################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BmyI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tsp45I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AlwI 645 GGATC GGATC(4/5) AspI 543 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 548 GGTCC G^G[AT]CC BbsI 154 GTCTTC GAAGAC(2/6) BfaI 361 CTAG C^TAG BlpI 76 GCTAAGC GC^T[ACGT]AGC BpmI 200 CTCCAG CTGGAG(16/14) Bpu1102I 76 GCTAAGC GC^T[ACGT]AGC BpuAI 154 GTCTTC GAAGAC(2/6) BsaAI 491 CACGTA [CT]AC^GT[GA] BseRI 197 CTCCTC GAGGAG(10/8) BsgI 25 CTGCAC GTGCAG(16/14) BsiYI 247 CCACACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 247 CCACACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 32 GAGAC GTCTC(1/5) Bsp1407I 416 TGTACA T^GTACA BsrDI 593 CATTGC GCAATG(2/0) BsrGI 416 TGTACA T^GTACA BssKI 471 CCAGG ^CC[ACGT]GG BstF5I 232 CATCC GGATG(2/0) BstNI 471 CCAGG CC^[AT]GG BstXI 389 CCAGAATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 645 GGATCT [GA]^GATC[CT] CelII 76 GCTAAGC GC^T[ACGT]AGC Cfr13I 548 GGTCC G^G[ACGT]CC DraI 672 TTTAAA TTT^AAA EcoRI 132 GAATTC G^AATTC EcoRII 471 CCAGG ^CC[AT]GG FokI 232 CATCC GGATG(9/13) HaeIII 446 GGCC GG^CC MaeI 361 CTAG C^TAG MaeII 492 ACGT A^CGT MaeIII 477 GTTAC ^GT[ACGT]AC MflI 645 GGATCT [GA]^GATC[CT] MvaI 471 CCAGG CC^[AT]GG NlaIII 545 CATG CATG^ PflFI 543 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 247 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau96I 548 GGTCC G^G[ACGT]CC ScrFI 471 CCAGG CC^[ACGT]GG SmlI 46 CTCAAG C^T[CT][GA]AG SspBI 416 TGTACA T^GTACA Tth111I 543 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 247 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 645 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AccI 125 GTCTAC GT^[AC][GT]AC 241 GTAGAC AfaI 186 GTAC GT^AC 417 GTAC AluI 12 AGCT AG^CT 558 AGCT DpnII 43 GATC ^GATC 646 GATC HinfI 366 GAATC G^A[ACGT]TC 409 GAATC MboI 43 GATC ^GATC 646 GATC MslI 232 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 491 CACGTAAATG NdeII 43 GATC ^GATC 646 GATC RsaI 186 GTAC GT^AC 417 GTAC Sau3AI 43 GATC ^GATC 646 GATC SfaNI 320 GCATC GCATC(5/9) 654 GCATC TfiI 366 GAATC G^A[AT]TC 409 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AcsI ApoI BbvI BsrI DdeI Fnu4HI ItaI MboII MnlI MseI SfcI Tru9I TseI Tsp509I TspRI