Restriction map for pFN21AE5519
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspHI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BanII       BbeI        
BbrPI       BbvCI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
BstEII      BstPI       BstUI       BstXI       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        EaeI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MamI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NspI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     Van91I      
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          531  GTATAC               GT^[AC][GT]AC
AhdI         1134  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         893  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          973  CCTAAGG              CC^T[ACGT]AGG
AspEI        1134  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1153  GGATCC               G^GATCC
BbvI         1036  GCAGC                GCAGC(8/12)
BcgI          901  GCAAGAACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1328  CTAG                 C^TAG
BpmI          696  CTGGAG               CTGGAG(16/14)
BsaI         1141  GGTCTC               GGTCTC(1/5)
BsmAI        1142  GTCTC                GTCTC(1/5)
BsmI          842  GCATTC               GAATGC(1/-1)
Bst1107I      531  GTATAC               GTA^TAC
BstAPI        892  GCAGTGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         503  TTCGAA               TT^CGAA
BstZ17I       531  GTATAC               GTA^TAC
Bsu36I        973  CCTAAGG              CC^T[ACGT]AGG
DraI          369  TTTAAA               TTT^AAA
DraII           3  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          587  CCATGG               C^C[GA][CT]GG
Eam1105I     1134  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           22  CTCTTC               CTCTTC(1/4)
Eco81I        973  CCTAAGG              CC^T[ACGT]AGG
EcoO109I        3  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         204  GAATTC               G^AATTC
EcoRV         187  GATATC               GAT^ATC
EcoT14I       587  CCATGG               C^C[AT][AT]GG
EcoT22I       840  ATGCAT               ATGCA^T
HindIII        14  AAGCTT               A^AGCTT
Ksp632I        22  CTCTTC               CTCTTC(1/4)
MaeI         1328  CTAG                 C^TAG
MfeI          953  CAATTG               C^AATTG
MslI          387  CATGAAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          953  CAATTG               C^AATTG
MwoI          892  GCAGTGCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          587  CCATGG               C^CATGG
NsiI          840  ATGCAT               ATGCA^T
NspV          503  TTCGAA               TT^CGAA
PpuMI           3  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI         1263  CTGCAG               CTGCA^G
SexAI         352  ACCTGGT              A^CC[AT]GGT
SfuI          503  TTCGAA               TT^CGAA
SmlI          456  CTCAAG               C^T[CT][GA]AG
StyI          587  CCATGG               C^C[AT][AT]GG
TseI         1036  GCAGC                G^C[AT]GC
Tsp45I        988  GTGAC                ^GT[GC]AC
XbaI         1327  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI          383  GTAC                 GT^AC
              950  GTAC                
AseI          313  ATTAAT               AT^TAAT
              550  ATTAAT              
AsnI          313  ATTAAT               AT^TAAT
              550  ATTAAT              
AvaII           4  GGTCC                G^G[AT]CC
              298  GGACC               
BbsI         1258  GAAGAC               GAAGAC(2/6)
               70  GTCTTC              
BciVI         628  GGATAC               GTATCC(6/5)
             1292  GGATAC              
BpuAI        1258  GAAGAC               GAAGAC(2/6)
               70  GTCTTC              
BsaJI         155  CCTGGG               C^C[ACGT][ACGT]GG
              587  CCATGG              
BspHI         177  TCATGA               T^CATGA
              776  TCATGA              
BsrDI         209  CATTGC               GCAATG(2/0)
             1305  CATTGC              
BstF5I       1147  CATCC                GGATG(2/0)
              591  GGATG               
Cac8I         897  GCCTGC               GC[ACGT]^[ACGT]GC
             1237  GCCAGC              
Fnu4HI       1036  GCAGC                GC^[ACGT]GC
             1188  GCCGC               
FokI         1147  CATCC                GGATG(9/13)
              591  GGATG               
HaeIII        165  GGCC                 GG^CC
              257  GGCC                
HphI           60  GGTGA                GGTGA(8/7)
              144  GGTGA               
ItaI         1036  GCAGC                GC^[ACGT]GC
             1188  GCCGC               
PshBI         313  ATTAAT               AT^TAAT
              550  ATTAAT              
RcaI          177  TCATGA               T^CATGA
              776  TCATGA              
RsaI          383  GTAC                 GT^AC
              950  GTAC                
SfaNI         469  GATGC                GCATC(5/9)
              889  GATGC               
SfcI          923  CTACAG               C^T[GA][CT]AG
             1263  CTGCAG              
SspI         1177  AATATT               AAT^ATT
             1332  AATATT              
TaqI          504  TCGA                 T^CGA
             1129  TCGA                
TfiI          432  GATTC                G^A[AT]TC
             1164  GATTC               
TthHB8I       504  TCGA                 T^CGA
             1129  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BsiYI       BslI        BsrI        BssKI       BstNI       
BstYI       Cfr13I      DdeI        DpnII       EcoRII      
HinfI       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        NdeII       NlaIII      
NlaIV       PleI        Sau3AI      Sau96I      ScrFI       
Tru9I       Tsp509I     TspRI       XhoII       
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