################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BclI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccII 858 CGCG CG^CG AclI 688 AACGTT AA^CGTT AfaI 116 GTAC GT^AC AflII 1282 CTTAAG C^TTAAG AlwNI 29 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 661 ATTAAT AT^TAAT AsnI 661 ATTAAT AT^TAAT AspHI 1582 GAGCAC G[AT]GC[AT]^C AspI 1501 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 3 GTCTTC GAAGAC(2/6) BcgI 1352 GCAATTCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 232 GGATAC GTATCC(6/5) BcnI 888 CCGGG CC^[GC]GG BfrI 1282 CTTAAG C^TTAAG BglI 1366 GCCCTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1000 GCTAAGC GC^T[ACGT]AGC BmyI 1582 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 1000 GCTAAGC GC^T[ACGT]AGC BpuAI 3 GTCTTC GAAGAC(2/6) BsaI 976 GAGACC GGTCTC(1/5) BsiHKAI 1582 GAGCAC G[AT]GC[AT]^C Bsp1286I 1582 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 115 TGTACA T^GTACA BspHI 1289 TCATGA T^CATGA BsrGI 115 TGTACA T^GTACA BstUI 858 CGCG CG^CG Cac8I 159 GCTAGC GC[ACGT]^[ACGT]GC CelII 1000 GCTAAGC GC^T[ACGT]AGC CfoI 859 GCGC GCG^C DraI 594 TTTAAA TTT^AAA DsaI 1040 CCATGG C^C[GA][CT]GG EarI 752 GAAGAG CTCTTC(1/4) FauI 1428 GCGGG CCCGC(4/6) HapII 888 CCGG C^CGG HhaI 859 GCGC GCG^C HinP1I 859 GCGC G^CGC HpaII 888 CCGG C^CGG Ksp632I 752 GAAGAG CTCTTC(1/4) MaeIII 505 GTGAC ^GT[ACGT]AC MspA1I 220 CAGCTG C[AC]G^C[GT]G MspI 888 CCGG C^CGG MvnI 858 CGCG CG^CG NciI 888 CCGGG CC^[GC]GG NcoI 1040 CCATGG C^CATGG NdeI 722 CATATG CA^TATG NheI 159 GCTAGC G^CTAGC NspI 818 ACATGC [GA]CATG^[CT] PflFI 1501 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 612 GACTC GAGTC(4/5) PshBI 661 ATTAAT AT^TAAT Psp1406I 688 AACGTT AA^CGTT PstI 447 CTGCAG CTGCA^G PvuII 220 CAGCTG CAG^CTG RcaI 1289 TCATGA T^CATGA RsaI 116 GTAC GT^AC SapI 752 GAAGAGC GCTCTTC(1/4) SexAI 1502 ACCTGGT A^CC[AT]GGT SfcI 447 CTGCAG C^T[GA][CT]AG SmlI 1282 CTTAAG C^T[CT][GA]AG SspBI 115 TGTACA T^GTACA StuI 1600 AGGCCT AGG^CCT Tsp45I 505 GTGAC ^GT[GC]AC Tth111I 1501 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BamHI 490 GGATCC G^GATCC 1483 GGATCC BpmI 479 CTGGAG CTGGAG(16/14) 1070 CTGGAG BseRI 970 GAGGAG GAGGAG(10/8) 973 GAGGAG BsmFI 1235 GGGAC GGGAC(10/14) 1507 GTCCC BstAPI 193 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 813 GCAAGACATGC BstYI 490 GGATCC [GA]^GATC[CT] 1483 GGATCC EcoRI 935 GAATTC G^AATTC 1293 GAATTC HphI 678 GGTGA GGTGA(8/7) 1333 TCACC MflI 490 GGATCC [GA]^GATC[CT] 1483 GGATCC MslI 141 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 705 CATGAGAATG XhoII 490 GGATCC [GA]^GATC[CT] 1483 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BbvI BfaI BsaJI BsiYI BslI BsmAI BsmI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI ItaI MaeI MaeII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp509I TspRI