(Linear) (Six-base) MAPSORT of: KIEE5613.seq Check: 9011 from: 1 to: 1455 KIEE5613.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 955 1455 Size: 955 500 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 705 1444 1455 Size: 705 739 11 Fragments arranged by size: 739 705 11 AleI CACnn'nnGTG Cuts at: 0 925 1455 Size: 925 530 AlwNI CAG_nnn'CTG Cuts at: 0 48 1455 Size: 48 1407 ApaI G_GGCC'C Cuts at: 0 86 1455 Size: 86 1369 ApaLI G'TGCA_C Cuts at: 0 486 1455 Size: 486 969 AscI GG'CGCG_CC Cuts at: 0 25 1455 Size: 25 1430 AvaI C'yCGr_G Cuts at: 0 85 94 783 1189 1440 1455 Size: 85 9 689 406 251 15 Fragments arranged by size: 689 406 251 85 15 9 BanI G'GyrC_C Cuts at: 0 161 841 916 989 1455 Size: 161 680 75 73 466 Fragments arranged by size: 680 466 161 75 73 BanII G_rGCy'C Cuts at: 0 86 92 221 1048 1455 Size: 86 6 129 827 407 Fragments arranged by size: 827 407 129 86 6 BbeI G_GCGC'C Cuts at: 0 845 1455 Size: 845 610 BbsI GAAGACnn'nnnn_ Cuts at: 0 334 1455 Size: 334 1121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1308 1342 1455 Size: 1308 34 113 Fragments arranged by size: 1308 113 34 BglI GCCn_nnn'nGGC Cuts at: 0 689 1375 1455 Size: 689 686 80 Fragments arranged by size: 689 686 80 Bme1580I G_kGCm'C Cuts at: 0 86 490 919 977 992 1031 1455 Size: 86 404 429 58 15 39 424 Fragments arranged by size: 429 424 404 86 58 39 15 BmgBI CAC'GTC Cuts at: 0 447 1455 Size: 447 1008 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 597 1455 Size: 597 858 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 1455 Size: 166 1289 BsaHI Gr'CG_yC Cuts at: 0 128 700 842 1455 Size: 128 572 142 613 Fragments arranged by size: 613 572 142 128 BsaWI w'CCGG_w Cuts at: 0 398 1081 1455 Size: 398 683 374 Fragments arranged by size: 683 398 374 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 454 534 600 1455 Size: 454 80 66 855 Fragments arranged by size: 855 454 80 66 BseYI C'CCAG_C Cuts at: 0 687 912 1035 1148 1455 Size: 687 225 123 113 307 Fragments arranged by size: 687 307 225 123 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 880 1455 Size: 301 579 575 Fragments arranged by size: 579 575 301 BsiEI CG_ry'CG Cuts at: 0 207 1455 Size: 207 1248 BsiHKAI G_wGCw'C Cuts at: 0 221 230 490 497 1455 Size: 221 9 260 7 958 Fragments arranged by size: 958 260 221 9 7 Bsp1286I G_dGCh'C Cuts at: 0 86 92 221 230 490 497 919 977 Size: 86 6 129 9 260 7 422 58 Cuts at: 977 992 1031 1048 1455 Size: 15 39 17 407 Fragments arranged by size: 422 407 260 129 86 58 39 17 15 9 7 6 BspEI T'CCGG_A Cuts at: 0 1081 1455 Size: 1081 374 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 955 1455 Size: 955 500 BsrBI CCG'CTC Cuts at: 0 189 651 1455 Size: 189 462 804 Fragments arranged by size: 804 462 189 BsrDI GCAATG_nn' Cuts at: 0 712 1455 Size: 712 743 BsrFI r'CCGG_y Cuts at: 0 29 1455 Size: 29 1426 BssHII G'CGCG_C Cuts at: 0 25 1455 Size: 25 1430 BssSI C'ACGA_G Cuts at: 0 319 937 1455 Size: 319 618 518 Fragments arranged by size: 618 518 319 BstAPI GCAn_nnn'nTGC Cuts at: 0 48 1455 Size: 48 1407 BstXI CCAn_nnnn'nTGG Cuts at: 0 621 1231 1455 Size: 621 610 224 Fragments arranged by size: 621 610 224 BstYI r'GATC_y Cuts at: 0 152 1455 Size: 152 1303 BtgI C'CryG_G Cuts at: 0 55 68 384 1115 1455 Size: 55 13 316 731 340 Fragments arranged by size: 731 340 316 55 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 861 1116 1455 Size: 861 255 339 Fragments arranged by size: 861 339 255 BtsI GCAGTG_nn' Cuts at: 0 1063 1455 Size: 1063 392 EaeI y'GGCC_r Cuts at: 0 204 349 566 1055 1118 1352 1455 Size: 204 145 217 489 63 234 103 Fragments arranged by size: 489 234 217 204 145 103 63 EagI C'GGCC_G Cuts at: 0 204 1455 Size: 204 1251 EarI CTCTTCn'nnn_ Cuts at: 0 145 196 1399 1455 Size: 145 51 1203 56 Fragments arranged by size: 1203 145 56 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 282 1455 Size: 282 1173 EcoICRI GAG'CTC Cuts at: 0 219 1455 Size: 219 1236 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 597 705 1444 1455 Size: 597 108 739 11 Fragments arranged by size: 739 597 108 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 1222 1455 Size: 1222 233 EcoO109I rG'GnC_Cy Cuts at: 0 120 177 502 1186 1455 Size: 120 57 325 684 269 Fragments arranged by size: 684 325 269 120 57 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 207 1455 Size: 207 1248 HaeII r_GCGC'y Cuts at: 0 845 1361 1455 Size: 845 516 94 Fragments arranged by size: 845 516 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1053 1085 1455 Size: 1053 32 370 Fragments arranged by size: 1053 370 32 KasI G'GCGC_C Cuts at: 0 841 1455 Size: 841 614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 416 1455 Size: 416 1039 MslI CAynn'nnrTG Cuts at: 0 247 925 1102 1455 Size: 247 678 177 353 Fragments arranged by size: 678 353 247 177 MspA1I CmG'CkG Cuts at: 0 57 80 386 687 909 925 1117 1422 Size: 57 23 306 301 222 16 192 305 Cuts at: 1422 1455 Size: 33 Fragments arranged by size: 306 305 301 222 192 57 33 23 16 NaeI GCC'GGC Cuts at: 0 31 1455 Size: 31 1424 NarI GG'CG_CC Cuts at: 0 842 1455 Size: 842 613 NgoMIV G'CCGG_C Cuts at: 0 29 1455 Size: 29 1426 NspI r_CATG'y Cuts at: 0 669 1455 Size: 669 786 PpuMI rG'GwC_Cy Cuts at: 0 120 177 1186 1455 Size: 120 57 1009 269 Fragments arranged by size: 1009 269 120 57 PshAI GACnn'nnGTC Cuts at: 0 769 1455 Size: 769 686 PspOMI G'GGCC_C Cuts at: 0 82 1455 Size: 82 1373 PstI C_TGCA'G Cuts at: 0 53 1169 1455 Size: 53 1116 286 Fragments arranged by size: 1116 286 53 PvuII CAG'CTG Cuts at: 0 687 1422 1455 Size: 687 735 33 Fragments arranged by size: 735 687 33 SacI G_AGCT'C Cuts at: 0 221 1455 Size: 221 1234 SacII CC_GC'GG Cuts at: 0 58 387 1118 1455 Size: 58 329 731 337 Fragments arranged by size: 731 337 329 58 SapI GCTCTTCn'nnn_ Cuts at: 0 196 1455 Size: 196 1259 SfcI C'TryA_G Cuts at: 0 49 729 1165 1455 Size: 49 680 436 290 Fragments arranged by size: 680 436 290 49 SfoI GGC'GCC Cuts at: 0 843 1455 Size: 843 612 SmaI CCC'GGG Cuts at: 0 96 1442 1455 Size: 96 1346 13 Fragments arranged by size: 1346 96 13 SmlI C'TyrA_G Cuts at: 0 181 1455 Size: 181 1274 SphI G_CATG'C Cuts at: 0 669 1455 Size: 669 786 StyI C'CwwG_G Cuts at: 0 1286 1455 Size: 1286 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 888 1455 Size: 888 567 TatI w'GTAC_w Cuts at: 0 1094 1455 Size: 1094 361 Tth111I GACn'n_nGTC Cuts at: 0 702 1455 Size: 702 753 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 580 1293 1455 Size: 580 713 162 Fragments arranged by size: 713 580 162 XmaI C'CCGG_G Cuts at: 0 94 1440 1455 Size: 94 1346 15 Fragments arranged by size: 1346 94 15 XmnI GAAnn'nnTTC Cuts at: 0 406 1387 1455 Size: 406 981 68 Fragments arranged by size: 981 406 68 Enzymes that do cut: AarI AcuI AleI AlwNI ApaI ApaLI AscI AvaI BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI BpmI BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI SfcI SfoI SmaI SmlI SphI StyI TaqII TatI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrGI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TstI XbaI XhoI ZraI