(Circular) (Six-base) MAPSORT of: pFN21AE3948.seq Check: 5450 from: 1 to: 5782 pFN21AE3948 5782 bp With 182 enzymes: SgfI * March 28, 2012 11:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2608 2747 3125 1937 Size: 671 139 378 4594 Fragments arranged by size: 4594 671 378 139 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3081 3909 4282 1563 Size: 1518 828 373 3063 Fragments arranged by size: 3063 1518 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 2732 3845 5229 5497 1632 Size: 467 633 1113 1384 268 1917 Fragments arranged by size: 1917 1384 1113 633 467 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 3525 5097 3525 Size: 1572 4210 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2934 3107 1870 Size: 73 991 173 4545 Fragments arranged by size: 4545 991 173 73 AvrII C'CTAG_G Cuts at: 2551 2551 Size: 5782 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3103 4558 618 Size: 325 217 627 1316 1455 1842 Fragments arranged by size: 1842 1455 1316 627 325 217 BanII G_rGCy'C Cuts at: 1391 2023 3101 1391 Size: 632 1078 4072 Fragments arranged by size: 4072 1078 632 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2912 928 Size: 971 1013 3798 Fragments arranged by size: 3798 1013 971 BbvCI CC'TCA_GC Cuts at: 2582 2582 Size: 5782 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4393 136 Size: 107 122 71 631 605 2721 1525 Fragments arranged by size: 2721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2203 5777 2203 Size: 3574 2208 BlpI GC'TnA_GC Cuts at: 2010 2562 3199 2010 Size: 552 637 4593 Fragments arranged by size: 4593 637 552 Bme1580I G_kGCm'C Cuts at: 1790 2918 3843 4999 1790 Size: 1128 925 1156 2573 Fragments arranged by size: 2573 1156 1128 925 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2127 2127 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2414 2613 2657 4442 1239 Size: 959 216 199 44 1785 2579 Fragments arranged by size: 2579 1785 959 216 199 44 Bpu10I CC'TnA_GC Cuts at: 2582 2652 2582 Size: 70 5712 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2434 3907 4772 5070 2434 Size: 1473 865 298 3146 Fragments arranged by size: 3146 1473 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2098 2330 4445 882 Size: 617 599 232 2115 2219 Fragments arranged by size: 2219 2115 617 599 232 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2674 2704 1178 Size: 30 165 30 1271 30 4256 Fragments arranged by size: 4256 1271 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3019 1186 Size: 1833 3949 BseYI C'CCAG_C Cuts at: 4985 5481 4985 Size: 496 5286 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 2643 3843 3928 4999 2016 Size: 627 1200 85 1071 2799 Fragments arranged by size: 2799 1200 1071 627 85 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2023 2643 2918 3101 3843 3928 Size: 399 226 7 620 275 183 742 85 Cuts at: 3928 4999 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 620 399 275 226 183 85 7 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3137 844 Size: 2293 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2111 3669 1394 Size: 429 288 1558 3507 Fragments arranged by size: 3507 1558 429 288 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 2530 2860 96 Size: 2434 330 3018 Fragments arranged by size: 3018 2434 330 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstYI r'GATC_y Cuts at: 1972 2203 3112 3479 3864 3881 5333 5419 5431 Size: 231 909 367 385 17 1452 86 12 Cuts at: 5431 5777 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 909 385 367 346 231 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4291 Fragments arranged by size: 4291 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 2106 2576 3298 4092 4112 2106 Size: 470 722 794 20 3776 Fragments arranged by size: 3776 794 722 470 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 3229 4118 Size: 54 1425 50 275 18 213 1186 889 Cuts at: 4118 5747 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1186 889 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 2415 3712 1353 Size: 765 297 1297 3423 Fragments arranged by size: 3423 1297 765 297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2198 2414 2613 2657 2732 3845 Size: 393 467 99 216 199 44 75 1113 Cuts at: 3845 4442 5229 5497 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1113 787 597 467 393 268 216 199 99 75 44 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 4929 1678 Size: 3251 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2861 2893 4423 Size: 32 135 32 470 32 787 32 1530 Cuts at: 4423 4455 4497 4529 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1530 787 470 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2609 3126 3372 669 Size: 988 281 671 517 246 3079 Fragments arranged by size: 3079 988 671 517 281 246 HindIII A'AGCT_T Cuts at: 748 3015 748 Size: 2267 3515 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3062 4896 5080 1305 Size: 1757 1834 184 2007 Fragments arranged by size: 2007 1834 1757 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3740 4099 4258 518 Size: 648 2574 359 159 2042 Fragments arranged by size: 2574 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2033 2457 2985 3198 3875 5023 5268 1606 Size: 427 424 528 213 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 528 427 424 245 213 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4291 Fragments arranged by size: 4291 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2123 3140 4685 1132 Size: 97 894 1017 1545 2229 Fragments arranged by size: 2229 1545 1017 894 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 3134 830 Size: 2304 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2033 2033 Size: 5782 SalI G'TCGA_C Cuts at: 1936 2607 3124 1936 Size: 671 517 4594 Fragments arranged by size: 4594 671 517 SapI GCTCTTCn'nnn_ Cuts at: 2118 2118 Size: 5782 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 3130 4265 4946 5137 826 Size: 219 2085 1135 681 191 1471 Fragments arranged by size: 2085 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 2936 3109 1872 Size: 1064 173 4545 Fragments arranged by size: 4545 1064 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 2413 3886 4787 5049 820 Size: 197 926 470 1473 901 262 1553 Fragments arranged by size: 1553 1473 926 901 470 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 2432 3706 5 Size: 47 2380 1274 2081 Fragments arranged by size: 2380 2081 1274 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2004 2551 3221 513 Size: 552 939 547 670 3074 Fragments arranged by size: 3074 939 670 552 547 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2168 2753 3809 3826 3979 4164 4609 Size: 1255 588 585 1056 17 153 185 445 Cuts at: 4609 325 Size: 1498 Fragments arranged by size: 1498 1255 1056 588 585 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2530 2860 3046 Size: 274 80 33 51 1443 553 330 186 Cuts at: 3046 4028 5740 96 Size: 982 1712 138 Fragments arranged by size: 1712 1443 982 553 330 274 186 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2674 2706 3019 3051 5742 5774 2674 Size: 32 313 32 2691 32 2682 Fragments arranged by size: 2691 2682 313 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 2231 3118 2231 Size: 887 4895 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2068 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 2934 3107 1870 Size: 1064 173 4545 Fragments arranged by size: 4545 1064 173 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI