################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstEII BstPI BstUI BstXI CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO65I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MamI MluI MluNI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 531 GTATAC GT^[AC][GT]AC AhdI 1134 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 893 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 973 CCTAAGG CC^T[ACGT]AGG AspEI 1134 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1153 GGATCC G^GATCC BbvI 1036 GCAGC GCAGC(8/12) BcgI 901 GCAAGAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1328 CTAG C^TAG BpmI 696 CTGGAG CTGGAG(16/14) BsaI 1141 GGTCTC GGTCTC(1/5) BsmAI 1142 GTCTC GTCTC(1/5) BsmI 842 GCATTC GAATGC(1/-1) Bst1107I 531 GTATAC GTA^TAC BstAPI 892 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 503 TTCGAA TT^CGAA BstZ17I 531 GTATAC GTA^TAC Bsu36I 973 CCTAAGG CC^T[ACGT]AGG DraI 369 TTTAAA TTT^AAA DraII 3 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 587 CCATGG C^C[GA][CT]GG Eam1105I 1134 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 22 CTCTTC CTCTTC(1/4) Eco81I 973 CCTAAGG CC^T[ACGT]AGG EcoO109I 3 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 204 GAATTC G^AATTC EcoRV 187 GATATC GAT^ATC EcoT14I 587 CCATGG C^C[AT][AT]GG EcoT22I 840 ATGCAT ATGCA^T HindIII 14 AAGCTT A^AGCTT Ksp632I 22 CTCTTC CTCTTC(1/4) MaeI 1328 CTAG C^TAG MfeI 953 CAATTG C^AATTG MslI 387 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 953 CAATTG C^AATTG MwoI 892 GCAGTGCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 587 CCATGG C^CATGG NsiI 840 ATGCAT ATGCA^T NspV 503 TTCGAA TT^CGAA PpuMI 3 GGGTCCT [GA]G^G[AT]CC[CT] PstI 1263 CTGCAG CTGCA^G SexAI 352 ACCTGGT A^CC[AT]GGT SfuI 503 TTCGAA TT^CGAA SmlI 456 CTCAAG C^T[CT][GA]AG StyI 587 CCATGG C^C[AT][AT]GG TseI 1036 GCAGC G^C[AT]GC Tsp45I 988 GTGAC ^GT[GC]AC XbaI 1327 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 383 GTAC GT^AC 950 GTAC AseI 313 ATTAAT AT^TAAT 550 ATTAAT AsnI 313 ATTAAT AT^TAAT 550 ATTAAT AvaII 4 GGTCC G^G[AT]CC 298 GGACC BbsI 1258 GAAGAC GAAGAC(2/6) 70 GTCTTC BciVI 628 GGATAC GTATCC(6/5) 1292 GGATAC BpuAI 1258 GAAGAC GAAGAC(2/6) 70 GTCTTC BsaJI 155 CCTGGG C^C[ACGT][ACGT]GG 587 CCATGG BspHI 177 TCATGA T^CATGA 776 TCATGA BsrDI 209 CATTGC GCAATG(2/0) 1305 CATTGC BstF5I 1147 CATCC GGATG(2/0) 591 GGATG Cac8I 897 GCCTGC GC[ACGT]^[ACGT]GC 1237 GCCAGC Fnu4HI 1036 GCAGC GC^[ACGT]GC 1188 GCCGC FokI 1147 CATCC GGATG(9/13) 591 GGATG HaeIII 165 GGCC GG^CC 257 GGCC HphI 60 GGTGA GGTGA(8/7) 144 GGTGA ItaI 1036 GCAGC GC^[ACGT]GC 1188 GCCGC PshBI 313 ATTAAT AT^TAAT 550 ATTAAT RcaI 177 TCATGA T^CATGA 776 TCATGA RsaI 383 GTAC GT^AC 950 GTAC SfaNI 469 GATGC GCATC(5/9) 889 GATGC SfcI 923 CTACAG C^T[GA][CT]AG 1263 CTGCAG SspI 1177 AATATT AAT^ATT 1332 AATATT TaqI 504 TCGA T^CGA 1129 TCGA TfiI 432 GATTC G^A[AT]TC 1164 GATTC TthHB8I 504 TCGA T^CGA 1129 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BsiYI BslI BsrI BssKI BstNI BstYI Cfr13I DdeI DpnII EcoRII HinfI MaeIII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI Tru9I Tsp509I TspRI XhoII