(Circular) (Six-base) MAPSORT of: pFN21AE5613.seq Check: 693 from: 1 to: 6160 pFN21AE5613 6160 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2960 2960 Size: 6160 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3503 1937 Size: 1566 4594 Acc65I G'GTAC_C Cuts at: 3481 3481 Size: 6160 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2710 3449 4223 5607 5875 1632 Size: 1078 739 774 1384 268 1917 Fragments arranged by size: 1917 1384 1078 774 739 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 5059 6036 1203 Size: 3856 977 1327 Fragments arranged by size: 3856 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 2930 1166 Size: 1764 4396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6160 AlwNI CAG_nnn'CTG Cuts at: 2053 3903 5475 2053 Size: 1850 1572 2738 Fragments arranged by size: 2738 1850 1572 ApaI G_GGCC'C Cuts at: 2091 2091 Size: 6160 ApaLI G'TGCA_C Cuts at: 2491 4217 5373 2491 Size: 1726 1156 3278 Fragments arranged by size: 3278 1726 1156 ApoI r'AATT_y Cuts at: 1493 3469 3695 3847 5956 1493 Size: 1976 226 152 2109 1697 Fragments arranged by size: 2109 1976 1697 226 152 AscI GG'CGCG_CC Cuts at: 2030 2030 Size: 6160 AseI AT'TA_AT Cuts at: 160 1032 4714 160 Size: 872 3682 1606 Fragments arranged by size: 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2099 2788 3194 3445 3485 1870 Size: 73 147 9 689 406 251 40 4545 Fragments arranged by size: 4545 689 406 251 147 73 40 9 BamHI G'GATC_C Cuts at: 3490 3857 3490 Size: 367 5793 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2166 2846 2921 2994 3481 Size: 325 217 627 379 680 75 73 487 Cuts at: 3481 4936 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 680 627 487 379 325 217 75 73 BanII G_rGCy'C Cuts at: 1391 2091 2097 2226 3053 3479 1391 Size: 700 6 129 827 426 4072 Fragments arranged by size: 4072 827 700 426 129 6 BbeI G_GCGC'C Cuts at: 2850 2850 Size: 6160 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2339 928 Size: 971 440 4749 Fragments arranged by size: 4749 971 440 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3313 3347 4351 4385 1903 Size: 34 1376 34 1004 34 3678 Fragments arranged by size: 3678 1376 1004 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4052 5262 4052 Size: 1210 4950 BclI T'GATC_A Cuts at: 1548 1548 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2694 3380 4771 Size: 107 122 71 631 605 1022 686 1391 Cuts at: 4771 136 Size: 1525 Fragments arranged by size: 1525 1391 1022 686 631 605 122 107 71 BglII A'GATC_T Cuts at: 6155 6155 Size: 6160 BlpI GC'TnA_GC Cuts at: 3577 3577 Size: 6160 Bme1580I G_kGCm'C Cuts at: 1790 2091 2495 2924 2982 2997 3036 4221 5377 Size: 301 404 429 58 15 39 1185 1156 Cuts at: 5377 1790 Size: 2573 Fragments arranged by size: 2573 1185 1156 429 404 301 58 39 15 BmgBI CAC'GTC Cuts at: 1318 2452 1318 Size: 1134 5026 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3891 4849 442 Size: 416 53 83 399 24 2474 958 1753 Fragments arranged by size: 2474 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2602 4820 1239 Size: 1363 2218 2579 Fragments arranged by size: 2579 2218 1363 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2171 4285 5150 5448 2171 Size: 2114 865 298 2883 Fragments arranged by size: 2883 2114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4823 882 Size: 617 3324 2219 Fragments arranged by size: 3324 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3951 493 Size: 711 2747 2702 Fragments arranged by size: 2747 2702 711 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 2705 2847 3967 Size: 53 83 186 936 600 572 142 1120 Cuts at: 3967 4349 4984 275 Size: 382 635 1451 Fragments arranged by size: 1451 1120 936 635 600 572 382 186 142 83 53 BsaWI w'CCGG_w Cuts at: 2403 3086 4593 4991 5265 5412 5823 2403 Size: 683 1507 398 274 147 411 2740 Fragments arranged by size: 2740 1507 683 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5935 Fragments arranged by size: 5935 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2459 2539 2605 1186 Size: 1273 80 66 4741 Fragments arranged by size: 4741 1273 80 66 BseYI C'CCAG_C Cuts at: 2692 2917 3040 3153 5363 5859 2692 Size: 225 123 113 2210 496 2993 Fragments arranged by size: 2993 2210 496 225 123 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 2885 1163 Size: 573 570 579 4438 Fragments arranged by size: 4438 579 573 570 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2212 3610 4371 5399 1540 Size: 275 186 211 1398 761 1028 2301 Fragments arranged by size: 2301 1398 1028 761 275 211 186 BsiHKAI G_wGCw'C Cuts at: 2226 2235 2495 2502 4221 4306 5377 2226 Size: 9 260 7 1719 85 1071 3009 Fragments arranged by size: 3009 1719 1071 260 85 9 7 BsmI GAATG_Cn' Cuts at: 3671 3764 3671 Size: 93 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2097 2226 2235 2495 2502 2924 Size: 399 301 6 129 9 260 7 422 Cuts at: 2924 2982 2997 3036 3053 3479 4221 4306 5377 Size: 58 15 39 17 426 742 85 1071 Cuts at: 5377 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 426 422 399 301 260 129 85 58 39 17 15 9 7 6 BspEI T'CCGG_A Cuts at: 3086 5823 3086 Size: 2737 3423 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2960 3515 844 Size: 2116 555 3489 Fragments arranged by size: 3489 2116 555 BsrBI CCG'CTC Cuts at: 1394 1823 2194 2656 4047 1394 Size: 429 371 462 1391 3507 Fragments arranged by size: 3507 1391 462 429 371 BsrDI GCAATG_nn' Cuts at: 65 1248 2717 4655 4829 65 Size: 1183 1469 1938 174 1396 Fragments arranged by size: 1938 1469 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2034 4804 4991 1700 Size: 110 224 2770 187 2869 Fragments arranged by size: 2869 2770 224 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6160 BssHII G'CGCG_C Cuts at: 1925 2030 1925 Size: 105 6055 BssSI C'ACGA_G Cuts at: 2324 2942 4220 5232 2324 Size: 618 1278 1012 3252 Fragments arranged by size: 3252 1278 1012 618 BstAPI GCAn_nnn'nTGC Cuts at: 794 2053 794 Size: 1259 4901 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6160 BstXI CCAn_nnnn'nTGG Cuts at: 2626 3236 2626 Size: 610 5550 BstYI r'GATC_y Cuts at: 1972 2157 3490 3857 4242 4259 5711 5797 5809 Size: 185 1333 367 385 17 1452 86 12 Cuts at: 5809 6155 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1333 385 367 346 185 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2060 2073 2389 3120 513 Size: 552 939 56 13 316 731 3553 Fragments arranged by size: 3553 939 731 552 316 56 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2866 3121 487 Size: 683 56 1640 255 3526 Fragments arranged by size: 3526 1640 683 255 56 BtsI GCAGTG_nn' Cuts at: 3068 3676 4470 4490 3068 Size: 608 794 20 4738 Fragments arranged by size: 4738 794 608 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6125 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 2354 2571 Size: 54 1425 50 275 18 379 145 217 Cuts at: 2571 3060 3123 3357 3607 4496 6125 8 Size: 489 63 234 250 889 1629 43 Fragments arranged by size: 1629 1425 889 489 379 275 250 234 217 145 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2209 3607 1537 Size: 275 397 1398 4090 Fragments arranged by size: 4090 1398 397 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2150 2201 3404 4090 1353 Size: 797 51 1203 686 3423 Fragments arranged by size: 3423 1203 797 686 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2287 4747 5121 5267 387 Size: 1064 138 698 2460 374 146 1280 Fragments arranged by size: 2460 1280 1064 698 374 146 138 EcoICRI GAG'CTC Cuts at: 2224 2224 Size: 6160 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2602 2710 3449 4223 4820 5607 5875 Size: 393 970 108 739 774 597 787 268 Cuts at: 5875 1239 Size: 1524 Fragments arranged by size: 1524 970 787 774 739 597 393 268 108 EcoNI CCTnn'n_nnAGG Cuts at: 3227 3227 Size: 6160 EcoO109I rG'GnC_Cy Cuts at: 2125 2182 2507 3191 2125 Size: 57 325 684 5094 Fragments arranged by size: 5094 684 325 57 EcoRI G'AATT_C Cuts at: 3469 3847 3469 Size: 378 5782 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2212 2212 Size: 6160 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 2850 3366 5307 1678 Size: 1172 516 1941 2531 Fragments arranged by size: 2531 1941 1172 516 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3058 3090 4801 4833 4875 Size: 32 135 32 1486 32 1711 32 42 Cuts at: 4875 4907 1373 Size: 32 2626 Fragments arranged by size: 2626 1711 1486 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3504 3750 669 Size: 988 281 1566 246 3079 Fragments arranged by size: 3079 1566 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6160 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KasI G'GCGC_C Cuts at: 2846 2846 Size: 6160 KpnI G_GTAC'C Cuts at: 3485 3485 Size: 6160 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 5274 5458 1305 Size: 1116 2853 184 2007 Fragments arranged by size: 2853 2007 1116 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4338 Fragments arranged by size: 4338 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2252 2930 3107 4118 4477 4636 518 Size: 648 1086 678 177 1011 359 159 2042 Fragments arranged by size: 2042 1086 1011 678 648 359 177 159 MspA1I CmG'CkG Cuts at: 1606 2062 2085 2391 2692 2914 2930 3122 3427 Size: 456 23 306 301 222 16 192 305 Cuts at: 3427 3576 4253 5401 5646 1606 Size: 149 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 456 306 305 301 245 222 192 149 23 16 NaeI GCC'GGC Cuts at: 2036 2036 Size: 6160 NarI GG'CG_CC Cuts at: 2847 2847 Size: 6160 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4669 Fragments arranged by size: 4669 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NgoMIV G'CCGG_C Cuts at: 2034 2034 Size: 6160 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 2674 3518 5063 1132 Size: 97 1445 844 1545 2229 Fragments arranged by size: 2229 1545 1445 844 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6160 PciI A'CATG_T Cuts at: 5059 5059 Size: 6160 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6160 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4229 4261 5742 5774 4229 Size: 32 1481 32 4615 Fragments arranged by size: 4615 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2182 3191 2125 Size: 57 1009 5094 Fragments arranged by size: 5094 1009 57 PshAI GACnn'nnGTC Cuts at: 1613 2774 1613 Size: 1161 4999 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PspOMI G'GGCC_C Cuts at: 2087 2087 Size: 6160 PstI C_TGCA'G Cuts at: 830 2058 3174 3512 830 Size: 1228 1116 338 3478 Fragments arranged by size: 3478 1228 1116 338 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 PvuII CAG'CTG Cuts at: 2692 3427 2692 Size: 735 5425 SacI G_AGCT'C Cuts at: 2226 2226 Size: 6160 SacII CC_GC'GG Cuts at: 2063 2392 3123 2063 Size: 329 731 5100 Fragments arranged by size: 5100 731 329 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SapI GCTCTTCn'nnn_ Cuts at: 2201 2201 Size: 6160 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 4408 6120 4408 Size: 1712 4448 SfcI C'TryA_G Cuts at: 826 1045 2054 2734 3170 3508 4643 5324 5515 Size: 219 1009 680 436 338 1135 681 191 Cuts at: 5515 826 Size: 1471 Fragments arranged by size: 1471 1135 1009 681 680 436 338 219 191 SfoI GGC'GCC Cuts at: 2848 2848 Size: 6160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6160 SmaI CCC'GGG Cuts at: 1872 2101 3447 3487 1872 Size: 229 1346 40 4545 Fragments arranged by size: 4545 1346 229 40 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 4264 5165 5427 820 Size: 197 926 243 2078 901 262 1553 Fragments arranged by size: 2078 1553 926 901 262 243 197 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 2674 3518 1132 Size: 1542 844 3774 Fragments arranged by size: 3774 1542 844 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6160 StyI C'CwwG_G Cuts at: 513 1065 2004 3291 3599 513 Size: 552 939 1287 308 3074 Fragments arranged by size: 3074 1287 939 552 308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2893 4187 4204 4357 4542 4987 325 Size: 1255 1313 1294 17 153 185 445 1498 Fragments arranged by size: 1498 1313 1294 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3099 4406 6118 Size: 274 80 33 51 1443 1122 1307 1712 Cuts at: 6118 96 Size: 138 Fragments arranged by size: 1712 1443 1307 1122 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6120 6152 6120 Size: 32 6128 Tth111I GACn'n_nGTC Cuts at: 1316 2707 1316 Size: 1391 4769 XbaI T'CTAG_A Cuts at: 3496 3496 Size: 6160 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2585 3298 2585 Size: 713 5447 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 2099 3445 3485 1870 Size: 229 1346 40 4545 Fragments arranged by size: 4545 1346 229 40 XmnI GAAnn'nnTTC Cuts at: 2411 3392 4289 2411 Size: 981 897 4282 Fragments arranged by size: 4282 981 897 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI