(Circular) (Six-base) MAPSORT of: pFN21AE1089.seq Check: 6628 from: 1 to: 6073 pFN21AE1089 6073 bp With 182 enzymes: SgfI * October 14, 2009 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3883 278 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2679 3315 3416 1937 Size: 742 636 101 4594 Fragments arranged by size: 4594 742 636 101 Acc65I G'GTAC_C Cuts at: 3394 3394 Size: 6073 AclI AA'CG_TT Cuts at: 1563 4200 4573 1563 Size: 2637 373 3063 Fragments arranged by size: 3063 2637 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4136 5520 5788 1632 Size: 2504 1384 268 1917 Fragments arranged by size: 2504 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5876 AflIII A'CryG_T Cuts at: 1203 4972 5949 1203 Size: 3769 977 1327 Fragments arranged by size: 3769 1327 977 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 3268 4802 3268 Size: 1534 4539 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6073 AlwNI CAG_nnn'CTG Cuts at: 3816 5388 3816 Size: 1572 4501 ApaLI G'TGCA_C Cuts at: 4130 5286 4130 Size: 1156 4917 ApoI r'AATT_y Cuts at: 1493 2312 2552 3382 3608 3760 5869 1493 Size: 819 240 830 226 152 2109 1697 Fragments arranged by size: 2109 1697 830 819 240 226 152 AseI AT'TA_AT Cuts at: 160 1032 4627 160 Size: 872 3595 1606 Fragments arranged by size: 3595 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 3154 3398 1870 Size: 73 1211 244 4545 Fragments arranged by size: 4545 1211 244 73 BamHI G'GATC_C Cuts at: 2046 3079 3403 3770 2046 Size: 1033 324 367 4349 Fragments arranged by size: 4349 1033 367 324 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2459 3394 4849 618 Size: 325 217 627 672 935 1455 1842 Fragments arranged by size: 1842 1455 935 672 627 325 217 BanII G_rGCy'C Cuts at: 1391 3392 1391 Size: 2001 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 2216 928 Size: 971 220 97 4785 Fragments arranged by size: 4785 971 220 97 BbvCI CC'TCA_GC Cuts at: 3345 3345 Size: 6073 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4264 4298 1903 Size: 34 2327 34 3678 Fragments arranged by size: 3678 2327 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3965 5175 3965 Size: 1210 4863 BclI T'GATC_A Cuts at: 1548 1548 Size: 6073 BfrBI ATG'CAT Cuts at: 2509 2787 3032 2509 Size: 278 245 5550 Fragments arranged by size: 5550 278 245 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4684 136 Size: 107 122 71 631 605 3012 1525 Fragments arranged by size: 3012 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6068 6068 Size: 6073 BlpI GC'TnA_GC Cuts at: 3160 3490 3160 Size: 330 5743 Bme1580I G_kGCm'C Cuts at: 1790 4134 5290 1790 Size: 2344 1156 2573 Fragments arranged by size: 2573 2344 1156 BmgBI CAC'GTC Cuts at: 1318 2645 1318 Size: 1327 4746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3328 3804 4762 Size: 416 53 83 399 24 1911 476 958 Cuts at: 4762 442 Size: 1753 Fragments arranged by size: 1911 1753 958 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4733 1239 Size: 3494 2579 Bpu10I CC'TnA_GC Cuts at: 3345 3345 Size: 6073 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4198 5063 5361 4198 Size: 865 298 4910 Fragments arranged by size: 4910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2170 4736 882 Size: 617 671 2566 2219 Fragments arranged by size: 2566 2219 671 617 BsaAI yAC'GTr Cuts at: 493 1204 3327 3864 493 Size: 711 2123 537 2702 Fragments arranged by size: 2702 2123 711 537 BsaBI GATnn'nnATC Cuts at: 6067 6067 Size: 6073 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3880 4262 4897 275 Size: 53 83 186 936 2347 382 635 1451 Fragments arranged by size: 2347 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2337 2374 4506 4904 5178 5325 5736 2337 Size: 37 2132 398 274 147 411 2674 Fragments arranged by size: 2674 2132 411 398 274 147 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2181 2211 2700 2730 3094 Size: 30 165 30 778 30 489 30 364 Cuts at: 3094 3124 1178 Size: 30 4127 Fragments arranged by size: 4127 778 489 364 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6073 BseYI C'CCAG_C Cuts at: 5276 5772 5276 Size: 496 5577 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5500 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3523 4284 5312 1540 Size: 275 186 1522 761 1028 2301 Fragments arranged by size: 2301 1522 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2063 2877 4134 4219 5290 2063 Size: 814 1257 85 1071 2846 Fragments arranged by size: 2846 1257 1071 814 85 BsmI GAATG_Cn' Cuts at: 3584 3677 3584 Size: 93 5980 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2877 3392 4134 4219 5290 1391 Size: 399 273 814 515 742 85 1071 2174 Fragments arranged by size: 2174 1071 814 742 515 399 273 85 BspEI T'CCGG_A Cuts at: 2374 5736 2374 Size: 3362 2711 BspHI T'CATG_A Cuts at: 3962 5692 3962 Size: 1730 4343 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2787 3428 844 Size: 1943 641 3489 Fragments arranged by size: 3489 1943 641 BsrBI CCG'CTC Cuts at: 1394 1823 3960 1394 Size: 429 2137 3507 Fragments arranged by size: 3507 2137 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3168 4568 4742 65 Size: 1183 1920 1400 174 1396 Fragments arranged by size: 1920 1400 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4717 4904 1700 Size: 110 2907 187 2869 Fragments arranged by size: 2907 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2469 96 Size: 2373 3700 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6073 BssSI C'ACGA_G Cuts at: 4133 5145 4133 Size: 1012 5061 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6073 BstBI TT'CG_AA Cuts at: 4877 4877 Size: 6073 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6073 BstXI CCAn_nnnn'nTGG Cuts at: 2140 2140 Size: 6073 BstYI r'GATC_y Cuts at: 1972 2046 3079 3403 3770 4155 4172 5624 5710 Size: 74 1033 324 367 385 17 1452 86 Cuts at: 5710 5722 6068 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1033 385 367 346 324 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 2919 2919 Size: 6073 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5521 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5334 Fragments arranged by size: 5334 683 56 BtsI GCAGTG_nn' Cuts at: 3589 4383 4403 3589 Size: 794 20 5259 Fragments arranged by size: 5259 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6038 DraI TTT'AAA Cuts at: 2317 3333 3377 2317 Size: 1016 44 5013 Fragments arranged by size: 5013 1016 44 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5080 809 Size: 507 3764 1802 Fragments arranged by size: 3764 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3141 3520 4409 Size: 54 1425 50 275 18 1311 379 889 Cuts at: 4409 6038 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1311 889 379 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3520 1537 Size: 275 1708 4090 Fragments arranged by size: 4090 1708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2738 4003 1353 Size: 1385 1265 3423 Fragments arranged by size: 3423 1385 1265 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4660 5034 5180 387 Size: 1064 138 3071 374 146 1280 Fragments arranged by size: 3071 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4136 4733 5520 5788 1239 Size: 393 2504 597 787 268 1524 Fragments arranged by size: 2504 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2918 2918 Size: 6073 EcoO109I rG'GnC_Cy Cuts at: 2321 2321 Size: 6073 EcoRI G'AATT_C Cuts at: 3382 3760 3382 Size: 378 5695 EcoRV GAT'ATC Cuts at: 2218 2218 Size: 6073 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2123 2583 3256 2123 Size: 460 673 4940 Fragments arranged by size: 4940 673 460 FspI TGC'GCA Cuts at: 4579 4579 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 5220 1678 Size: 3542 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 3254 3286 4714 Size: 32 135 32 427 32 1223 32 1428 Cuts at: 4714 4746 4788 4820 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1428 1223 427 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3417 3663 669 Size: 988 281 1479 246 3079 Fragments arranged by size: 3079 1479 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6073 HpaI GTT'AAC Cuts at: 3663 3663 Size: 6073 KpnI G_GTAC'C Cuts at: 3398 3398 Size: 6073 MfeI C'AATT_G Cuts at: 3672 3672 Size: 6073 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3137 5187 5371 1305 Size: 1832 2050 184 2007 Fragments arranged by size: 2050 2007 1832 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3143 10 Size: 54 1425 343 1311 2940 Fragments arranged by size: 2940 1425 1311 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 4031 4390 4549 518 Size: 648 1257 1608 359 159 2042 Fragments arranged by size: 2042 1608 1257 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3289 3489 4166 5314 5559 1606 Size: 1683 200 677 1148 245 2120 Fragments arranged by size: 2120 1683 1148 677 245 200 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5521 NdeI CA'TA_TG Cuts at: 387 387 Size: 6073 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6073 NotI GC'GGCC_GC Cuts at: 3520 3520 Size: 6073 NsiI A_TGCA'T Cuts at: 2511 2789 3034 2511 Size: 278 245 5550 Fragments arranged by size: 5550 278 245 NspI r_CATG'y Cuts at: 1132 1229 3431 4976 1132 Size: 97 2202 1545 2229 Fragments arranged by size: 2229 2202 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6073 PciI A'CATG_T Cuts at: 4972 4972 Size: 6073 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 1108 Size: 1149 4924 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5655 5687 4142 Size: 32 1481 32 4528 Fragments arranged by size: 4528 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2321 2321 Size: 6073 PshAI GACnn'nnGTC Cuts at: 1613 2089 1613 Size: 476 5597 PsiI TTA'TAA Cuts at: 3643 3643 Size: 6073 PstI C_TGCA'G Cuts at: 830 3425 830 Size: 2595 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6073 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SbfI CC_TGCA'GG Cuts at: 3425 3425 Size: 6073 ScaI AGT'ACT Cuts at: 4321 6033 4321 Size: 1712 4361 SfcI C'TryA_G Cuts at: 826 1045 3421 4556 5237 5428 826 Size: 219 2376 1135 681 191 1471 Fragments arranged by size: 2376 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 3156 3400 1872 Size: 1284 244 4545 Fragments arranged by size: 4545 1284 244 SmlI C'TyrA_G Cuts at: 820 1017 1943 4177 5078 5340 820 Size: 197 926 2234 901 262 1553 Fragments arranged by size: 2234 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3327 3864 493 Size: 2834 537 2702 Fragments arranged by size: 2834 2702 537 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3431 1132 Size: 2299 3774 SspI AAT'ATT Cuts at: 5 52 3997 5 Size: 47 3945 2081 Fragments arranged by size: 3945 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6073 StyI C'CwwG_G Cuts at: 513 1065 3512 513 Size: 552 2447 3074 Fragments arranged by size: 3074 2447 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3279 4100 4117 4270 4455 4900 325 Size: 1255 1699 821 17 153 185 445 1498 Fragments arranged by size: 1699 1498 1255 821 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2469 2702 4319 Size: 274 80 33 51 1443 492 233 1617 Cuts at: 4319 6031 96 Size: 1712 138 Fragments arranged by size: 1712 1617 1443 492 274 233 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3094 3126 6033 6065 3094 Size: 32 2907 32 3102 Fragments arranged by size: 3102 2907 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6073 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6073 XmaI C'CCGG_G Cuts at: 1870 3154 3398 1870 Size: 1284 244 4545 Fragments arranged by size: 4545 1284 244 XmnI GAAnn'nnTTC Cuts at: 4202 4202 Size: 6073 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3881 276 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI