Restriction map for
pFN21AE3637
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DrdI DsaI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 186 CGCG CG^CG
AhdI 106 GACAAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 539 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 106 GACAAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 569 TGGCCA TGG^CCA
BamHI 119 GGATCC G^GATCC
BanI 783 GGCACC G^G[CT][GA]CC
BbsI 57 GAAGAC GAAGAC(2/6)
BbvCI 631 GCTGAGG CCTCAGC(-5/-2)
BcgI 238 CGAAACCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1451 AGATCT A^GATCT
BpuAI 57 GAAGAC GAAGAC(2/6)
BsaAI 988 TACGTG [CT]AC^GT[GA]
BsaWI 777 ACCGGA [AT]^CCGG[AT]
BseRI 483 CTCCTC GAGGAG(10/8)
BsrBI 619 GAGCGG CCGCTC(-3/-3)
BsrDI 1069 GCAATG GCAATG(2/0)
BstUI 186 CGCG CG^CG
BstXI 560 CCAACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 814 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 309 CGGCCG C^GGCCG
Eam1105I 106 GACAAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1513 CTCTTC CTCTTC(1/4)
EclXI 309 CGGCCG C^GGCCG
Eco52I 309 CGGCCG C^GGCCG
FauI 621 GCGGG CCCGC(4/6)
HgaI 827 GCGTC GACGC(5/10)
HindIII 1411 AAGCTT A^AGCTT
Ksp632I 1513 CTCTTC CTCTTC(1/4)
MaeII 989 ACGT A^CGT
MluNI 569 TGGCCA TGG^CCA
MscI 569 TGGCCA TGG^CCA
MvnI 186 CGCG CG^CG
PflMI 1431 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 869 AGGACCT [GA]G^G[AT]CC[CT]
SapI 1512 GCTCTTC GCTCTTC(1/4)
SexAI 815 ACCTGGT A^CC[AT]GGT
SmaI 902 CCCGGG CCC^GGG
SphI 342 GCATGC GCATG^C
TaqI 224 TCGA T^CGA
Tsp509I 75 AATT ^AATT
TthHB8I 224 TCGA T^CGA
Van91I 1431 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 980 CCATTCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 902 CCCGGG C^CCGGG
XmaI 902 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 987 GTAC GT^AC
1329 GTAC
AocI 499 CCTGAGG CC^T[ACGT]AGG
536 CCTCAGG
Aor51HI 176 AGCGCT AGC^GCT
884 AGCGCT
AvaI 290 CCCGAG C^[CT]CG[GA]G
902 CCCGGG
AvaII 870 GGACC G^G[AT]CC
1479 GGTCC
BsiEI 225 CGACCG CG[GA][CT]^CG
309 CGGCCG
BsmAI 18 GAGAC GTCTC(1/5)
847 GAGAC
BsoBI 290 CCCGAG C^[CT]CG[GA]G
902 CCCGGG
BspMI 494 ACCTGC ACCTGC(4/8)
872 ACCTGC
BstAPI 784 GCACCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
813 GCACCTGGTGC
BstYI 119 GGATCC [GA]^GATC[CT]
1451 AGATCT
Bsu36I 499 CCTGAGG CC^T[ACGT]AGG
536 CCTCAGG
Eco47III 176 AGCGCT AGC^GCT
884 AGCGCT
Eco81I 499 CCTGAGG CC^T[ACGT]AGG
536 CCTCAGG
HinfI 1368 GACTC G^A[ACGT]TC
1531 GAGTC
MflI 119 GGATCC [GA]^GATC[CT]
1451 AGATCT
PleI 1368 GACTC GAGTC(4/5)
1531 GAGTC
RsaI 987 GTAC GT^AC
1329 GTAC
Tsp45I 913 GTGAC ^GT[GC]AC
1533 GTCAC
XhoII 119 GGATCC [GA]^GATC[CT]
1451 AGATCT
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwI ApaI AspHI
BanII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PstI PvuII SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
TspRI