(Circular) (Six-base) MAPSORT of: pFN21AB6512.seq Check: 8618 from: 1 to: 5857 pFN21AB6512 5857 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2065 3006 3200 1937 Size: 128 941 194 4594 Fragments arranged by size: 4594 941 194 128 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2523 2874 3920 5304 5572 1632 Size: 891 351 1046 1384 268 1917 Fragments arranged by size: 1917 1384 1046 891 351 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 4756 5733 1203 Size: 3553 977 1327 Fragments arranged by size: 3553 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 2434 2824 4586 2434 Size: 390 1762 3705 Fragments arranged by size: 3705 1762 390 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5857 AlwNI CAG_nnn'CTG Cuts at: 2181 2438 3600 5172 2181 Size: 257 1162 1572 2866 Fragments arranged by size: 2866 1572 1162 257 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 3166 3392 3544 5653 1493 Size: 1673 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 3182 1870 Size: 73 1239 4545 Fragments arranged by size: 4545 1239 73 BamHI G'GATC_C Cuts at: 2306 2867 3187 3554 2306 Size: 561 320 367 4609 Fragments arranged by size: 4609 561 367 320 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3178 4633 618 Size: 325 217 627 1391 1455 1842 Fragments arranged by size: 1842 1455 1391 627 325 217 BanII G_rGCy'C Cuts at: 1391 2803 3085 3176 1391 Size: 1412 282 91 4072 Fragments arranged by size: 4072 1412 282 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2962 928 Size: 971 1063 3823 Fragments arranged by size: 3823 1063 971 BbvCI CC'TCA_GC Cuts at: 2742 3102 2742 Size: 360 5497 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 1548 Size: 5857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4468 136 Size: 107 122 71 631 605 2796 1525 Fragments arranged by size: 2796 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5852 5852 Size: 5857 BlpI GC'TnA_GC Cuts at: 3274 3274 Size: 5857 Bme1580I G_kGCm'C Cuts at: 1790 2188 3918 5074 1790 Size: 398 1730 1156 2573 Fragments arranged by size: 2573 1730 1156 398 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2753 3088 3588 Size: 416 53 83 399 24 1336 335 500 Cuts at: 3588 4546 442 Size: 958 1753 Fragments arranged by size: 1753 1336 958 500 416 399 335 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2814 2918 2923 3069 4517 1239 Size: 893 682 104 5 146 1448 2579 Fragments arranged by size: 2579 1448 893 682 146 104 5 Bpu10I CC'TnA_GC Cuts at: 2742 3102 3111 2742 Size: 360 9 5488 Fragments arranged by size: 5488 360 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4520 882 Size: 617 3021 2219 Fragments arranged by size: 3021 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3648 493 Size: 711 2444 2702 Fragments arranged by size: 2702 2444 711 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3664 4046 4681 275 Size: 53 83 186 936 2131 382 635 1451 Fragments arranged by size: 2131 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4290 4688 4962 5109 5520 4290 Size: 398 274 147 411 4627 Fragments arranged by size: 4627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2403 2433 2896 2926 1178 Size: 30 165 30 1000 30 463 30 4109 Fragments arranged by size: 4109 1000 463 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2759 1186 Size: 1573 4284 BseYI C'CCAG_C Cuts at: 3144 5060 5556 3144 Size: 1916 496 3445 Fragments arranged by size: 3445 1916 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5284 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3307 4068 5096 1540 Size: 275 186 1306 761 1028 2301 Fragments arranged by size: 2301 1306 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3918 4003 5074 3918 Size: 85 1071 4701 Fragments arranged by size: 4701 1071 85 BsmI GAATG_Cn' Cuts at: 2246 3368 3461 2246 Size: 1122 93 4642 Fragments arranged by size: 4642 1122 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2188 2803 3085 3176 3918 4003 5074 Size: 399 398 615 282 91 742 85 1071 Cuts at: 5074 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 615 399 398 282 91 85 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3212 844 Size: 2368 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3744 1394 Size: 429 1921 3507 Fragments arranged by size: 3507 1921 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4352 4526 65 Size: 1183 3104 174 1396 Fragments arranged by size: 3104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4501 4688 1700 Size: 110 2691 187 2869 Fragments arranged by size: 2869 2691 187 110 BsrGI T'GTAC_A Cuts at: 96 2421 96 Size: 2325 3532 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 2447 794 Size: 1653 4204 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstXI CCAn_nnnn'nTGG Cuts at: 2395 2795 2395 Size: 400 5457 BstYI r'GATC_y Cuts at: 1972 2073 2306 2867 3187 3554 3939 3956 5408 Size: 101 233 561 320 367 385 17 1452 Cuts at: 5408 5494 5506 5852 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 561 385 367 346 320 233 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4366 Fragments arranged by size: 4366 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 3373 4167 4187 3373 Size: 794 20 5043 Fragments arranged by size: 5043 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DraIII CAC_nnn'GTG Cuts at: 2914 2914 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3304 4193 5822 Size: 54 1425 50 275 18 1474 889 1629 Cuts at: 5822 8 Size: 43 Fragments arranged by size: 1629 1474 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3304 1537 Size: 275 1492 4090 Fragments arranged by size: 4090 1492 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3787 1353 Size: 2434 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4444 4818 4964 387 Size: 1064 138 2855 374 146 1280 Fragments arranged by size: 2855 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2523 2814 2874 2918 2923 3069 Size: 393 500 391 291 60 44 5 146 Cuts at: 3069 3920 4517 5304 5572 1239 Size: 851 597 787 268 1524 Fragments arranged by size: 1524 851 787 597 500 393 391 291 268 146 60 44 5 EcoO109I rG'GnC_Cy Cuts at: 2178 2178 Size: 5857 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2555 2555 Size: 5857 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 5004 1678 Size: 3326 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2053 2058 2085 2090 2311 Size: 32 135 32 481 5 27 5 221 Cuts at: 2311 2343 2420 2452 2810 2828 2842 2860 4498 Size: 32 77 32 358 18 14 18 1638 Cuts at: 4498 4530 4572 4604 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1638 481 358 221 135 77 42 32 32 32 32 32 32 27 18 18 14 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3201 3447 669 Size: 988 281 1263 246 3079 Fragments arranged by size: 3079 1263 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4971 5155 1305 Size: 3666 184 2007 Fragments arranged by size: 3666 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4035 Fragments arranged by size: 4035 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2292 2393 2793 3815 4174 4333 518 Size: 648 1126 101 400 1022 359 159 2042 Fragments arranged by size: 2042 1126 1022 648 400 359 159 101 MspA1I CmG'CkG Cuts at: 1606 2834 2874 3273 3950 5098 5343 1606 Size: 1228 40 399 677 1148 245 2120 Fragments arranged by size: 2120 1228 1148 677 399 245 40 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4366 Fragments arranged by size: 4366 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 3215 4760 1132 Size: 97 1986 1545 2229 Fragments arranged by size: 2229 1986 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5857 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 2339 1108 Size: 1231 4626 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PstI C_TGCA'G Cuts at: 830 3209 830 Size: 2379 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 2834 2874 2834 Size: 40 5817 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SexAI A'CCwGG_T Cuts at: 2332 2820 2332 Size: 488 5369 SfcI C'TryA_G Cuts at: 826 1045 3125 3205 4340 5021 5212 826 Size: 219 2080 80 1135 681 191 1471 Fragments arranged by size: 2080 1471 1135 681 219 191 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 3184 1872 Size: 1312 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 4862 5124 820 Size: 197 926 2018 901 262 1553 Fragments arranged by size: 2018 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 3027 1517 Size: 1510 4347 StyI C'CwwG_G Cuts at: 513 1065 2004 3296 513 Size: 552 939 1292 3074 Fragments arranged by size: 3074 1292 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3077 3884 3901 4054 4239 4684 325 Size: 1255 1497 807 17 153 185 445 1498 Fragments arranged by size: 1498 1497 1255 807 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 4103 5815 Size: 274 80 33 51 1443 444 1682 1712 Cuts at: 5815 96 Size: 138 Fragments arranged by size: 1712 1682 1443 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2403 2435 2896 2928 5817 5849 2403 Size: 32 461 32 2889 32 2411 Fragments arranged by size: 2889 2411 461 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2980 2980 Size: 5857 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 3182 1870 Size: 1312 4545 XmnI GAAnn'nnTTC Cuts at: 3986 3986 Size: 5857 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI