################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HindIII HinfI HpaI KasI KpnI KspI MaeI MaeIII MamI MboI MfeI MflI MluI MroI MslI MunI NarI NciI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 430 GACGTC GACGT^C Aor51HI 234 AGCGCT AGC^GCT AviII 539 TGCGCA TGC^GCA BalI 93 TGGCCA TGG^CCA BanII 144 GAGCCC G[GA]GC[CT]^C BbvCI 589 GCTGAGG CCTCAGC(-5/-2) BpmI 547 CTCCAG CTGGAG(16/14) BsaJI 455 CCCTGG C^C[ACGT][ACGT]GG BsiYI 159 CCAGTACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 159 CCAGTACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 181 GAGAC GTCTC(1/5) BspHI 74 TCATGA T^CATGA BsrFI 598 GCCGGC [GA]^CCGG[CT] Cfr10I 598 GCCGGC [GA]^CCGG[CT] DraIII 45 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 460 GAAGAG CTCTTC(1/4) Eco47III 234 AGCGCT AGC^GCT FauI 530 CCCGC CCCGC(4/6) FspI 539 TGCGCA TGC^GCA HaeII 234 AGCGCT [GA]GCGC^[CT] HapII 599 CCGG C^CGG HgaI 476 GCGTC GACGC(5/10) HincII 433 GTCAAC GT[CT]^[GA]AC HindII 433 GTCAAC GT[CT]^[GA]AC HpaII 599 CCGG C^CGG Ksp632I 460 GAAGAG CTCTTC(1/4) MluNI 93 TGGCCA TGG^CCA MscI 93 TGGCCA TGG^CCA MseI 365 TTAA T^TAA MspI 599 CCGG C^CGG NaeI 598 GCCGGC GCC^GGC NgoMIV 598 GCCGGC G^CCGGC NlaIV 545 GGCTCC GG[ACGT]^[ACGT]CC PflMI 159 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 74 TCATGA T^CATGA Tru9I 365 TTAA T^TAA Van91I 159 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 155 CCAGCCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 325 CGCG CG^CG 358 CGCG AcyI 430 GACGTC G[GA]^CG[CT]C 475 GGCGTC BglI 86 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 586 GCCGCTGAGGC BlpI 36 GCTGAGC GC^T[ACGT]AGC 237 GCTCAGC BmyI 84 GTGCCC G[AGT]GC[ACT]^C 144 GAGCCC Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC 237 GCTCAGC BsaAI 163 TACGTG [CT]AC^GT[GA] 226 TACGTG BsaHI 430 GACGTC G[GA]^CG[CT]C 475 GGCGTC Bsp1286I 84 GTGCCC G[AGT]GC[ACT]^C 144 GAGCCC BstAPI 44 GCACCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 308 GCAACGGCTGC BstF5I 345 CATCC GGATG(2/0) 441 GGATG BstUI 325 CGCG CG^CG 358 CGCG CelII 36 GCTGAGC GC^T[ACGT]AGC 237 GCTCAGC EaeI 93 TGGCCA [CT]^GGCC[GA] 152 CGGCCA FokI 345 CATCC GGATG(9/13) 441 GGATG Hin1I 430 GACGTC G[GA]^CG[CT]C 475 GGCGTC MboII 460 GAAGA GAAGA(8/7) 125 TCTTC MspA1I 276 CCGCTG C[AC]G^C[GT]G 587 CCGCTG MvnI 325 CGCG CG^CG 358 CGCG PstI 372 CTGCAG CTGCA^G 392 CTGCAG SfaNI 492 GATGC GCATC(5/9) 533 GCATC SfcI 372 CTGCAG C^T[GA][CT]AG 392 CTGCAG SmlI 133 CTCAAG C^T[CT][GA]AG 250 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BseRI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI EcoRII Fnu4HI HaeIII HhaI HinP1I HphI ItaI MaeII MnlI MvaI MwoI NlaIII RsaI Sau96I ScrFI TseI TspRI