Restriction map for
pFN21AE4495
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BseAI BseRI BsiEI
BsiWI BsmBI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstAPI BstZ17I
CelII CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NsiI NspI PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 662 GGTACC G^GTACC
AccII 441 CGCG CG^CG
AcsI 265 AAATTC [GA]^AATT[CT]
AlwNI 1255 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 502 GGGCCC GGGCC^C
ApoI 265 AAATTC [GA]^AATT[CT]
Asp718I 662 GGTACC G^GTACC
AvaI 77 CCCGAG C^[CT]CG[GA]G
BalI 256 TGGCCA TGG^CCA
BbeI 427 GGCGCC GGCGC^C
BbsI 1487 GAAGAC GAAGAC(2/6)
BclI 470 TGATCA T^GATCA
BcnI 171 CCGGG CC^[GC]GG
BfaI 103 CTAG C^TAG
BpuAI 1487 GAAGAC GAAGAC(2/6)
BsaAI 466 TACGTG [CT]AC^GT[GA]
BsaI 795 GAGACC GGTCTC(1/5)
BsgI 134 GTGCAG GTGCAG(16/14)
BsmI 1044 GAATGC GAATGC(1/-1)
BsoBI 77 CCCGAG C^[CT]CG[GA]G
BspDI 1369 ATCGAT AT^CGAT
BsrBI 355 GAGCGG CCGCTC(-3/-3)
BstBI 1493 TTCGAA TT^CGAA
BstEII 885 GGTCACC G^GT[ACGT]ACC
BstPI 885 GGTCACC G^GT[ACGT]ACC
BstUI 441 CGCG CG^CG
CfoI 428 GCGC GCG^C
ClaI 1369 ATCGAT AT^CGAT
DraIII 888 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 447 GAAGAG CTCTTC(1/4)
EcoO65I 885 GGTCACC G^GT[ACGT]ACC
FbaI 470 TGATCA T^GATCA
HaeII 427 GGCGCC [GA]GCGC^[CT]
HgaI 602 GACGC GACGC(5/10)
HhaI 428 GCGC GCG^C
HinP1I 428 GCGC G^CGC
KasI 427 GGCGCC G^GCGCC
KpnI 662 GGTACC GGTAC^C
Ksp632I 447 GAAGAG CTCTTC(1/4)
MaeI 103 CTAG C^TAG
MaeII 467 ACGT A^CGT
MluNI 256 TGGCCA TGG^CCA
MscI 256 TGGCCA TGG^CCA
MvnI 441 CGCG CG^CG
NaeI 304 GCCGGC GCC^GGC
NarI 427 GGCGCC GG^CGCC
NciI 171 CCGGG CC^[GC]GG
NgoMIV 304 GCCGGC G^CCGGC
NruI 440 TCGCGA TCG^CGA
NspV 1493 TTCGAA TT^CGAA
PpuMI 636 AGGACCT [GA]G^G[AT]CC[CT]
SexAI 881 ACCTGGT A^CC[AT]GGT
SfoI 427 GGCGCC GGC^GCC
SfuI 1493 TTCGAA TT^CGAA
XcmI 693 CCAGCGACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 427 GGCGCC G[GA]^CG[CT]C
602 GACGCC
AgeI 340 ACCGGT A^CCGGT
659 ACCGGT
Alw44I 180 GTGCAC G^TGCAC
288 GTGCAC
AlwI 805 GATCC GGATC(4/5)
1165 GATCC
AocI 403 CCTCAGG CC^T[ACGT]AGG
1252 CCTCAGG
ApaLI 180 GTGCAC G^TGCAC
288 GTGCAC
AspHI 180 GTGCAC G[AT]GC[AT]^C
288 GTGCAC
BglII 435 AGATCT A^GATCT
687 AGATCT
BsaHI 427 GGCGCC G[GA]^CG[CT]C
602 GACGCC
BsiHKAI 180 GTGCAC G[AT]GC[AT]^C
288 GTGCAC
Bsp1407I 769 TGTACA T^GTACA
1286 TGTACA
BspMI 280 ACCTGC ACCTGC(4/8)
639 ACCTGC
BsrDI 707 GCAATG GCAATG(2/0)
896 GCAATG
BsrGI 769 TGTACA T^GTACA
1286 TGTACA
BstXI 777 CCAGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1169 CCATTTTTGTGG
BstYI 435 AGATCT [GA]^GATC[CT]
687 AGATCT
Bsu36I 403 CCTCAGG CC^T[ACGT]AGG
1252 CCTCAGG
DraII 205 AGGCCCT [GA]G^G[ACGT]CC[CT]
636 AGGACCT
EaeI 143 CGGCCA [CT]^GGCC[GA]
256 TGGCCA
Eco81I 403 CCTCAGG CC^T[ACGT]AGG
1252 CCTCAGG
EcoO109I 205 AGGCCCT [GA]G^G[ACGT]CC[CT]
636 AGGACCT
Hin1I 427 GGCGCC G[GA]^CG[CT]C
602 GACGCC
HphI 473 TCACC GGTGA(8/7)
887 TCACC
MflI 435 AGATCT [GA]^GATC[CT]
687 AGATCT
MspA1I 778 CAGCTG C[AC]G^C[GT]G
840 CAGCTG
PinAI 340 ACCGGT A^CCGGT
659 ACCGGT
PstI 534 CTGCAG CTGCA^G
1238 CTGCAG
PvuII 778 CAGCTG CAG^CTG
840 CAGCTG
SfaNI 1198 GATGC GCATC(5/9)
1243 GATGC
SspBI 769 TGTACA T^GTACA
1286 TGTACA
TaqI 1370 TCGA T^CGA
1494 TCGA
TthHB8I 1370 TCGA T^CGA
1494 TCGA
XhoII 435 AGATCT [GA]^GATC[CT]
687 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BmyI BpmI BsaJI
BsaWI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI Cac8I Cfr10I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfcI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI