 Size: 552 939 2177 3074 Fragments arranged by size: 3074 2177 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2882 4769 4786 4939 5124 5569 325 Size: 1255 1302 1887 17 153 185 445 1498 Fragments arranged by size: 1887 1498 1302 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3151 4988 6700 Size: 274 80 33 51 1443 1174 1837 1712 Cuts at: 6700 96 Size: 138 Fragments arranged by size: 1837 1712 1443 1174 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3499 3531 6702 6734 3499 Size: 32 3171 32 3507 Fragments arranged by size: 3507 3171 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6742 XbaI T'CTAG_A Cuts at: 3082 4078 3082 Size: 996 5746 XhoI C'TCGA_G Cuts at: 1943 2952 1943 Size: 1009 5733 XmaI C'CCGG_G Cuts at: 1870 4067 1870 Size: 2197 4545 XmnI GAAnn'nnTTC Cuts at: 3828 3918 3971 4871 3828 Size: 90 53 900 5699 Fragments arranged by size: 5699 900 90 53 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4550 276 Size: 53 83 186 936 3016 2468 Fragments arranged by size: 3016 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI