Restriction map for pFN21AE1089
 (Linear) (Six-base) MAPSORT of: KIEE1089.seq  Check: 9010  from: 1  to: 1368



KIEE1089.orf

 With 182 enzymes: SgfI * 

                        October 13, 2009 15:05  ..

AccI GT'mk_AC

Cuts at:      0     674    1310    1368 
   Size:        674     636      58

  Fragments arranged by size: 

                 674     636      58
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1263    1368 
   Size:       1263     105
 
ApoI r'AATT_y

Cuts at:      0     307     547    1368 
   Size:        307     240     821

  Fragments arranged by size: 

                 821     307     240
 
AvaI C'yCGr_G

Cuts at:      0    1149    1368 
   Size:       1149     219
 
BamHI G'GATC_C

Cuts at:      0      41    1074    1368 
   Size:         41    1033     294

  Fragments arranged by size: 

                1033     294      41
 
BanI G'GyrC_C

Cuts at:      0     454    1368 
   Size:        454     914
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     114     211    1368 
   Size:        114      97    1157

  Fragments arranged by size: 

                1157     114      97
 
BbvCI CC'TCA_GC

Cuts at:      0    1340    1368 
   Size:       1340      28
 
BfrBI ATG'CAT

Cuts at:      0     504     782    1027    1368 
   Size:        504     278     245     341

  Fragments arranged by size: 

                 504     341     278     245
 
BlpI GC'TnA_GC

Cuts at:      0    1155    1368 
   Size:       1155     213
 
BmgBI CAC'GTC

Cuts at:      0     640    1368 
   Size:        640     728
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1323    1368 
   Size:       1323      45
 
Bpu10I CC'TnA_GC

Cuts at:      0    1340    1368 
   Size:       1340      28
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     165    1368 
   Size:        165    1203
 
BsaAI yAC'GTr

Cuts at:      0    1322    1368 
   Size:       1322      46
 
BsaWI w'CCGG_w

Cuts at:      0     332     369    1368 
   Size:        332      37     999

  Fragments arranged by size: 

                 999     332      37
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     176     206     695     725    1089    1119    1368 
   Size:        176      30     489      30     364      30     249

  Fragments arranged by size: 

                 489     364     249     176      30      30      30
 
BsiHKAI G_wGCw'C

Cuts at:      0      58     872    1368 
   Size:         58     814     496

  Fragments arranged by size: 

                 814     496      58
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     872    1368 
   Size:         58     814     496

  Fragments arranged by size: 

                 814     496      58
 
BspEI T'CCGG_A

Cuts at:      0     369    1368 
   Size:        369     999
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     782    1368 
   Size:        782     586
 
BsrDI GCAATG_nn'

Cuts at:      0    1163    1368 
   Size:       1163     205
 
BsrGI T'GTAC_A

Cuts at:      0     464    1368 
   Size:        464     904
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     135    1368 
   Size:        135    1233
 
BstYI r'GATC_y

Cuts at:      0      41    1074    1368 
   Size:         41    1033     294

  Fragments arranged by size: 

                1033     294      41
 
Bsu36I CC'TnA_GG

Cuts at:      0     914    1368 
   Size:        914     454
 
DraI TTT'AAA

Cuts at:      0     312    1328    1368 
   Size:        312    1016      40

  Fragments arranged by size: 

                1016     312      40
 
EaeI y'GGCC_r

Cuts at:      0    1136    1368 
   Size:       1136     232
 
EarI CTCTTCn'nnn_

Cuts at:      0     733    1368 
   Size:        733     635
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     913    1368 
   Size:        913     455
 
EcoO109I rG'GnC_Cy

Cuts at:      0     316    1368 
   Size:        316    1052
 
EcoRV GAT'ATC

Cuts at:      0     213    1368 
   Size:        213    1155
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     118     578    1251    1368 
   Size:        118     460     673     117

  Fragments arranged by size: 

                 673     460     118     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26    1249    1281    1368 
   Size:         26    1223      32      87

  Fragments arranged by size: 

                1223      87      32      26
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1132    1368 
   Size:       1132     236
 
MscI TGG'CCA

Cuts at:      0    1138    1368 
   Size:       1138     230
 
MslI CAynn'nnrTG

Cuts at:      0     418    1368 
   Size:        418     950
 
MspA1I CmG'CkG

Cuts at:      0    1284    1368 
   Size:       1284      84
 
NsiI A_TGCA'T

Cuts at:      0     506     784    1029    1368 
   Size:        506     278     245     339

  Fragments arranged by size: 

                 506     339     278     245
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     252    1368 
   Size:        252    1116
 
PpuMI rG'GwC_Cy

Cuts at:      0     316    1368 
   Size:        316    1052
 
PshAI GACnn'nnGTC

Cuts at:      0      84    1368 
   Size:         84    1284
 
SmaI CCC'GGG

Cuts at:      0    1151    1368 
   Size:       1151     217
 
SnaBI TAC'GTA

Cuts at:      0    1322    1368 
   Size:       1322      46
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1274    1368 
   Size:       1274      94
 
TatI w'GTAC_w

Cuts at:      0     464     697    1368 
   Size:        464     233     671

  Fragments arranged by size: 

                 671     464     233
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1089    1121    1368 
   Size:       1089      32     247

  Fragments arranged by size: 

                1089     247      32
 
XmaI C'CCGG_G

Cuts at:      0    1149    1368 
   Size:       1149     219
 

 Enzymes that do cut:

     AccI     AhdI     ApoI     AvaI    BamHI     BanI     BbsI    BbvCI
    BfrBI     BlpI    BmgBI     BmrI   Bpu10I     BsaI    BsaAI    BsaWI
    BsaXI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrDI    BsrGI    BstXI
    BstYI   Bsu36I     DraI     EaeI     EarI    EcoNI EcoO109I    EcoRV
     FalI    Hin4I     MmeI     MscI     MslI   MspA1I     NsiI    PflMI
    PpuMI    PshAI     SmaI    SnaBI    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BanII     BbeI     BcgI    BciVI
     BclI     BglI    BglII Bme1580I     BmtI     BplI     BpmI    BpuEI
    BsaBI    BsaHI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoRI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI
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