Restriction map for
pFN21AE1372
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
CelII ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MunI MvnI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI TaqI TthHB8I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 739 TCCGGA T^CCGGA
AcyI 782 GGCGCC G[GA]^CG[CT]C
AvaI 418 CTCGGG C^[CT]CG[GA]G
BalI 1475 TGGCCA TGG^CCA
BamHI 1497 GGATCC G^GATCC
BbeI 782 GGCGCC GGCGC^C
BbsI 1306 GAAGAC GAAGAC(2/6)
BbvCI 36 CCTCAGC CCTCAGC(-5/-2)
BpuAI 1306 GAAGAC GAAGAC(2/6)
BsaHI 782 GGCGCC G[GA]^CG[CT]C
BsaWI 739 TCCGGA [AT]^CCGG[AT]
BseAI 739 TCCGGA T^CCGGA
BsgI 937 CTGCAC GTGCAG(16/14)
BsmI 1324 GCATTC GAATGC(1/-1)
BsoBI 418 CTCGGG C^[CT]CG[GA]G
BspEI 739 TCCGGA T^CCGGA
BsrDI 278 GCAATG GCAATG(2/0)
BsrFI 317 GCCGGC [GA]^CCGG[CT]
BstXI 273 CCACAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 317 GCCGGC [GA]^CCGG[CT]
DraIII 1247 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1475 TGGCCA [CT]^GGCC[GA]
EcoNI 1473 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 782 GGCGCC G[GA]^CG[CT]C
KasI 782 GGCGCC G^GCGCC
MluNI 1475 TGGCCA TGG^CCA
MroI 739 TCCGGA T^CCGGA
MscI 1475 TGGCCA TGG^CCA
MseI 1682 TTAA T^TAA
MslI 274 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 317 GCCGGC GCC^GGC
NarI 782 GGCGCC GG^CGCC
NdeI 467 CATATG CA^TATG
NgoMIV 317 GCCGGC G^CCGGC
PshAI 699 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1119 CCTGCAGG CCTGCA^GG
SexAI 1691 ACCTGGT A^CC[AT]GGT
SfoI 782 GGCGCC GGC^GCC
SmlI 1581 CTTGAG C^T[CT][GA]AG
Sse8387I 1119 CCTGCAGG CCTGCA^GG
StuI 896 AGGCCT AGG^CCT
Tru9I 1682 TTAA T^TAA
XbaI 1503 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 109 GAATTT [GA]^AATT[CT]
1426 AAATTC
AfaI 161 GTAC GT^AC
270 GTAC
AlwNI 229 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1313 CAGCTGCTG
ApaI 1150 GGGCCC GGGCC^C
1529 GGGCCC
ApoI 109 GAATTT [GA]^AATT[CT]
1426 AAATTC
AspI 231 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1690 GACCTGGTC
BsaI 862 GAGACC GGTCTC(1/5)
1666 GGTCTC
BsmAI 862 GAGAC GTCTC(1/5)
1667 GTCTC
BspMI 163 ACCTGC ACCTGC(4/8)
935 ACCTGC
BstEII 1333 GGTTACC G^GT[ACGT]ACC
1695 GGTCACC
BstPI 1333 GGTTACC G^GT[ACGT]ACC
1695 GGTCACC
CfoI 31 GCGC GCG^C
783 GCGC
EcoO65I 1333 GGTTACC G^GT[ACGT]ACC
1695 GGTCACC
HaeII 30 GGCGCT [GA]GCGC^[CT]
782 GGCGCC
HgaI 58 GACGC GACGC(5/10)
849 GCGTC
HhaI 31 GCGC GCG^C
783 GCGC
HinP1I 31 GCGC G^CGC
783 GCGC
MaeII 535 ACGT A^CGT
704 ACGT
PflFI 231 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1690 GACCTGGTC
RsaI 161 GTAC GT^AC
270 GTAC
SapI 991 GAAGAGC GCTCTTC(1/4)
810 GCTCTTC
TfiI 426 GATTC G^A[AT]TC
1396 GAATC
Tsp509I 110 AATT ^AATT
1427 AATT
Tth111I 231 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1690 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AluI AlwI AocI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI
XcmI XhoII