(Linear) (Six-base) MAPSORT of: KIEE4495.seq Check: 8753 from: 1 to: 1557 KIEE4495.orf With 182 enzymes: SgfI * March 22, 2012 18:12 .. Acc65I G'GTAC_C Cuts at: 0 662 1557 Size: 662 895 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1017 1557 Size: 1017 540 AgeI A'CCGG_T Cuts at: 0 340 659 1557 Size: 340 319 898 Fragments arranged by size: 898 340 319 AleI CACnn'nnGTG Cuts at: 0 187 1557 Size: 187 1370 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 67 205 970 1557 Size: 67 138 765 587 Fragments arranged by size: 765 587 138 67 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 376 408 1557 Size: 376 32 1149 Fragments arranged by size: 1149 376 32 AlwNI CAG_nnn'CTG Cuts at: 0 1260 1557 Size: 1260 297 ApaI G_GGCC'C Cuts at: 0 506 1557 Size: 506 1051 ApaLI G'TGCA_C Cuts at: 0 180 288 1557 Size: 180 108 1269 Fragments arranged by size: 1269 180 108 ApoI r'AATT_y Cuts at: 0 265 1557 Size: 265 1292 AvaI C'yCGr_G Cuts at: 0 77 1557 Size: 77 1480 BanI G'GyrC_C Cuts at: 0 427 656 662 1557 Size: 427 229 6 895 Fragments arranged by size: 895 427 229 6 BanII G_rGCy'C Cuts at: 0 7 63 506 1557 Size: 7 56 443 1051 Fragments arranged by size: 1051 443 56 7 BbeI G_GCGC'C Cuts at: 0 431 1557 Size: 431 1126 BbsI GAAGACnn'nnnn_ Cuts at: 0 1494 1557 Size: 1494 63 BclI T'GATC_A Cuts at: 0 470 1557 Size: 470 1087 BglII A'GATC_T Cuts at: 0 435 687 1557 Size: 435 252 870 Fragments arranged by size: 870 435 252 Bme1580I G_kGCm'C Cuts at: 0 184 292 506 1557 Size: 184 108 214 1051 Fragments arranged by size: 1051 214 184 108 BmrI ACTGGGnnnn_n' Cuts at: 0 755 1557 Size: 755 802 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 231 676 822 976 1295 1382 1557 Size: 231 445 146 154 319 87 175 Fragments arranged by size: 445 319 231 175 154 146 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 417 813 1073 1557 Size: 417 396 260 484 Fragments arranged by size: 484 417 396 260 BsaI GGTCTCn'nnnn_ Cuts at: 0 789 1557 Size: 789 768 BsaAI yAC'GTr Cuts at: 0 468 1557 Size: 468 1089 BsaHI Gr'CG_yC Cuts at: 0 428 603 1557 Size: 428 175 954 Fragments arranged by size: 954 428 175 BsaWI w'CCGG_w Cuts at: 0 275 340 659 1557 Size: 275 65 319 898 Fragments arranged by size: 898 319 275 65 BseYI C'CCAG_C Cuts at: 0 489 515 842 1557 Size: 489 26 327 715 Fragments arranged by size: 715 489 327 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 1557 Size: 155 1402 BsiHKAI G_wGCw'C Cuts at: 0 184 292 1557 Size: 184 108 1265 Fragments arranged by size: 1265 184 108 BsmI GAATG_Cn' Cuts at: 0 1050 1557 Size: 1050 507 Bsp1286I G_dGCh'C Cuts at: 0 7 63 184 292 506 1557 Size: 7 56 121 108 214 1051 Fragments arranged by size: 1051 214 121 108 56 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 289 648 1557 Size: 289 359 909 Fragments arranged by size: 909 359 289 BsrBI CCG'CTC Cuts at: 0 357 1557 Size: 357 1200 BsrDI GCAATG_nn' Cuts at: 0 714 903 1557 Size: 714 189 654 Fragments arranged by size: 714 654 189 BsrFI r'CCGG_y Cuts at: 0 304 340 659 1557 Size: 304 36 319 898 Fragments arranged by size: 898 319 304 36 BsrGI T'GTAC_A Cuts at: 0 769 1286 1557 Size: 769 517 271 Fragments arranged by size: 769 517 271 BstBI TT'CG_AA Cuts at: 0 1494 1557 Size: 1494 63 BstEII G'GTnAC_C Cuts at: 0 885 1557 Size: 885 672 BstXI CCAn_nnnn'nTGG Cuts at: 0 784 1176 1557 Size: 784 392 381 Fragments arranged by size: 784 392 381 BstYI r'GATC_y Cuts at: 0 435 687 1557 Size: 435 252 870 Fragments arranged by size: 870 435 252 Bsu36I CC'TnA_GG Cuts at: 0 404 1253 1557 Size: 404 849 304 Fragments arranged by size: 849 404 304 BtsI GCAGTG_nn' Cuts at: 0 184 543 1557 Size: 184 359 1014 Fragments arranged by size: 1014 359 184 ClaI AT'CG_AT Cuts at: 0 1370 1557 Size: 1370 187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 135 170 1557 Size: 135 35 1387 Fragments arranged by size: 1387 135 35 DraIII CAC_nnn'GTG Cuts at: 0 893 1557 Size: 893 664 EaeI y'GGCC_r Cuts at: 0 143 256 1557 Size: 143 113 1301 Fragments arranged by size: 1301 143 113 EarI CTCTTCn'nnn_ Cuts at: 0 442 1557 Size: 442 1115 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 231 676 822 976 1017 1295 1382 1557 Size: 231 445 146 154 41 278 87 175 Fragments arranged by size: 445 278 231 175 154 146 87 41 EcoO109I rG'GnC_Cy Cuts at: 0 206 637 1557 Size: 206 431 920 Fragments arranged by size: 920 431 206 HaeII r_GCGC'y Cuts at: 0 431 1557 Size: 431 1126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 912 944 970 1002 1557 Size: 912 32 26 32 555 Fragments arranged by size: 912 555 32 32 26 KasI G'GCGC_C Cuts at: 0 427 1557 Size: 427 1130 KpnI G_GTAC'C Cuts at: 0 666 1557 Size: 666 891 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 802 832 1557 Size: 802 30 725 Fragments arranged by size: 802 725 30 MscI TGG'CCA Cuts at: 0 258 1557 Size: 258 1299 MslI CAynn'nnrTG Cuts at: 0 91 187 1174 1557 Size: 91 96 987 383 Fragments arranged by size: 987 383 96 91 MspA1I CmG'CkG Cuts at: 0 780 842 1557 Size: 780 62 715 Fragments arranged by size: 780 715 62 NaeI GCC'GGC Cuts at: 0 306 1557 Size: 306 1251 NarI GG'CG_CC Cuts at: 0 428 1557 Size: 428 1129 NgoMIV G'CCGG_C Cuts at: 0 304 1557 Size: 304 1253 NruI TCG'CGA Cuts at: 0 442 1557 Size: 442 1115 PasI CC'CwG_GG Cuts at: 0 63 1557 Size: 63 1494 PpuMI rG'GwC_Cy Cuts at: 0 637 1557 Size: 637 920 PspOMI G'GGCC_C Cuts at: 0 502 1557 Size: 502 1055 PstI C_TGCA'G Cuts at: 0 538 1242 1557 Size: 538 704 315 Fragments arranged by size: 704 538 315 PvuII CAG'CTG Cuts at: 0 780 842 1557 Size: 780 62 715 Fragments arranged by size: 780 715 62 SexAI A'CCwGG_T Cuts at: 0 881 1557 Size: 881 676 SfcI C'TryA_G Cuts at: 0 534 609 1238 1356 1557 Size: 534 75 629 118 201 Fragments arranged by size: 629 534 201 118 75 SfoI GGC'GCC Cuts at: 0 429 1557 Size: 429 1128 SmlI C'TyrA_G Cuts at: 0 396 828 1088 1557 Size: 396 432 260 469 Fragments arranged by size: 469 432 396 260 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 121 1557 Size: 121 1436 TatI w'GTAC_w Cuts at: 0 453 769 1286 1557 Size: 453 316 517 271 Fragments arranged by size: 517 453 316 271 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 744 776 1424 1456 1557 Size: 744 32 648 32 101 Fragments arranged by size: 744 648 101 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 700 1557 Size: 700 857 Enzymes that do cut: Acc65I AcuI AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BclI BglII Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstXI BstYI Bsu36I BtsI ClaI CspCI DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI PasI PpuMI PspOMI PstI PvuII SexAI SfcI SfoI SmlI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AhdI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I BsaBI BsaXI BseRI BsiEI BsiWI BsmBI BspEI BspHI BssHII BssSI BstAPI BstZ17I BtgI BtgZI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NcoI NdeI NheI NotI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI