################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboI MboII MfeI MflI MluI MroI MseI MslI MspA1I MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 164 CGCG CG^CG AcyI 83 GGCGTC G[GA]^CG[CT]C AfaI 77 GTAC GT^AC AluI 72 AGCT AG^CT Aor51HI 40 AGCGCT AGC^GCT AspHI 71 GAGCTC G[AT]GC[AT]^C BalI 35 TGGCCA TGG^CCA BanII 71 GAGCTC G[GA]GC[CT]^C BbrPI 256 CACGTG CAC^GTG BglI 9 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 71 GAGCTC G[AGT]GC[ACT]^C BsaAI 256 CACGTG [CT]AC^GT[GA] BsaHI 83 GGCGTC G[GA]^CG[CT]C BseRI 62 GAGGAG GAGGAG(10/8) BsgI 126 CTGCAC GTGCAG(16/14) BsiHKAI 71 GAGCTC G[AT]GC[AT]^C Bsp1286I 71 GAGCTC G[AGT]GC[ACT]^C BstF5I 198 CATCC GGATG(2/0) BstUI 164 CGCG CG^CG DdeI 13 CTGAG C^T[ACGT]AG DraII 16 AGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 35 TGGCCA [CT]^GGCC[GA] Eco47III 40 AGCGCT AGC^GCT EcoO109I 16 AGGCCCC [GA]G^G[ACGT]CC[CT] FauI 162 CCCGC CCCGC(4/6) FokI 198 CATCC GGATG(9/13) HapII 27 CCGG C^CGG HgaI 84 GCGTC GACGC(5/10) Hin1I 83 GGCGTC G[GA]^CG[CT]C HpaII 27 CCGG C^CGG MaeII 257 ACGT A^CGT MluNI 35 TGGCCA TGG^CCA MscI 35 TGGCCA TGG^CCA MspI 27 CCGG C^CGG MvnI 164 CGCG CG^CG NlaIV 17 GGCCCC GG[ACGT]^[ACGT]CC PmaCI 256 CACGTG CAC^GTG PmlI 256 CACGTG CAC^GTG RsaI 77 GTAC GT^AC SacI 71 GAGCTC GAGCT^C SfaNI 197 GCATC GCATC(5/9) SmaI 26 CCCGGG CCC^GGG XmaCI 26 CCCGGG C^CCGGG XmaI 26 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BbvI 43 GCTGC GCAGC(8/12) 108 GCTGC BcnI 26 CCCGG CC^[GC]GG 27 CCGGG BsmFI 224 GGGAC GGGAC(10/14) 179 GTCCC BstNI 80 CCTGG CC^[AT]GG 218 CCTGG DsaI 57 CCACGG C^C[GA][CT]GG 272 CCACGG EcoRII 80 CCTGG ^CC[AT]GG 218 CCTGG Fnu4HI 43 GCTGC GC^[ACGT]GC 108 GCTGC HaeII 40 AGCGCT [GA]GCGC^[CT] 105 GGCGCT ItaI 43 GCTGC GC^[ACGT]GC 108 GCTGC MvaI 80 CCTGG CC^[AT]GG 218 CCTGG NciI 26 CCCGG CC^[GC]GG 27 CCGGG TseI 43 GCTGC G^C[AT]GC 108 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AciI AvaI BsaJI BsiYI BslI BsoBI BssKI Cac8I CfoI Cfr13I HaeIII HhaI HinP1I MnlI MwoI Sau96I ScrFI