Restriction map for
pFN21AB6512
(Linear) (Six-base) MAPSORT of: KIBB6512.seq Check: 5996 from: 1 to: 1152
KIBB6512.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 60 1001 1152
Size: 60 941 151
Fragments arranged by size:
941 151 60
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 869 1152
Size: 518 351 283
Fragments arranged by size:
518 351 283
AhdI GACnn_n'nnGTC
Cuts at: 0 429 819 1152
Size: 429 390 333
Fragments arranged by size:
429 390 333
AlwNI CAG_nnn'CTG
Cuts at: 0 176 433 1152
Size: 176 257 719
Fragments arranged by size:
719 257 176
BamHI G'GATC_C
Cuts at: 0 301 862 1152
Size: 301 561 290
Fragments arranged by size:
561 301 290
BanII G_rGCy'C
Cuts at: 0 798 1080 1152
Size: 798 282 72
Fragments arranged by size:
798 282 72
BbsI GAAGACnn'nnnn_
Cuts at: 0 957 1152
Size: 957 195
BbvCI CC'TCA_GC
Cuts at: 0 737 1097 1152
Size: 737 360 55
Fragments arranged by size:
737 360 55
Bme1580I G_kGCm'C
Cuts at: 0 183 1152
Size: 183 969
BmrI ACTGGGnnnn_n'
Cuts at: 0 748 1083 1152
Size: 748 335 69
Fragments arranged by size:
748 335 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 735 1152
Size: 735 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 809 913 918 1064 1152
Size: 127 682 104 5 146 88
Fragments arranged by size:
682 146 127 104 88 5
Bpu10I CC'TnA_GC
Cuts at: 0 737 1097 1106 1152
Size: 737 360 9 46
Fragments arranged by size:
737 360 46 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 398 428 891 921 1152
Size: 398 30 463 30 231
Fragments arranged by size:
463 398 231 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 754 1152
Size: 754 398
BseYI C'CCAG_C
Cuts at: 0 1139 1152
Size: 1139 13
BsmI GAATG_Cn'
Cuts at: 0 241 1152
Size: 241 911
Bsp1286I G_dGCh'C
Cuts at: 0 183 798 1080 1152
Size: 183 615 282 72
Fragments arranged by size:
615 282 183 72
BsrGI T'GTAC_A
Cuts at: 0 416 1152
Size: 416 736
BstAPI GCAn_nnn'nTGC
Cuts at: 0 442 1152
Size: 442 710
BstXI CCAn_nnnn'nTGG
Cuts at: 0 390 790 1152
Size: 390 400 362
Fragments arranged by size:
400 390 362
BstYI r'GATC_y
Cuts at: 0 68 301 862 1152
Size: 68 233 561 290
Fragments arranged by size:
561 290 233 68
DraIII CAC_nnn'GTG
Cuts at: 0 909 1152
Size: 909 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 518 809 869 913 918 1064 1152
Size: 127 391 291 60 44 5 146 88
Fragments arranged by size:
391 291 146 127 88 60 44 5
EcoO109I rG'GnC_Cy
Cuts at: 0 173 1152
Size: 173 979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 550 1152
Size: 550 602
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 48 53 80 85 306 338 415 447
Size: 48 5 27 5 221 32 77 32
Cuts at: 447 805 823 837 855 1152
Size: 358 18 14 18 297
Fragments arranged by size:
358 297 221 77 48 32 32 27
18 18 14 5 5
MslI CAynn'nnrTG
Cuts at: 0 287 388 788 1152
Size: 287 101 400 364
Fragments arranged by size:
400 364 287 101
MspA1I CmG'CkG
Cuts at: 0 829 869 1152
Size: 829 40 283
Fragments arranged by size:
829 283 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 334 1152
Size: 334 818
PvuII CAG'CTG
Cuts at: 0 829 869 1152
Size: 829 40 283
Fragments arranged by size:
829 283 40
SexAI A'CCwGG_T
Cuts at: 0 327 815 1152
Size: 327 488 337
Fragments arranged by size:
488 337 327
SfcI C'TryA_G
Cuts at: 0 1120 1152
Size: 1120 32
StuI AGG'CCT
Cuts at: 0 1022 1152
Size: 1022 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1072 1152
Size: 1072 80
TatI w'GTAC_w
Cuts at: 0 416 1152
Size: 416 736
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 398 430 891 923 1152
Size: 398 32 461 32 229
Fragments arranged by size:
461 398 229 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 975 1152
Size: 975 177
Enzymes that do cut:
AccI AcuI AhdI AlwNI BamHI BanII BbsI BbvCI
Bme1580I BmrI BplI BpmI Bpu10I BsaXI BseRI BseYI
BsmI Bsp1286I BsrGI BstAPI BstXI BstYI DraIII Eco57MI
EcoO109I FalI Hin4I MslI MspA1I PflMI PvuII SexAI
SfcI StuI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI