(Linear) (Six-base) MAPSORT of: KIEE4586.seq Check: 8557 from: 1 to: 2670 KIEE4586.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 570 2670 Size: 570 2100 AatII G_ACGT'C Cuts at: 0 2393 2670 Size: 2393 277 AccI GT'mk_AC Cuts at: 0 1526 1976 2670 Size: 1526 450 694 Fragments arranged by size: 1526 694 450 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 877 1462 2670 Size: 877 585 1208 Fragments arranged by size: 1208 877 585 AflII C'TTAA_G Cuts at: 0 2269 2670 Size: 2269 401 AleI CACnn'nnGTG Cuts at: 0 376 2670 Size: 376 2294 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2024 2626 2670 Size: 2024 602 44 Fragments arranged by size: 2024 602 44 ApaI G_GGCC'C Cuts at: 0 28 1374 2670 Size: 28 1346 1296 Fragments arranged by size: 1346 1296 28 ApoI r'AATT_y Cuts at: 0 1566 2670 Size: 1566 1104 AvaI C'yCGr_G Cuts at: 0 122 2670 Size: 122 2548 BanI G'GyrC_C Cuts at: 0 226 1576 2617 2670 Size: 226 1350 1041 53 Fragments arranged by size: 1350 1041 226 53 BanII G_rGCy'C Cuts at: 0 28 835 1157 1211 1358 1374 1628 1781 Size: 28 807 322 54 147 16 254 153 Cuts at: 1781 1891 2261 2525 2670 Size: 110 370 264 145 Fragments arranged by size: 807 370 322 264 254 153 147 145 110 54 28 16 BbeI G_GCGC'C Cuts at: 0 2621 2670 Size: 2621 49 BbsI GAAGACnn'nnnn_ Cuts at: 0 1227 2508 2670 Size: 1227 1281 162 Fragments arranged by size: 1281 1227 162 BbvCI CC'TCA_GC Cuts at: 0 1561 2670 Size: 1561 1109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 394 428 2448 2482 2670 Size: 394 34 2020 34 188 Fragments arranged by size: 2020 394 188 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 925 1729 2204 2498 2670 Size: 925 804 475 294 172 Fragments arranged by size: 925 804 475 294 172 BlpI GC'TnA_GC Cuts at: 0 2443 2670 Size: 2443 227 Bme1580I G_kGCm'C Cuts at: 0 28 659 1374 1579 1598 1820 2670 Size: 28 631 715 205 19 222 850 Fragments arranged by size: 850 715 631 222 205 28 19 BmgBI CAC'GTC Cuts at: 0 467 1769 2670 Size: 467 1302 901 Fragments arranged by size: 1302 901 467 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 535 622 709 906 1225 1666 1795 Size: 295 240 87 87 197 319 441 129 Cuts at: 1795 2098 2506 2670 Size: 303 408 164 Fragments arranged by size: 441 408 319 303 295 240 197 164 129 87 87 Bpu10I CC'TnA_GC Cuts at: 0 1333 1339 1561 2670 Size: 1333 6 222 1109 Fragments arranged by size: 1333 1109 222 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1069 2509 2670 Size: 1069 1440 161 Fragments arranged by size: 1440 1069 161 BsaI GGTCTCn'nnnn_ Cuts at: 0 499 2032 2670 Size: 499 1533 638 Fragments arranged by size: 1533 638 499 BsaAI yAC'GTr Cuts at: 0 2096 2580 2670 Size: 2096 484 90 Fragments arranged by size: 2096 484 90 BsaHI Gr'CG_yC Cuts at: 0 770 2390 2618 2670 Size: 770 1620 228 52 Fragments arranged by size: 1620 770 228 52 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 904 934 2670 Size: 904 30 1736 Fragments arranged by size: 1736 904 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 123 489 2266 2269 2395 2670 Size: 123 366 1777 3 126 275 Fragments arranged by size: 1777 366 275 126 123 3 BseYI C'CCAG_C Cuts at: 0 21 194 778 2555 2670 Size: 21 173 584 1777 115 Fragments arranged by size: 1777 584 173 115 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 354 862 1062 2670 Size: 354 508 200 1608 Fragments arranged by size: 1608 508 354 200 BsiEI CG_ry'CG Cuts at: 0 2106 2249 2313 2670 Size: 2106 143 64 357 Fragments arranged by size: 2106 357 143 64 BsiHKAI G_wGCw'C Cuts at: 0 129 629 1345 1628 1781 1891 2261 2525 Size: 129 500 716 283 153 110 370 264 Cuts at: 2525 2594 2670 Size: 69 76 Fragments arranged by size: 716 500 370 283 264 153 129 110 76 69 BsmBI CGTCTCn'nnnn_ Cuts at: 0 158 1775 2670 Size: 158 1617 895 Fragments arranged by size: 1617 895 158 Bsp1286I G_dGCh'C Cuts at: 0 28 129 629 659 835 1157 1211 1345 Size: 28 101 500 30 176 322 54 134 Cuts at: 1345 1358 1374 1579 1598 1628 1781 1820 1891 Size: 13 16 205 19 30 153 39 71 Cuts at: 1891 2261 2525 2594 2670 Size: 370 264 69 76 Fragments arranged by size: 500 370 322 264 205 176 153 134 101 76 71 69 54 39 30 30 28 19 16 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 338 570 851 1145 1538 2670 Size: 338 232 281 294 393 1132 Fragments arranged by size: 1132 393 338 294 281 232 BsrBI CCG'CTC Cuts at: 0 1958 2670 Size: 1958 712 BsrFI r'CCGG_y Cuts at: 0 340 437 1306 1393 1654 1723 2101 2670 Size: 340 97 869 87 261 69 378 569 Fragments arranged by size: 869 569 378 340 261 97 87 69 BssHII G'CGCG_C Cuts at: 0 2050 2670 Size: 2050 620 BstAPI GCAn_nnn'nTGC Cuts at: 0 330 1274 1283 1583 2670 Size: 330 944 9 300 1087 Fragments arranged by size: 1087 944 330 300 9 BstEII G'GTnAC_C Cuts at: 0 1386 2670 Size: 1386 1284 BstXI CCAn_nnnn'nTGG Cuts at: 0 1659 1719 2030 2670 Size: 1659 60 311 640 Fragments arranged by size: 1659 640 311 60 BstYI r'GATC_y Cuts at: 0 2060 2670 Size: 2060 610 Bsu36I CC'TnA_GG Cuts at: 0 836 2670 Size: 836 1834 BtgI C'CryG_G Cuts at: 0 587 1067 1631 1799 1892 2243 2333 2670 Size: 587 480 564 168 93 351 90 337 Fragments arranged by size: 587 564 480 351 337 168 93 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 708 1245 2670 Size: 708 537 1425 Fragments arranged by size: 1425 708 537 BtsI GCAGTG_nn' Cuts at: 0 11 847 2670 Size: 11 836 1823 Fragments arranged by size: 1823 836 11 DrdI GACnn_nn'nnGTC Cuts at: 0 388 2670 Size: 388 2282 EaeI y'GGCC_r Cuts at: 0 338 584 612 1376 1452 1479 1721 1922 Size: 338 246 28 764 76 27 242 201 Cuts at: 1922 2103 2246 2279 2670 Size: 181 143 33 391 Fragments arranged by size: 764 391 338 246 242 201 181 143 76 33 28 27 EagI C'GGCC_G Cuts at: 0 2103 2246 2670 Size: 2103 143 424 Fragments arranged by size: 2103 424 143 EarI CTCTTCn'nnn_ Cuts at: 0 450 823 1546 1699 2670 Size: 450 373 723 153 971 Fragments arranged by size: 971 723 450 373 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 463 607 2670 Size: 463 144 2063 Fragments arranged by size: 2063 463 144 EcoICRI GAG'CTC Cuts at: 0 1626 1779 1889 2259 2523 2670 Size: 1626 153 110 370 264 147 Fragments arranged by size: 1626 370 264 153 147 110 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 535 622 709 877 906 1225 1462 Size: 295 240 87 87 168 29 319 237 Cuts at: 1462 1666 1795 2098 2506 2670 Size: 204 129 303 408 164 Fragments arranged by size: 408 319 303 295 240 237 204 168 164 129 87 87 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 2452 2670 Size: 2452 218 EcoO109I rG'GnC_Cy Cuts at: 0 25 119 294 649 840 1370 1371 1446 Size: 25 94 175 355 191 530 1 75 Cuts at: 1446 2670 Size: 1224 Fragments arranged by size: 1224 530 355 191 175 94 75 25 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1866 2649 2670 Size: 1866 783 21 Fragments arranged by size: 1866 783 21 FseI GG_CCGG'CC Cuts at: 0 1727 2670 Size: 1727 943 FspI TGC'GCA Cuts at: 0 529 2670 Size: 529 2141 HaeII r_GCGC'y Cuts at: 0 696 2147 2360 2621 2670 Size: 696 1451 213 261 49 Fragments arranged by size: 1451 696 261 213 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 904 936 2670 Size: 904 32 1734 Fragments arranged by size: 1734 904 32 HincII GTy'rAC Cuts at: 0 621 1185 2670 Size: 621 564 1485 Fragments arranged by size: 1485 621 564 KasI G'GCGC_C Cuts at: 0 2617 2670 Size: 2617 53 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 32 77 2670 Size: 32 45 2593 Fragments arranged by size: 2593 45 32 MscI TGG'CCA Cuts at: 0 1378 1454 2281 2670 Size: 1378 76 827 389 Fragments arranged by size: 1378 827 389 76 MslI CAynn'nnrTG Cuts at: 0 376 1066 1468 1717 1909 2161 2670 Size: 376 690 402 249 192 252 509 Fragments arranged by size: 690 509 402 376 252 249 192 MspA1I CmG'CkG Cuts at: 0 21 172 181 368 376 1038 1560 2354 Size: 21 151 9 187 8 662 522 794 Cuts at: 2354 2537 2670 Size: 183 133 Fragments arranged by size: 794 662 522 187 183 151 133 21 9 8 NaeI GCC'GGC Cuts at: 0 342 1395 1725 2670 Size: 342 1053 330 945 Fragments arranged by size: 1053 945 342 330 NarI GG'CG_CC Cuts at: 0 2618 2670 Size: 2618 52 NcoI C'CATG_G Cuts at: 0 1067 1892 2670 Size: 1067 825 778 Fragments arranged by size: 1067 825 778 NgoMIV G'CCGG_C Cuts at: 0 340 1393 1723 2670 Size: 340 1053 330 947 Fragments arranged by size: 1053 947 340 330 NruI TCG'CGA Cuts at: 0 416 2670 Size: 416 2254 NspI r_CATG'y Cuts at: 0 687 936 2166 2343 2670 Size: 687 249 1230 177 327 Fragments arranged by size: 1230 687 327 249 177 PfoI T'CCnGG_A Cuts at: 0 272 1094 2483 2670 Size: 272 822 1389 187 Fragments arranged by size: 1389 822 272 187 PmlI CAC'GTG Cuts at: 0 2096 2580 2670 Size: 2096 484 90 Fragments arranged by size: 2096 484 90 PpuMI rG'GwC_Cy Cuts at: 0 294 840 1446 2670 Size: 294 546 606 1224 Fragments arranged by size: 1224 606 546 294 PspOMI G'GGCC_C Cuts at: 0 24 1370 2670 Size: 24 1346 1300 Fragments arranged by size: 1346 1300 24 PstI C_TGCA'G Cuts at: 0 335 671 797 1751 2071 2227 2670 Size: 335 336 126 954 320 156 443 Fragments arranged by size: 954 443 336 335 320 156 126 PvuII CAG'CTG Cuts at: 0 368 1560 2537 2670 Size: 368 1192 977 133 Fragments arranged by size: 1192 977 368 133 SacI G_AGCT'C Cuts at: 0 1628 1781 1891 2261 2525 2670 Size: 1628 153 110 370 264 145 Fragments arranged by size: 1628 370 264 153 145 110 SanDI GG'GwC_CC Cuts at: 0 294 2670 Size: 294 2376 SapI GCTCTTCn'nnn_ Cuts at: 0 823 2670 Size: 823 1847 SbfI CC_TGCA'GG Cuts at: 0 797 2670 Size: 797 1873 SexAI A'CCwGG_T Cuts at: 0 1484 1601 2670 Size: 1484 117 1069 Fragments arranged by size: 1484 1069 117 SfcI C'TryA_G Cuts at: 0 331 667 793 1747 1809 2067 2223 2670 Size: 331 336 126 954 62 258 156 447 Fragments arranged by size: 954 447 336 331 258 156 126 62 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1729 2670 Size: 1729 941 SfoI GGC'GCC Cuts at: 0 2619 2670 Size: 2619 51 SgrAI Cr'CCGG_yG Cuts at: 0 437 2670 Size: 437 2233 SmlI C'TyrA_G Cuts at: 0 1084 2269 2524 2670 Size: 1084 1185 255 146 Fragments arranged by size: 1185 1084 255 146 SphI G_CATG'C Cuts at: 0 687 936 2343 2670 Size: 687 249 1407 327 Fragments arranged by size: 1407 687 327 249 StuI AGG'CCT Cuts at: 0 1108 2212 2670 Size: 1108 1104 458 Fragments arranged by size: 1108 1104 458 StyI C'CwwG_G Cuts at: 0 284 299 764 1067 1449 1841 1892 2396 Size: 284 15 465 303 382 392 51 504 Cuts at: 2396 2670 Size: 274 Fragments arranged by size: 504 465 392 382 303 284 274 51 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 523 1360 2071 2580 2670 Size: 523 837 711 509 90 Fragments arranged by size: 837 711 523 509 90 TatI w'GTAC_w Cuts at: 0 548 2670 Size: 548 2122 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 928 960 2670 Size: 928 32 1710 Fragments arranged by size: 1710 928 32 Tth111I GACn'n_nGTC Cuts at: 0 2389 2670 Size: 2389 281 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 93 145 1164 1854 2670 Size: 93 52 1019 690 816 Fragments arranged by size: 1019 816 690 93 52 ZraI GAC'GTC Cuts at: 0 2391 2670 Size: 2391 279 Enzymes that do cut: AarI AatII AccI AcuI AflII AleI AlfI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflIII AgeI AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII BmrI BmtI BplI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI BstZ17I ClaI CspCI DraI DraIII EcoRI EcoRV FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NsiI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SgfI SmaI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI XmnI