(Circular) (Six-base) MAPSORT of: pFN21AE3637.seq Check: 4939 from: 1 to: 6259 pFN21AE3637 6259 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4069 278 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3602 1937 Size: 1665 4594 Acc65I G'GTAC_C Cuts at: 3580 3580 Size: 6259 AclI AA'CG_TT Cuts at: 1563 4386 4759 1563 Size: 2823 373 3063 Fragments arranged by size: 3063 2823 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3505 4322 5706 5974 1632 Size: 1873 817 1384 268 1917 Fragments arranged by size: 1917 1873 1384 817 268 AfeI AGC'GCT Cuts at: 2183 2891 2183 Size: 708 5551 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6062 AflIII A'CryG_T Cuts at: 1203 5158 6135 1203 Size: 3955 977 1327 Fragments arranged by size: 3955 1327 977 AgeI A'CCGG_T Cuts at: 5090 5090 Size: 6259 AhdI GACnn_n'nnGTC Cuts at: 2116 4988 2116 Size: 2872 3387 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6259 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6259 AlwNI CAG_nnn'CTG Cuts at: 2549 4002 5574 2549 Size: 1453 1572 3234 Fragments arranged by size: 3234 1572 1453 ApaI G_GGCC'C Cuts at: 2017 2428 2556 2747 2017 Size: 411 128 191 5529 Fragments arranged by size: 5529 411 191 128 ApaLI G'TGCA_C Cuts at: 4316 5472 4316 Size: 1156 5103 ApoI r'AATT_y Cuts at: 1493 3568 3794 3946 6055 1493 Size: 2075 226 152 2109 1697 Fragments arranged by size: 2109 2075 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4813 160 Size: 872 3781 1606 Fragments arranged by size: 3781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 AvaI C'yCGr_G Cuts at: 1870 1943 2295 2907 3584 1870 Size: 73 352 612 677 4545 Fragments arranged by size: 4545 677 612 352 73 BamHI G'GATC_C Cuts at: 2124 3589 3956 2124 Size: 1465 367 4427 Fragments arranged by size: 4427 1465 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2788 3580 5035 618 Size: 325 217 627 1001 792 1455 1842 Fragments arranged by size: 1842 1455 1001 792 627 325 217 BanII G_rGCy'C Cuts at: 1391 2017 2428 2463 2489 2556 2589 2747 3036 Size: 626 411 35 26 67 33 158 289 Cuts at: 3036 3192 3303 3519 3578 1391 Size: 156 111 216 59 4072 Fragments arranged by size: 4072 626 411 289 216 158 156 111 67 59 35 33 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2069 928 Size: 971 170 5118 Fragments arranged by size: 5118 971 170 BbvCI CC'TCA_GC Cuts at: 2637 2637 Size: 6259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2232 2266 4450 4484 1903 Size: 34 295 34 2184 34 3678 Fragments arranged by size: 3678 2184 295 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4151 5361 4151 Size: 1210 5049 BclI T'GATC_A Cuts at: 1548 1548 Size: 6259 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4870 136 Size: 107 122 71 631 605 3198 1525 Fragments arranged by size: 3198 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3456 6254 3456 Size: 2798 3461 BlpI GC'TnA_GC Cuts at: 3676 3676 Size: 6259 Bme1580I G_kGCm'C Cuts at: 1790 2017 2428 2556 2747 2968 4320 5476 1790 Size: 227 411 128 191 221 1352 1156 2573 Fragments arranged by size: 2573 1352 1156 411 227 221 191 128 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6259 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2744 2760 3990 Size: 416 53 83 399 24 1327 16 1230 Cuts at: 3990 4948 442 Size: 958 1753 Fragments arranged by size: 1753 1327 1230 958 416 399 83 53 24 16 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2400 2891 3362 4919 1239 Size: 1161 491 471 1557 2579 Fragments arranged by size: 2579 1557 1161 491 471 Bpu10I CC'TnA_GC Cuts at: 2622 2637 2622 Size: 15 6244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4384 5249 5547 4384 Size: 865 298 5096 Fragments arranged by size: 5096 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4922 882 Size: 617 3423 2219 Fragments arranged by size: 3423 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2995 4050 493 Size: 711 1791 1055 2702 Fragments arranged by size: 2702 1791 1055 711 BsaBI GATnn'nnATC Cuts at: 6253 6253 Size: 6259 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4066 4448 5083 275 Size: 53 83 186 936 2533 382 635 1451 Fragments arranged by size: 2533 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2782 4692 5090 5364 5511 5922 2782 Size: 1910 398 274 147 411 3119 Fragments arranged by size: 3119 1910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2888 2918 3415 3445 1178 Size: 30 165 30 1485 30 497 30 3992 Fragments arranged by size: 3992 1485 497 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2479 1186 Size: 1293 4966 BseYI C'CCAG_C Cuts at: 2284 5462 5958 2284 Size: 3178 496 2585 Fragments arranged by size: 3178 2585 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2188 2378 2846 1163 Size: 573 452 190 468 4576 Fragments arranged by size: 4576 573 468 452 190 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2233 2317 3709 4470 5498 1540 Size: 275 186 232 84 1392 761 1028 2301 Fragments arranged by size: 2301 1392 1028 761 275 232 186 84 BsiHKAI G_wGCw'C Cuts at: 2820 3036 3192 3519 4320 4405 5476 2820 Size: 216 156 327 801 85 1071 3603 Fragments arranged by size: 3603 1071 801 327 216 156 85 BsmI GAATG_Cn' Cuts at: 3770 3863 3770 Size: 93 6166 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2428 2463 2489 2556 2589 2747 Size: 399 227 411 35 26 67 33 158 Cuts at: 2747 2820 2968 3036 3192 3303 3519 3578 4320 Size: 73 148 68 156 111 216 59 742 Cuts at: 4320 4405 5476 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 411 399 227 216 158 156 148 111 85 73 68 67 59 35 33 26 BspEI T'CCGG_A Cuts at: 5922 5922 Size: 6259 BspHI T'CATG_A Cuts at: 4148 5878 4148 Size: 1730 4529 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2508 2886 3614 844 Size: 1664 378 728 3489 Fragments arranged by size: 3489 1664 728 378 BsrBI CCG'CTC Cuts at: 1394 1823 2626 4146 1394 Size: 429 803 1520 3507 Fragments arranged by size: 3507 1520 803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3081 4754 4928 65 Size: 1183 1833 1673 174 1396 Fragments arranged by size: 1833 1673 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4903 5090 1700 Size: 110 3093 187 2869 Fragments arranged by size: 3093 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6259 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6259 BssSI C'ACGA_G Cuts at: 4319 5331 4319 Size: 1012 5247 BstAPI GCAn_nnn'nTGC Cuts at: 794 2795 2824 794 Size: 2001 29 4229 Fragments arranged by size: 4229 2001 29 BstBI TT'CG_AA Cuts at: 5063 5063 Size: 6259 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6259 BstXI CCAn_nnnn'nTGG Cuts at: 2572 2572 Size: 6259 BstYI r'GATC_y Cuts at: 1972 2124 3456 3589 3956 4341 4358 5810 5896 Size: 152 1332 133 367 385 17 1452 86 Cuts at: 5896 5908 6254 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1332 385 367 346 152 133 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2505 2542 2505 Size: 37 6222 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4768 Fragments arranged by size: 4768 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3362 487 Size: 683 56 2136 3384 Fragments arranged by size: 3384 2136 683 56 BtsI GCAGTG_nn' Cuts at: 2257 3775 4569 4589 2257 Size: 1518 794 20 3927 Fragments arranged by size: 3927 1518 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6259 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2086 2121 3063 3098 3133 3168 529 Size: 35 1522 35 942 35 35 35 3620 Fragments arranged by size: 3620 1522 942 35 35 35 35 35 DraI TTT'AAA Cuts at: 3563 3563 Size: 6259 DraIII CAC_nnn'GTG Cuts at: 2824 2824 Size: 6259 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5266 809 Size: 507 3950 1802 Fragments arranged by size: 3950 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2314 2574 3293 Size: 54 1425 50 275 18 484 260 719 Cuts at: 3293 3706 4595 6224 8 Size: 413 889 1629 43 Fragments arranged by size: 1629 1425 889 719 484 413 275 260 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2314 3706 1537 Size: 275 502 1392 4090 Fragments arranged by size: 4090 1392 502 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3524 4189 1353 Size: 2171 665 3423 Fragments arranged by size: 3423 2171 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 4846 5220 5366 387 Size: 1064 138 570 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 570 374 146 138 EcoICRI GAG'CTC Cuts at: 3034 3190 3517 3034 Size: 156 327 5776 Fragments arranged by size: 5776 327 156 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2400 2891 3362 3505 4322 4919 5706 Size: 393 768 491 471 143 817 597 787 Cuts at: 5706 5974 1239 Size: 268 1524 Fragments arranged by size: 1524 817 787 768 597 491 471 393 268 143 EcoNI CCTnn'n_nnAGG Cuts at: 2418 2504 2978 2418 Size: 86 474 5699 Fragments arranged by size: 5699 474 86 EcoO109I rG'GnC_Cy Cuts at: 2013 2014 2424 2553 2595 2743 2875 2013 Size: 1 410 129 42 148 132 5397 Fragments arranged by size: 5397 410 148 132 129 42 1 EcoRI G'AATT_C Cuts at: 3568 3946 3568 Size: 378 5881 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2182 3535 2182 Size: 1353 4906 FspI TGC'GCA Cuts at: 4765 4765 Size: 6259 HaeII r_GCGC'y Cuts at: 1678 2185 2285 2893 5406 1678 Size: 507 100 608 2513 2531 Fragments arranged by size: 2531 2513 608 507 100 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2289 2321 4900 Size: 32 135 32 530 32 155 32 2579 Cuts at: 4900 4932 4974 5006 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2579 530 155 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3603 3849 669 Size: 988 281 1665 246 3079 Fragments arranged by size: 3079 1665 988 281 246 HindIII A'AGCT_T Cuts at: 748 3416 748 Size: 2668 3591 HpaI GTT'AAC Cuts at: 3849 3849 Size: 6259 KpnI G_GTAC'C Cuts at: 3584 3584 Size: 6259 MfeI C'AATT_G Cuts at: 3858 3858 Size: 6259 MluI A'CGCG_T Cuts at: 6135 6135 Size: 6259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2589 2919 5373 5557 1305 Size: 1284 330 2454 184 2007 Fragments arranged by size: 2454 2007 1284 330 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2576 10 Size: 54 1425 343 744 3693 Fragments arranged by size: 3693 1425 744 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4217 4576 4735 518 Size: 648 3051 359 159 2042 Fragments arranged by size: 3051 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2368 2377 2476 2674 2863 3328 3675 4352 Size: 762 9 99 198 189 465 347 677 Cuts at: 4352 5500 5745 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 762 677 465 347 245 198 189 99 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4768 Fragments arranged by size: 4768 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6259 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6259 NotI GC'GGCC_GC Cuts at: 3706 3706 Size: 6259 NspI r_CATG'y Cuts at: 1132 1229 2293 2323 2351 2859 3443 3617 5162 Size: 97 1064 30 28 508 584 174 1545 Cuts at: 5162 1132 Size: 2229 Fragments arranged by size: 2229 1545 1064 584 508 174 97 30 28 PasI CC'CwG_GG Cuts at: 1348 2482 1348 Size: 1134 5125 PciI A'CATG_T Cuts at: 5158 5158 Size: 6259 PflMI CCAn_nnn'nTGG Cuts at: 1108 3442 1108 Size: 2334 3925 PmeI GTTT'AAAC Cuts at: 3563 3563 Size: 6259 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4328 4360 5841 5873 4328 Size: 32 1481 32 4714 Fragments arranged by size: 4714 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2875 2875 Size: 6259 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6259 PsiI TTA'TAA Cuts at: 3829 3829 Size: 6259 PspOMI G'GGCC_C Cuts at: 2013 2424 2552 2743 2013 Size: 411 128 191 5529 Fragments arranged by size: 5529 411 191 128 PstI C_TGCA'G Cuts at: 830 2271 2373 3185 3333 3456 3611 830 Size: 1441 102 812 148 123 155 3478 Fragments arranged by size: 3478 1441 812 155 148 123 102 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6259 PvuII CAG'CTG Cuts at: 2368 2476 2674 2863 3328 2368 Size: 108 198 189 465 5299 Fragments arranged by size: 5299 465 198 189 108 SacI G_AGCT'C Cuts at: 3036 3192 3519 3036 Size: 156 327 5776 Fragments arranged by size: 5776 327 156 SalI G'TCGA_C Cuts at: 1936 3601 1936 Size: 1665 4594 SapI GCTCTTCn'nnn_ Cuts at: 3524 3524 Size: 6259 SbfI CC_TGCA'GG Cuts at: 3611 3611 Size: 6259 ScaI AGT'ACT Cuts at: 4507 6219 4507 Size: 1712 4547 SexAI A'CCwGG_T Cuts at: 2820 2820 Size: 6259 SfcI C'TryA_G Cuts at: 826 1045 2267 2369 2656 2845 3181 3329 3452 Size: 219 1222 102 287 189 336 148 123 Cuts at: 3452 3607 4742 5423 5614 826 Size: 155 1135 681 191 1471 Fragments arranged by size: 1471 1222 1135 681 336 287 219 191 189 155 148 123 102 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6259 SmaI CCC'GGG Cuts at: 1872 2909 3586 1872 Size: 1037 677 4545 Fragments arranged by size: 4545 1037 677 SmlI C'TyrA_G Cuts at: 820 1017 1943 4363 5264 5526 820 Size: 197 926 2420 901 262 1553 Fragments arranged by size: 2420 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4050 493 Size: 3557 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6259 SphI G_CATG'C Cuts at: 1132 2351 3617 1132 Size: 1219 1266 3774 Fragments arranged by size: 3774 1266 1219 SspI AAT'ATT Cuts at: 5 52 4183 5 Size: 47 4131 2081 Fragments arranged by size: 4131 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6259 StyI C'CwwG_G Cuts at: 513 1065 2004 2428 2454 2577 3174 3408 3555 Size: 552 939 424 26 123 597 234 147 Cuts at: 3555 3698 513 Size: 143 3074 Fragments arranged by size: 3074 939 597 552 424 234 147 143 123 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2700 3421 4286 4303 4456 4641 5086 Size: 1255 1120 721 865 17 153 185 445 Cuts at: 5086 325 Size: 1498 Fragments arranged by size: 1498 1255 1120 865 721 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3333 4505 6217 Size: 274 80 33 51 1443 1356 1172 1712 Cuts at: 6217 96 Size: 138 Fragments arranged by size: 1712 1443 1356 1172 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6219 6251 6219 Size: 32 6227 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6259 XbaI T'CTAG_A Cuts at: 3595 3595 Size: 6259 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2992 2992 Size: 6259 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6259 XmaI C'CCGG_G Cuts at: 1870 2907 3584 1870 Size: 1037 677 4545 Fragments arranged by size: 4545 1037 677 XmnI GAAnn'nnTTC Cuts at: 4388 4388 Size: 6259 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4067 276 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI