Restriction map for pFN21AE5220
##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BmyI        BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      Cac8I       
CfoI        Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        Tsp45I      TthHB8I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AlwI          645  GGATC                GGATC(4/5)
AspI          543  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         548  GGTCC                G^G[AT]CC
BbsI          154  GTCTTC               GAAGAC(2/6)
BfaI          361  CTAG                 C^TAG
BlpI           76  GCTAAGC              GC^T[ACGT]AGC
BpmI          200  CTCCAG               CTGGAG(16/14)
Bpu1102I       76  GCTAAGC              GC^T[ACGT]AGC
BpuAI         154  GTCTTC               GAAGAC(2/6)
BsaAI         491  CACGTA               [CT]AC^GT[GA]
BseRI         197  CTCCTC               GAGGAG(10/8)
BsgI           25  CTGCAC               GTGCAG(16/14)
BsiYI         247  CCACACACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          247  CCACACACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          32  GAGAC                GTCTC(1/5)
Bsp1407I      416  TGTACA               T^GTACA
BsrDI         593  CATTGC               GCAATG(2/0)
BsrGI         416  TGTACA               T^GTACA
BssKI         471  CCAGG                ^CC[ACGT]GG
BstF5I        232  CATCC                GGATG(2/0)
BstNI         471  CCAGG                CC^[AT]GG
BstXI         389  CCAGAATACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         645  GGATCT               [GA]^GATC[CT]
CelII          76  GCTAAGC              GC^T[ACGT]AGC
Cfr13I        548  GGTCC                G^G[ACGT]CC
DraI          672  TTTAAA               TTT^AAA
EcoRI         132  GAATTC               G^AATTC
EcoRII        471  CCAGG                ^CC[AT]GG
FokI          232  CATCC                GGATG(9/13)
HaeIII        446  GGCC                 GG^CC
MaeI          361  CTAG                 C^TAG
MaeII         492  ACGT                 A^CGT
MaeIII        477  GTTAC                ^GT[ACGT]AC
MflI          645  GGATCT               [GA]^GATC[CT]
MvaI          471  CCAGG                CC^[AT]GG
NlaIII        545  CATG                 CATG^
PflFI         543  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         247  CCACACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I        548  GGTCC                G^G[ACGT]CC
ScrFI         471  CCAGG                CC^[ACGT]GG
SmlI           46  CTCAAG               C^T[CT][GA]AG
SspBI         416  TGTACA               T^GTACA
Tth111I       543  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        247  CCACACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         645  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AccI          125  GTCTAC               GT^[AC][GT]AC
              241  GTAGAC              
AfaI          186  GTAC                 GT^AC
              417  GTAC                
AluI           12  AGCT                 AG^CT
              558  AGCT                
DpnII          43  GATC                 ^GATC
              646  GATC                
HinfI         366  GAATC                G^A[ACGT]TC
              409  GAATC               
MboI           43  GATC                 ^GATC
              646  GATC                
MslI          232  CATCCAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              491  CACGTAAATG          
NdeII          43  GATC                 ^GATC
              646  GATC                
RsaI          186  GTAC                 GT^AC
              417  GTAC                
Sau3AI         43  GATC                 ^GATC
              646  GATC                
SfaNI         320  GCATC                GCATC(5/9)
              654  GCATC               
TfiI          366  GAATC                G^A[AT]TC
              409  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AcsI        ApoI        BbvI        BsrI        DdeI        
Fnu4HI      ItaI        MboII       MnlI        MseI        
SfcI        Tru9I       TseI        Tsp509I     TspRI       
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