################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BsrI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MluNI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 1081 TCCGGA T^CCGGA Alw44I 486 GTGCAC G^TGCAC AlwNI 43 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 82 GGGCCC GGGCC^C ApaLI 486 GTGCAC G^TGCAC AscI 24 GGCGCGCC GG^CGCGCC AspI 699 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 841 GGCGCC GGCGC^C BbsI 327 GAAGAC GAAGAC(2/6) BcgI 1319 GCAAGTGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 612 CTCCAG CTGGAG(16/14) BpuAI 327 GAAGAC GAAGAC(2/6) BseAI 1081 TCCGGA T^CCGGA BsiEI 204 CGGCCG CG[GA][CT]^CG BspEI 1081 TCCGGA T^CCGGA BspMI 964 GCAGGT ACCTGC(4/8) BsrDI 713 CATTGC GCAATG(2/0) BsrFI 29 GCCGGC [GA]^CCGG[CT] BssHII 25 GCGCGC G^CGCGC BstAPI 42 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 152 AGATCC [GA]^GATC[CT] Cfr10I 29 GCCGGC [GA]^CCGG[CT] EagI 204 CGGCCG C^GGCCG EclXI 204 CGGCCG C^GGCCG Eco52I 204 CGGCCG C^GGCCG EcoNI 1218 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1286 CCAAGG C^C[AT][AT]GG KasI 841 GGCGCC G^GCGCC MaeII 446 ACGT A^CGT MflI 152 AGATCC [GA]^GATC[CT] MroI 1081 TCCGGA T^CCGGA NaeI 29 GCCGGC GCC^GGC NarI 841 GGCGCC GG^CGCC NgoMIV 29 GCCGGC G^CCGGC NspI 665 GCATGC [GA]CATG^[CT] PflFI 699 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 765 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 217 GAGCTC GAGCT^C SapI 189 GCTCTTC GCTCTTC(1/4) SfoI 841 GGCGCC GGC^GCC SmlI 181 CTCAAG C^T[CT][GA]AG SphI 665 GCATGC GCATG^C StyI 1286 CCAAGG C^C[AT][AT]GG TfiI 289 GATTC G^A[AT]TC Tsp45I 824 GTCAC ^GT[GC]AC Tth111I 699 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 152 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 1095 GTAC GT^AC 1107 GTAC AlwI 153 GATCC GGATC(4/5) 1299 GGATC Asp700I 402 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1383 GAACGGCTTC BglI 683 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1369 GCCATCCAGGC BsaWI 398 ACCGGA [AT]^CCGG[AT] 1081 TCCGGA BsgI 316 CTGCAC GTGCAG(16/14) 895 CTGCAC BsmAI 1060 GAGAC GTCTC(1/5) 1235 GAGAC BsrBI 187 CCGCTC CCGCTC(-3/-3) 649 GAGCGG BssSI 319 CACGAG CACGAG(-5/-1) 937 CTCGTG BstXI 614 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1224 CCAGGAGTTTGG HaeII 841 GGCGCC [GA]GCGC^[CT] 1357 AGCGCC MaeIII 824 GTCAC ^GT[ACGT]AC 1199 GTAAC PleI 798 GAGTC GAGTC(4/5) 1085 GAGTC PstI 49 CTGCAG CTGCA^G 1165 CTGCAG PvuII 685 CAGCTG CAG^CTG 1420 CAGCTG RsaI 1095 GTAC GT^AC 1107 GTAC SmaI 94 CCCGGG CCC^GGG 1440 CCCGGG TaqI 194 TCGA T^CGA 1302 TCGA TthHB8I 194 TCGA T^CGA 1302 TCGA XcmI 573 CCACGCCTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1286 CCAAGGTGTTTATGG XmaCI 94 CCCGGG C^CCGGG 1440 CCCGGG XmaI 94 CCCGGG C^CCGGG 1440 CCCGGG XmnI 402 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1383 GAACGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI SacII Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI