################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccIII AclI AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BciVI BclI BfaI BglII BlnI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MunI NdeI NheI NotI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfuI SmaI SnaBI SpeI SspBI SspI SwaI TfiI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 2389 GACGTC GACGT^C AcsI 1566 GAATTT [GA]^AATT[CT] AflII 2269 CTTAAG C^TTAAG AlwI 2061 GATCC GGATC(4/5) AocI 835 CCTGAGG CC^T[ACGT]AGG ApoI 1566 GAATTT [GA]^AATT[CT] AspI 2386 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 122 CCCGAG C^[CT]CG[GA]G AviII 527 TGCGCA TGC^GCA BbeI 2617 GGCGCC GGCGC^C BbvCI 1560 GCTGAGG CCTCAGC(-5/-2) BfrI 2269 CTTAAG C^TTAAG BlpI 2442 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2442 GCTCAGC GC^T[ACGT]AGC BsoBI 122 CCCGAG C^[CT]CG[GA]G BsrBI 1956 CCGCTC CCGCTC(-3/-3) BssHII 2050 GCGCGC G^CGCGC BstEII 1386 GGTCACC G^GT[ACGT]ACC BstPI 1386 GGTCACC G^GT[ACGT]ACC BstYI 2060 AGATCC [GA]^GATC[CT] Bsu36I 835 CCTGAGG CC^T[ACGT]AGG CelII 2442 GCTCAGC GC^T[ACGT]AGC DrdI 382 GACTGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 835 CCTGAGG CC^T[ACGT]AGG EcoNI 2448 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1386 GGTCACC G^GT[ACGT]ACC FseI 1722 GGCCGGCC GGCCGG^CC FspI 527 TGCGCA TGC^GCA KasI 2617 GGCGCC G^GCGCC MflI 2060 AGATCC [GA]^GATC[CT] MseI 2270 TTAA T^TAA NarI 2617 GGCGCC GG^CGCC NruI 414 TCGCGA TCG^CGA PflFI 2386 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 828 GAAGAGC GCTCTTC(1/4) SbfI 792 CCTGCAGG CCTGCA^GG SfiI 1722 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2617 GGCGCC GGC^GCC SgrAI 436 CGCCGGTG C[GA]^CCGG[CT]G Sse8387I 792 CCTGCAGG CCTGCA^GG Tru9I 2270 TTAA T^TAA Tth111I 2386 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 2060 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 1525 GTCTAC GT^[AC][GT]AC 1975 GTCTAC ApaI 24 GGGCCC GGGCC^C 1370 GGGCCC BbrPI 2094 CACGTG CAC^GTG 2578 CACGTG BbsI 1234 GTCTTC GAAGAC(2/6) 2515 GTCTTC BcgI 405 GCAGGGCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2459 GCAGCCTCATCG BpuAI 1234 GTCTTC GAAGAC(2/6) 2515 GTCTTC BsaAI 2094 CACGTG [CT]AC^GT[GA] 2578 CACGTG BsaI 505 GAGACC GGTCTC(1/5) 2038 GAGACC BsmBI 1769 CGTCTC CGTCTC(1/5) 164 GAGACG EagI 2103 CGGCCG C^GGCCG 2246 CGGCCG EclXI 2103 CGGCCG C^GGCCG 2246 CGGCCG Eco52I 2103 CGGCCG C^GGCCG 2246 CGGCCG HincII 619 GTCAAC GT[CT]^[GA]AC 1183 GTCAAC HindII 619 GTCAAC GT[CT]^[GA]AC 1183 GTCAAC NcoI 1067 CCATGG C^CATGG 1892 CCATGG PmaCI 2094 CACGTG CAC^GTG 2578 CACGTG PmlI 2094 CACGTG CAC^GTG 2578 CACGTG SexAI 1484 ACCTGGT A^CC[AT]GGT 1601 ACCTGGT StuI 1106 AGGCCT AGG^CCT 2210 AGGCCT Tsp509I 1429 AATT ^AATT 1567 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI