################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AvrII BamHI BbeI BbrPI BbsI BcgI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I CelII ClaI DraI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI HincII HindII HpaI KasI KpnI KspI MaeI MfeI MluI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI SacI SacII SalI SapI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 1524 GACGTC GACGT^C AccIII 452 TCCGGA T^CCGGA AhdI 1111 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1597 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1959 CCTCAGG CC^T[ACGT]AGG ApaI 1656 GGGCCC GGGCC^C AspEI 1111 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1678 GTGCTC G[AT]GC[AT]^C AspI 1207 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1226 TGCGCA TGC^GCA BbvCI 1191 CCTCAGC CCTCAGC(-5/-2) BclI 990 TGATCA T^GATCA BglII 1809 AGATCT A^GATCT BsaAI 1722 CACGTA [CT]AC^GT[GA] BsaBI 369 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 452 TCCGGA [AT]^CCGG[AT] BseAI 452 TCCGGA T^CCGGA BsiEI 14 CGGTCG CG[GA][CT]^CG BsiHKAI 1678 GTGCTC G[AT]GC[AT]^C BsmI 30 GAATGC GAATGC(1/-1) BspEI 452 TCCGGA T^CCGGA BspHI 1199 TCATGA T^CATGA BsrBI 1013 GAGCGG CCGCTC(-3/-3) BsrDI 1944 CATTGC GCAATG(2/0) BsrFI 1094 ACCGGC [GA]^CCGG[CT] BstBI 1768 TTCGAA TT^CGAA BstYI 1809 AGATCT [GA]^GATC[CT] Bsu36I 1959 CCTCAGG CC^T[ACGT]AGG Cfr10I 1094 ACCGGC [GA]^CCGG[CT] CpoI 625 CGGACCG CG^G[AT]CCG Eam1105I 1111 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1959 CCTCAGG CC^T[ACGT]AGG EcoRI 220 GAATTC G^AATTC FauI 1866 GCGGG CCCGC(4/6) FbaI 990 TGATCA T^GATCA FspI 1226 TGCGCA TGC^GCA HgaI 1540 GACGC GACGC(5/10) HindIII 541 AAGCTT A^AGCTT MamI 369 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1809 AGATCT [GA]^GATC[CT] MroI 452 TCCGGA T^CCGGA NspV 1768 TTCGAA TT^CGAA PflFI 1207 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 1199 TCATGA T^CATGA RsrII 625 CGGACCG CG^G[AT]CCG SbfI 1635 CCTGCAGG CCTGCA^GG SfuI 1768 TTCGAA TT^CGAA SmaI 434 CCCGGG CCC^GGG Sse8387I 1635 CCTGCAGG CCTGCA^GG StuI 1439 AGGCCT AGG^CCT Tth111I 1207 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1809 AGATCT [GA]^GATC[CT] XmaCI 434 CCCGGG C^CCGGG XmaI 434 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 220 GAATTC [GA]^AATT[CT] 296 GAATTT AcyI 1524 GACGTC G[GA]^CG[CT]C 1540 GACGCC AlwI 1247 GGATC GGATC(4/5) 1280 GGATC Aor51HI 179 AGCGCT AGC^GCT 594 AGCGCT ApoI 220 GAATTC [GA]^AATT[CT] 296 GAATTT BciVI 274 GGATAC GTATCC(6/5) 385 GGATAC BsaHI 1524 GACGTC G[GA]^CG[CT]C 1540 GACGCC BsaI 1052 GAGACC GGTCTC(1/5) 753 GGTCTC BsgI 1813 CTGCAC GTGCAG(16/14) 508 GTGCAG DsaI 1121 CCATGG C^C[GA][CT]GG 1460 CCATGG Eco47III 179 AGCGCT AGC^GCT 594 AGCGCT EcoNI 257 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1751 CCTATGTCAGG EcoT14I 1121 CCATGG C^C[AT][AT]GG 1460 CCATGG Hin1I 1524 GACGTC G[GA]^CG[CT]C 1540 GACGCC NcoI 1121 CCATGG C^CATGG 1460 CCATGG NspI 485 ACATGC [GA]CATG^[CT] 1028 GCATGT PvuII 206 CAGCTG CAG^CTG 1149 CAGCTG SmlI 776 CTCAAG C^T[CT][GA]AG 1305 CTTGAG StyI 1121 CCATGG C^C[AT][AT]GG 1460 CCATGG Tsp509I 221 AATT ^AATT 297 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AluI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I XcmI