(Circular) (Six-base) MAPSORT of: pFN21AE1559.seq Check: 3161 from: 1 to: 6190 pFN21AE1559 6190 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2154 3533 1937 Size: 217 1379 4594 Fragments arranged by size: 4594 1379 217 Acc65I G'GTAC_C Cuts at: 2552 3511 2552 Size: 959 5231 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2740 2901 4253 5637 5905 1632 Size: 1108 161 1352 1384 268 1917 Fragments arranged by size: 1917 1384 1352 1108 268 161 AflII C'TTAA_G Cuts at: 820 1017 2272 820 Size: 197 1255 4738 Fragments arranged by size: 4738 1255 197 AflIII A'CryG_T Cuts at: 1203 2373 3282 5089 6066 1203 Size: 1170 909 1807 977 1327 Fragments arranged by size: 1807 1327 1170 977 909 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 4919 4919 Size: 6190 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6190 AlwNI CAG_nnn'CTG Cuts at: 2254 2590 2651 2856 3043 3487 3933 5505 2254 Size: 336 61 205 187 444 446 1572 2939 Fragments arranged by size: 2939 1572 446 444 336 205 187 61 ApaLI G'TGCA_C Cuts at: 4247 5403 4247 Size: 1156 5034 ApoI r'AATT_y Cuts at: 1493 3073 3227 3499 3725 3877 5986 1493 Size: 1580 154 272 226 152 2109 1697 Fragments arranged by size: 2109 1697 1580 272 226 154 152 AseI AT'TA_AT Cuts at: 160 1032 4744 160 Size: 872 3712 1606 Fragments arranged by size: 3712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 3015 3515 1870 Size: 73 1072 500 4545 Fragments arranged by size: 4545 1072 500 73 BamHI G'GATC_C Cuts at: 3109 3520 3887 3109 Size: 411 367 5412 Fragments arranged by size: 5412 411 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 2552 2864 3511 4966 Size: 325 217 627 479 286 312 647 1455 Cuts at: 4966 618 Size: 1842 Fragments arranged by size: 1842 1455 647 627 479 325 312 286 217 BanII G_rGCy'C Cuts at: 1391 2207 2315 2342 2463 2668 2890 2930 3024 Size: 816 108 27 121 205 222 40 94 Cuts at: 3024 3481 3509 1391 Size: 457 28 4072 Fragments arranged by size: 4072 816 457 222 205 121 108 94 40 28 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2420 928 Size: 971 521 4698 Fragments arranged by size: 4698 971 521 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4381 4415 1903 Size: 34 50 34 2360 34 3678 Fragments arranged by size: 3678 2360 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4082 5292 4082 Size: 1210 4980 BclI T'GATC_A Cuts at: 1548 1548 Size: 6190 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4801 136 Size: 107 122 71 631 605 3129 1525 Fragments arranged by size: 3129 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6185 6185 Size: 6190 BlpI GC'TnA_GC Cuts at: 2081 3607 2081 Size: 1526 4664 Bme1580I G_kGCm'C Cuts at: 1790 2271 4251 5407 1790 Size: 481 1980 1156 2573 Fragments arranged by size: 2573 1980 1156 481 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2220 2345 3043 Size: 416 53 83 399 24 803 125 698 Cuts at: 3043 3921 4879 442 Size: 878 958 1753 Fragments arranged by size: 1753 958 878 803 698 416 399 125 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2439 2600 2612 2765 4850 1239 Size: 929 271 161 12 153 2085 2579 Fragments arranged by size: 2579 2085 929 271 161 153 12 Bpu10I CC'TnA_GC Cuts at: 2806 2806 Size: 6190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2144 2713 4315 5180 5478 2144 Size: 569 1602 865 298 2856 Fragments arranged by size: 2856 1602 865 569 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2022 4853 882 Size: 617 523 2831 2219 Fragments arranged by size: 2831 2219 617 523 BsaAI yAC'GTr Cuts at: 493 1204 3349 3981 493 Size: 711 2145 632 2702 Fragments arranged by size: 2702 2145 711 632 BsaBI GATnn'nnATC Cuts at: 6184 6184 Size: 6190 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3997 4379 5014 275 Size: 53 83 186 936 2464 382 635 1451 Fragments arranged by size: 2464 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4623 5021 5295 5442 5853 4623 Size: 398 274 147 411 4960 Fragments arranged by size: 4960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2407 2437 2455 2467 2485 Size: 30 165 30 1004 30 18 12 18 Cuts at: 2485 2933 2948 2963 2978 3434 3464 1178 Size: 448 15 15 15 456 30 3904 Fragments arranged by size: 3904 1004 456 448 165 30 30 30 30 18 18 15 15 15 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2460 2957 3418 3439 1186 Size: 1274 497 461 21 3937 Fragments arranged by size: 3937 1274 497 461 21 BseYI C'CCAG_C Cuts at: 2047 2193 5393 5889 2047 Size: 146 3200 496 2348 Fragments arranged by size: 3200 2348 496 146 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2846 1163 Size: 573 1110 4507 Fragments arranged by size: 4507 1110 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3640 4401 5429 1540 Size: 275 186 1639 761 1028 2301 Fragments arranged by size: 2301 1639 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2207 2315 2930 3171 4251 4336 5407 2207 Size: 108 615 241 1080 85 1071 2990 Fragments arranged by size: 2990 1080 1071 615 241 108 85 BsmI GAATG_Cn' Cuts at: 3701 3794 3701 Size: 93 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2207 2271 2315 2342 2463 2668 2890 Size: 399 417 64 44 27 121 205 222 Cuts at: 2890 2930 3024 3171 3481 3509 4251 4336 5407 Size: 40 94 147 310 28 742 85 1071 Cuts at: 5407 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 417 399 310 222 205 147 121 94 85 64 44 40 28 27 BspEI T'CCGG_A Cuts at: 5853 5853 Size: 6190 BspHI T'CATG_A Cuts at: 4079 5809 4079 Size: 1730 4460 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 2405 3545 844 Size: 1208 353 1140 3489 Fragments arranged by size: 3489 1208 1140 353 BsrBI CCG'CTC Cuts at: 1394 1823 4077 1394 Size: 429 2254 3507 Fragments arranged by size: 3507 2254 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2749 4685 4859 65 Size: 1183 1501 1936 174 1396 Fragments arranged by size: 1936 1501 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2516 4834 5021 1700 Size: 110 706 2318 187 2869 Fragments arranged by size: 2869 2318 706 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6190 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6190 BssSI C'ACGA_G Cuts at: 4250 5262 4250 Size: 1012 5178 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6190 BstBI TT'CG_AA Cuts at: 4994 4994 Size: 6190 BstEII G'GTnAC_C Cuts at: 1184 3002 1184 Size: 1818 4372 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2712 2780 3257 2011 Size: 701 68 477 4944 Fragments arranged by size: 4944 701 477 68 BstYI r'GATC_y Cuts at: 1972 2718 3109 3520 3887 4272 4289 5741 5827 Size: 746 391 411 367 385 17 1452 86 Cuts at: 5827 5839 6185 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 746 411 391 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2010 2684 2868 513 Size: 552 945 674 184 3835 Fragments arranged by size: 3835 945 674 552 184 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3278 3407 487 Size: 683 56 2052 129 3270 Fragments arranged by size: 3270 2052 683 129 56 BtsI GCAGTG_nn' Cuts at: 2219 2969 3706 4500 4520 2219 Size: 750 737 794 20 3889 Fragments arranged by size: 3889 794 750 737 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3044 3079 529 Size: 35 2480 35 3640 Fragments arranged by size: 3640 2480 35 35 DraI TTT'AAA Cuts at: 3494 3494 Size: 6190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2503 2782 Size: 54 1425 50 275 18 201 472 279 Cuts at: 2782 3129 3637 4526 6155 8 Size: 347 508 889 1629 43 Fragments arranged by size: 1629 1425 889 508 472 347 279 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3637 1537 Size: 275 1825 4090 Fragments arranged by size: 4090 1825 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2920 3029 4120 1353 Size: 1567 109 1091 3423 Fragments arranged by size: 3423 1567 1091 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3221 4777 5151 5297 387 Size: 1064 138 1632 1556 374 146 1280 Fragments arranged by size: 1632 1556 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2205 2313 2928 2205 Size: 108 615 5467 Fragments arranged by size: 5467 615 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2439 2600 2612 2740 2765 2901 Size: 393 536 271 161 12 128 25 136 Cuts at: 2901 4253 4850 5637 5905 1239 Size: 1352 597 787 268 1524 Fragments arranged by size: 1524 1352 787 597 536 393 271 268 161 136 128 25 12 EcoO109I rG'GnC_Cy Cuts at: 2221 2436 2587 2221 Size: 215 151 5824 Fragments arranged by size: 5824 215 151 EcoRI G'AATT_C Cuts at: 3499 3877 3499 Size: 378 5812 FspI TGC'GCA Cuts at: 4696 4696 Size: 6190 HaeII r_GCGC'y Cuts at: 1678 3250 5337 1678 Size: 1572 2087 2531 Fragments arranged by size: 2531 2087 1572 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2407 2439 2996 3028 3434 Size: 32 135 32 835 32 557 32 406 Cuts at: 3434 3466 4831 4863 4905 4937 1373 Size: 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1365 835 557 406 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2977 3534 3780 669 Size: 988 281 1039 557 246 3079 Fragments arranged by size: 3079 1039 988 557 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6190 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KpnI G_GTAC'C Cuts at: 2556 3515 2556 Size: 959 5231 MfeI C'AATT_G Cuts at: 3789 3789 Size: 6190 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2148 2373 2450 3477 5304 5488 1305 Size: 843 225 77 1027 1827 184 2007 Fragments arranged by size: 2007 1827 1027 843 225 184 77 MscI TGG'CCA Cuts at: 10 64 1489 1832 2033 10 Size: 54 1425 343 201 4167 Fragments arranged by size: 4167 1425 343 201 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4148 4507 4666 518 Size: 648 843 2139 359 159 2042 Fragments arranged by size: 2139 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2085 2146 2251 2485 2538 3606 4283 Size: 423 56 61 105 234 53 1068 677 Cuts at: 4283 5431 5676 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1068 677 423 245 234 105 61 56 53 NaeI GCC'GGC Cuts at: 2518 2518 Size: 6190 NcoI C'CATG_G Cuts at: 513 1065 2010 513 Size: 552 945 4693 Fragments arranged by size: 4693 945 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6190 NgoMIV G'CCGG_C Cuts at: 2516 2516 Size: 6190 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 3637 3637 Size: 6190 NspI r_CATG'y Cuts at: 1132 1229 2377 3105 3286 3548 5093 1132 Size: 97 1148 728 181 262 1545 2229 Fragments arranged by size: 2229 1545 1148 728 262 181 97 PasI CC'CwG_GG Cuts at: 1348 2675 1348 Size: 1327 4863 PciI A'CATG_T Cuts at: 2373 3282 5089 2373 Size: 909 1807 3474 Fragments arranged by size: 3474 1807 909 PflMI CCAn_nnn'nTGG Cuts at: 1108 2214 2590 1108 Size: 1106 376 4708 Fragments arranged by size: 4708 1106 376 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PmlI CAC'GTG Cuts at: 3349 3349 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2221 2587 2221 Size: 366 5824 PshAI GACnn'nnGTC Cuts at: 1613 2226 2376 1613 Size: 613 150 5427 Fragments arranged by size: 5427 613 150 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PstI C_TGCA'G Cuts at: 830 2357 2484 3542 830 Size: 1527 127 1058 3478 Fragments arranged by size: 3478 1527 1058 127 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 PvuII CAG'CTG Cuts at: 2085 2146 2485 2085 Size: 61 339 5790 Fragments arranged by size: 5790 339 61 SacI G_AGCT'C Cuts at: 2207 2315 2930 2207 Size: 108 615 5467 Fragments arranged by size: 5467 615 108 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SapI GCTCTTCn'nnn_ Cuts at: 2920 3029 2920 Size: 109 6081 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SfcI C'TryA_G Cuts at: 826 1045 2353 2480 2533 3538 4673 5354 5545 Size: 219 1308 127 53 1005 1135 681 191 Cuts at: 5545 826 Size: 1471 Fragments arranged by size: 1471 1308 1135 1005 681 219 191 127 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 3017 3517 1872 Size: 1145 500 4545 Fragments arranged by size: 4545 1145 500 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 2272 2728 4294 5195 5457 Size: 197 926 216 113 456 1566 901 262 Cuts at: 5457 820 Size: 1553 Fragments arranged by size: 1566 1553 926 901 456 262 216 197 113 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 3548 1132 Size: 2416 3774 SspI AAT'ATT Cuts at: 5 52 4114 5 Size: 47 4062 2081 Fragments arranged by size: 4062 2081 47 StuI AGG'CCT Cuts at: 1517 3126 1517 Size: 1609 4581 StyI C'CwwG_G Cuts at: 513 1065 2010 2278 2820 3629 513 Size: 552 945 268 542 809 3074 Fragments arranged by size: 3074 945 809 552 542 268 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4217 4234 4387 4572 5017 325 Size: 1255 2637 17 153 185 445 1498 Fragments arranged by size: 2637 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4436 6148 96 Size: 274 80 33 51 1443 2459 1712 138 Fragments arranged by size: 2459 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2437 2455 2469 2487 2933 2948 2965 2980 6150 Size: 18 14 18 446 15 17 15 3170 Cuts at: 6150 6182 2437 Size: 32 2445 Fragments arranged by size: 3170 2445 446 32 18 18 17 15 15 14 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2482 2482 Size: 6190 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6190 XmaI C'CCGG_G Cuts at: 1870 3015 3515 1870 Size: 1145 500 4545 Fragments arranged by size: 4545 1145 500 XmnI GAAnn'nnTTC Cuts at: 3227 3414 4319 3227 Size: 187 905 5098 Fragments arranged by size: 5098 905 187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI