Restriction map for pFN21AE4495
 (Linear) (Six-base) MAPSORT of: KIEE4495.seq  Check: 8753  from: 1  to: 1557



KIEE4495.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:12  ..

Acc65I G'GTAC_C

Cuts at:      0     662    1557 
   Size:        662     895
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1017    1557 
   Size:       1017     540
 
AgeI A'CCGG_T

Cuts at:      0     340     659    1557 
   Size:        340     319     898

  Fragments arranged by size: 

                 898     340     319
 
AleI CACnn'nnGTG

Cuts at:      0     187    1557 
   Size:        187    1370
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      67     205     970    1557 
   Size:         67     138     765     587

  Fragments arranged by size: 

                 765     587     138      67
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     376     408    1557 
   Size:        376      32    1149

  Fragments arranged by size: 

                1149     376      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1260    1557 
   Size:       1260     297
 
ApaI G_GGCC'C

Cuts at:      0     506    1557 
   Size:        506    1051
 
ApaLI G'TGCA_C

Cuts at:      0     180     288    1557 
   Size:        180     108    1269

  Fragments arranged by size: 

                1269     180     108
 
ApoI r'AATT_y

Cuts at:      0     265    1557 
   Size:        265    1292
 
AvaI C'yCGr_G

Cuts at:      0      77    1557 
   Size:         77    1480
 
BanI G'GyrC_C

Cuts at:      0     427     656     662    1557 
   Size:        427     229       6     895

  Fragments arranged by size: 

                 895     427     229       6
 
BanII G_rGCy'C

Cuts at:      0       7      63     506    1557 
   Size:          7      56     443    1051

  Fragments arranged by size: 

                1051     443      56       7
 
BbeI G_GCGC'C

Cuts at:      0     431    1557 
   Size:        431    1126
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1494    1557 
   Size:       1494      63
 
BclI T'GATC_A

Cuts at:      0     470    1557 
   Size:        470    1087
 
BglII A'GATC_T

Cuts at:      0     435     687    1557 
   Size:        435     252     870

  Fragments arranged by size: 

                 870     435     252
 
Bme1580I G_kGCm'C

Cuts at:      0     184     292     506    1557 
   Size:        184     108     214    1051

  Fragments arranged by size: 

                1051     214     184     108
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     755    1557 
   Size:        755     802
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     676     822     976    1295    1382    1557 
   Size:        231     445     146     154     319      87     175

  Fragments arranged by size: 

                 445     319     231     175     154     146      87
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     417     813    1073    1557 
   Size:        417     396     260     484

  Fragments arranged by size: 

                 484     417     396     260
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     789    1557 
   Size:        789     768
 
BsaAI yAC'GTr

Cuts at:      0     468    1557 
   Size:        468    1089
 
BsaHI Gr'CG_yC

Cuts at:      0     428     603    1557 
   Size:        428     175     954

  Fragments arranged by size: 

                 954     428     175
 
BsaWI w'CCGG_w

Cuts at:      0     275     340     659    1557 
   Size:        275      65     319     898

  Fragments arranged by size: 

                 898     319     275      65
 
BseYI C'CCAG_C

Cuts at:      0     489     515     842    1557 
   Size:        489      26     327     715

  Fragments arranged by size: 

                 715     489     327      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155    1557 
   Size:        155    1402
 
BsiHKAI G_wGCw'C

Cuts at:      0     184     292    1557 
   Size:        184     108    1265

  Fragments arranged by size: 

                1265     184     108
 
BsmI GAATG_Cn'

Cuts at:      0    1050    1557 
   Size:       1050     507
 
Bsp1286I G_dGCh'C

Cuts at:      0       7      63     184     292     506    1557 
   Size:          7      56     121     108     214    1051

  Fragments arranged by size: 

                1051     214     121     108      56       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     289     648    1557 
   Size:        289     359     909

  Fragments arranged by size: 

                 909     359     289
 
BsrBI CCG'CTC

Cuts at:      0     357    1557 
   Size:        357    1200
 
BsrDI GCAATG_nn'

Cuts at:      0     714     903    1557 
   Size:        714     189     654

  Fragments arranged by size: 

                 714     654     189
 
BsrFI r'CCGG_y

Cuts at:      0     304     340     659    1557 
   Size:        304      36     319     898

  Fragments arranged by size: 

                 898     319     304      36
 
BsrGI T'GTAC_A

Cuts at:      0     769    1286    1557 
   Size:        769     517     271

  Fragments arranged by size: 

                 769     517     271
 
BstBI TT'CG_AA

Cuts at:      0    1494    1557 
   Size:       1494      63
 
BstEII G'GTnAC_C

Cuts at:      0     885    1557 
   Size:        885     672
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     784    1176    1557 
   Size:        784     392     381

  Fragments arranged by size: 

                 784     392     381
 
BstYI r'GATC_y

Cuts at:      0     435     687    1557 
   Size:        435     252     870

  Fragments arranged by size: 

                 870     435     252
 
Bsu36I CC'TnA_GG

Cuts at:      0     404    1253    1557 
   Size:        404     849     304

  Fragments arranged by size: 

                 849     404     304
 
BtsI GCAGTG_nn'

Cuts at:      0     184     543    1557 
   Size:        184     359    1014

  Fragments arranged by size: 

                1014     359     184
 
ClaI AT'CG_AT

Cuts at:      0    1370    1557 
   Size:       1370     187
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     135     170    1557 
   Size:        135      35    1387

  Fragments arranged by size: 

                1387     135      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     893    1557 
   Size:        893     664
 
EaeI y'GGCC_r

Cuts at:      0     143     256    1557 
   Size:        143     113    1301

  Fragments arranged by size: 

                1301     143     113
 
EarI CTCTTCn'nnn_

Cuts at:      0     442    1557 
   Size:        442    1115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     676     822     976    1017    1295    1382    1557 
   Size:        231     445     146     154      41     278      87     175

  Fragments arranged by size: 

                 445     278     231     175     154     146      87      41
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     206     637    1557 
   Size:        206     431     920

  Fragments arranged by size: 

                 920     431     206
 
HaeII r_GCGC'y

Cuts at:      0     431    1557 
   Size:        431    1126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     912     944     970    1002    1557 
   Size:        912      32      26      32     555

  Fragments arranged by size: 

                 912     555      32      32      26
 
KasI G'GCGC_C

Cuts at:      0     427    1557 
   Size:        427    1130
 
KpnI G_GTAC'C

Cuts at:      0     666    1557 
   Size:        666     891
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     802     832    1557 
   Size:        802      30     725

  Fragments arranged by size: 

                 802     725      30
 
MscI TGG'CCA

Cuts at:      0     258    1557 
   Size:        258    1299
 
MslI CAynn'nnrTG

Cuts at:      0      91     187    1174    1557 
   Size:         91      96     987     383

  Fragments arranged by size: 

                 987     383      96      91
 
MspA1I CmG'CkG

Cuts at:      0     780     842    1557 
   Size:        780      62     715

  Fragments arranged by size: 

                 780     715      62
 
NaeI GCC'GGC

Cuts at:      0     306    1557 
   Size:        306    1251
 
NarI GG'CG_CC

Cuts at:      0     428    1557 
   Size:        428    1129
 
NgoMIV G'CCGG_C

Cuts at:      0     304    1557 
   Size:        304    1253
 
NruI TCG'CGA

Cuts at:      0     442    1557 
   Size:        442    1115
 
PasI CC'CwG_GG

Cuts at:      0      63    1557 
   Size:         63    1494
 
PpuMI rG'GwC_Cy

Cuts at:      0     637    1557 
   Size:        637     920
 
PspOMI G'GGCC_C

Cuts at:      0     502    1557 
   Size:        502    1055
 
PstI C_TGCA'G

Cuts at:      0     538    1242    1557 
   Size:        538     704     315

  Fragments arranged by size: 

                 704     538     315
 
PvuII CAG'CTG

Cuts at:      0     780     842    1557 
   Size:        780      62     715

  Fragments arranged by size: 

                 780     715      62
 
SexAI A'CCwGG_T

Cuts at:      0     881    1557 
   Size:        881     676
 
SfcI C'TryA_G

Cuts at:      0     534     609    1238    1356    1557 
   Size:        534      75     629     118     201

  Fragments arranged by size: 

                 629     534     201     118      75
 
SfoI GGC'GCC

Cuts at:      0     429    1557 
   Size:        429    1128
 
SmlI C'TyrA_G

Cuts at:      0     396     828    1088    1557 
   Size:        396     432     260     469

  Fragments arranged by size: 

                 469     432     396     260
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     121    1557 
   Size:        121    1436
 
TatI w'GTAC_w

Cuts at:      0     453     769    1286    1557 
   Size:        453     316     517     271

  Fragments arranged by size: 

                 517     453     316     271
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     744     776    1424    1456    1557 
   Size:        744      32     648      32     101

  Fragments arranged by size: 

                 744     648     101      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     700    1557 
   Size:        700     857
 

 Enzymes that do cut:

   Acc65I     AcuI     AgeI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI     BclI
    BglII Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtsI     ClaI    CspCI   DraIII     EaeI     EarI  Eco57MI EcoO109I
    HaeII    Hin4I     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NruI     PasI    PpuMI   PspOMI     PstI
    PvuII    SexAI     SfcI     SfoI     SmlI    TaqII     TatI     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AhdI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BsaBI    BsaXI    BseRI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
   BssHII    BssSI   BstAPI  BstZ17I     BtgI    BtgZI     DraI     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     NcoI
     NdeI     NheI     NotI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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