################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII Cfr10I CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AocI 160 CCTGAGG CC^T[ACGT]AGG AspHI 403 GAGCTC G[AT]GC[AT]^C BanII 403 GAGCTC G[GA]GC[CT]^C BbvCI 91 GCTGAGG CCTCAGC(-5/-2) BmyI 403 GAGCTC G[AGT]GC[ACT]^C BpmI 460 CTGGAG CTGGAG(16/14) BsaI 376 GAGACC GGTCTC(1/5) BsaJI 113 CCAAGG C^C[ACGT][ACGT]GG BsiEI 435 CGGCCG CG[GA][CT]^CG BsiHKAI 403 GAGCTC G[AT]GC[AT]^C Bsp1286I 403 GAGCTC G[AGT]GC[ACT]^C BspDI 181 ATCGAT AT^CGAT BstXI 113 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 160 CCTGAGG CC^T[ACGT]AGG Cac8I 363 GCAGGC GC[ACGT]^[ACGT]GC CfoI 89 GCGC GCG^C Cfr13I 144 GGGCC G^G[ACGT]CC ClaI 181 ATCGAT AT^CGAT DpnII 180 GATC ^GATC EaeI 435 CGGCCG [CT]^GGCC[GA] EagI 435 CGGCCG C^GGCCG EclXI 435 CGGCCG C^GGCCG Eco52I 435 CGGCCG C^GGCCG Eco81I 160 CCTGAGG CC^T[ACGT]AGG EcoT14I 113 CCAAGG C^C[AT][AT]GG HaeII 88 GGCGCT [GA]GCGC^[CT] HhaI 89 GCGC GCG^C HinP1I 89 GCGC G^CGC HphI 129 GGTGA GGTGA(8/7) MboI 180 GATC ^GATC MslI 75 CATCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 101 GCTGCATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 180 GATC ^GATC NspI 305 GCATGT [GA]CATG^[CT] PvuII 31 CAGCTG CAG^CTG SacI 403 GAGCTC GAGCT^C SapI 40 GAAGAGC GCTCTTC(1/4) Sau3AI 180 GATC ^GATC Sau96I 144 GGGCC G^G[ACGT]CC StyI 113 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### BsmAI 376 GAGAC GTCTC(1/5) 419 GAGAC EarI 298 CTCTTC CTCTTC(1/4) 40 GAAGAG HaeIII 145 GGCC GG^CC 436 GGCC Ksp632I 298 CTCTTC CTCTTC(1/4) 40 GAAGAG MspA1I 31 CAGCTG C[AC]G^C[GT]G 204 CAGCGG PstI 169 CTGCAG CTGCA^G 361 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI BbvI BseRI BspHI BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI ItaI MboII MnlI MvaI NlaIII RcaI ScrFI SfaNI SfcI TaqI TseI TthHB8I