Restriction map for pFN21AE1559
 (Linear) (Six-base) MAPSORT of: KIEE1559.seq  Check: 9393  from: 1  to: 1485



KIEE1559.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

AccI GT'mk_AC

Cuts at:      0     149    1485 
   Size:        149    1336
 
Acc65I G'GTAC_C

Cuts at:      0     547    1485 
   Size:        547     938
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     735     896    1485 
   Size:        735     161     589

  Fragments arranged by size: 

                 735     589     161
 
AflII C'TTAA_G

Cuts at:      0     267    1485 
   Size:        267    1218
 
AflIII A'CryG_T

Cuts at:      0     368    1277    1485 
   Size:        368     909     208

  Fragments arranged by size: 

                 909     368     208
 
AlwNI CAG_nnn'CTG

Cuts at:      0     249     585     646     851    1038    1482    1485 
   Size:        249     336      61     205     187     444       3

  Fragments arranged by size: 

                 444     336     249     205     187      61       3
 
ApoI r'AATT_y

Cuts at:      0    1068    1222    1485 
   Size:       1068     154     263

  Fragments arranged by size: 

                1068     263     154
 
AvaI C'yCGr_G

Cuts at:      0    1010    1485 
   Size:       1010     475
 
BamHI G'GATC_C

Cuts at:      0    1104    1485 
   Size:       1104     381
 
BanI G'GyrC_C

Cuts at:      0     261     547     859    1485 
   Size:        261     286     312     626

  Fragments arranged by size: 

                 626     312     286     261
 
BanII G_rGCy'C

Cuts at:      0     202     310     337     458     663     885     925    1019 
   Size:        202     108      27     121     205     222      40      94

Cuts at:   1019    1476    1485 
   Size:        457       9

  Fragments arranged by size: 

                 457     222     205     202     121     108      94      40
                  27       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     415    1485 
   Size:        415    1070
 
BlpI GC'TnA_GC

Cuts at:      0      76    1485 
   Size:         76    1409
 
Bme1580I G_kGCm'C

Cuts at:      0     266    1485 
   Size:        266    1219
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     215     340    1038    1485 
   Size:        215     125     698     447

  Fragments arranged by size: 

                 698     447     215     125
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     434     595     607     760    1485 
   Size:        163     271     161      12     153     725

  Fragments arranged by size: 

                 725     271     163     161     153      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     801    1485 
   Size:        801     684
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     708    1485 
   Size:        139     569     777

  Fragments arranged by size: 

                 777     569     139
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      17    1485 
   Size:         17    1468
 
BsaAI yAC'GTr

Cuts at:      0    1344    1485 
   Size:       1344     141
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     402     432     450     462     480     928     943     958 
   Size:        402      30      18      12      18     448      15      15

Cuts at:    958     973    1429    1459    1485 
   Size:         15     456      30      26

  Fragments arranged by size: 

                 456     448     402      30      30      26      18      18
                  15      15      15      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     455     952    1413    1434    1485 
   Size:        455     497     461      21      51

  Fragments arranged by size: 

                 497     461     455      51      21
 
BseYI C'CCAG_C

Cuts at:      0      42     188    1485 
   Size:         42     146    1297

  Fragments arranged by size: 

                1297     146      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     841    1485 
   Size:        841     644
 
BsiHKAI G_wGCw'C

Cuts at:      0     202     310     925    1166    1485 
   Size:        202     108     615     241     319

  Fragments arranged by size: 

                 615     319     241     202     108
 
Bsp1286I G_dGCh'C

Cuts at:      0     202     266     310     337     458     663     885     925 
   Size:        202      64      44      27     121     205     222      40

Cuts at:    925    1019    1166    1476    1485 
   Size:         94     147     310       9

  Fragments arranged by size: 

                 310     222     205     202     147     121      94      64
                  44      40      27       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      47     400    1485 
   Size:         47     353    1085

  Fragments arranged by size: 

                1085     353      47
 
BsrDI GCAATG_nn'

Cuts at:      0     744    1485 
   Size:        744     741
 
BsrFI r'CCGG_y

Cuts at:      0     511    1485 
   Size:        511     974
 
BstEII G'GTnAC_C

Cuts at:      0     997    1485 
   Size:        997     488
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     707     775    1252    1485 
   Size:        707      68     477     233

  Fragments arranged by size: 

                 707     477     233      68
 
BstYI r'GATC_y

Cuts at:      0     713    1104    1485 
   Size:        713     391     381

  Fragments arranged by size: 

                 713     391     381
 
BtgI C'CryG_G

Cuts at:      0       5     679     863    1485 
   Size:          5     674     184     622

  Fragments arranged by size: 

                 674     622     184       5
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1273    1402    1485 
   Size:       1273     129      83

  Fragments arranged by size: 

                1273     129      83
 
BtsI GCAGTG_nn'

Cuts at:      0     214     964    1485 
   Size:        214     750     521

  Fragments arranged by size: 

                 750     521     214
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1039    1074    1485 
   Size:       1039      35     411

  Fragments arranged by size: 

                1039     411      35
 
EaeI y'GGCC_r

Cuts at:      0      26     498     777    1124    1485 
   Size:         26     472     279     347     361

  Fragments arranged by size: 

                 472     361     347     279      26
 
EarI CTCTTCn'nnn_

Cuts at:      0     915    1024    1485 
   Size:        915     109     461

  Fragments arranged by size: 

                 915     461     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1216    1485 
   Size:       1216     269
 
EcoICRI GAG'CTC

Cuts at:      0     200     308     923    1485 
   Size:        200     108     615     562

  Fragments arranged by size: 

                 615     562     200     108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     434     595     607     735     760     896    1485 
   Size:        163     271     161      12     128      25     136     589

  Fragments arranged by size: 

                 589     271     163     161     136     128      25      12
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     216     431     582    1485 
   Size:        216     215     151     903

  Fragments arranged by size: 

                 903     216     215     151
 
HaeII r_GCGC'y

Cuts at:      0    1245    1485 
   Size:       1245     240
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     402     434     991    1023    1429    1461    1485 
   Size:        402      32     557      32     406      32      24

  Fragments arranged by size: 

                 557     406     402      32      32      32      24
 
HincII GTy'rAC

Cuts at:      0     972    1485 
   Size:        972     513
 
KpnI G_GTAC'C

Cuts at:      0     551    1485 
   Size:        551     934
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     368     445    1472    1485 
   Size:        143     225      77    1027      13

  Fragments arranged by size: 

                1027     225     143      77      13
 
MscI TGG'CCA

Cuts at:      0      28    1485 
   Size:         28    1457
 
MspA1I CmG'CkG

Cuts at:      0      24      80     141     246     480     533    1485 
   Size:         24      56      61     105     234      53     952

  Fragments arranged by size: 

                 952     234     105      61      56      53      24
 
NaeI GCC'GGC

Cuts at:      0     513    1485 
   Size:        513     972
 
NcoI C'CATG_G

Cuts at:      0       5    1485 
   Size:          5    1480
 
NgoMIV G'CCGG_C

Cuts at:      0     511    1485 
   Size:        511     974
 
NspI r_CATG'y

Cuts at:      0     372    1100    1281    1485 
   Size:        372     728     181     204

  Fragments arranged by size: 

                 728     372     204     181
 
PasI CC'CwG_GG

Cuts at:      0     670    1485 
   Size:        670     815
 
PciI A'CATG_T

Cuts at:      0     368    1277    1485 
   Size:        368     909     208

  Fragments arranged by size: 

                 909     368     208
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     209     585    1485 
   Size:        209     376     900

  Fragments arranged by size: 

                 900     376     209
 
PmlI CAC'GTG

Cuts at:      0    1344    1485 
   Size:       1344     141
 
PpuMI rG'GwC_Cy

Cuts at:      0     216     582    1485 
   Size:        216     366     903

  Fragments arranged by size: 

                 903     366     216
 
PshAI GACnn'nnGTC

Cuts at:      0     221     371    1485 
   Size:        221     150    1114

  Fragments arranged by size: 

                1114     221     150
 
PstI C_TGCA'G

Cuts at:      0     352     479    1485 
   Size:        352     127    1006

  Fragments arranged by size: 

                1006     352     127
 
PvuII CAG'CTG

Cuts at:      0      80     141     480    1485 
   Size:         80      61     339    1005

  Fragments arranged by size: 

                1005     339      80      61
 
SacI G_AGCT'C

Cuts at:      0     202     310     925    1485 
   Size:        202     108     615     560

  Fragments arranged by size: 

                 615     560     202     108
 
SapI GCTCTTCn'nnn_

Cuts at:      0     915    1024    1485 
   Size:        915     109     461

  Fragments arranged by size: 

                 915     461     109
 
SfcI C'TryA_G

Cuts at:      0     348     475     528    1485 
   Size:        348     127      53     957

  Fragments arranged by size: 

                 957     348     127      53
 
SmaI CCC'GGG

Cuts at:      0    1012    1485 
   Size:       1012     473
 
SmlI C'TyrA_G

Cuts at:      0     154     267     723    1485 
   Size:        154     113     456     762

  Fragments arranged by size: 

                 762     456     154     113
 
StuI AGG'CCT

Cuts at:      0    1121    1485 
   Size:       1121     364
 
StyI C'CwwG_G

Cuts at:      0       5     273     815    1485 
   Size:          5     268     542     670

  Fragments arranged by size: 

                 670     542     268       5
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     432     450     464     482     928     943     960     975 
   Size:        432      18      14      18     446      15      17      15

Cuts at:    975    1485 
   Size:        510

  Fragments arranged by size: 

                 510     446     432      18      18      17      15      15
                  14
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     477    1485 
   Size:        477    1008
 
XmaI C'CCGG_G

Cuts at:      0    1010    1485 
   Size:       1010     475
 
XmnI GAAnn'nnTTC

Cuts at:      0    1222    1409    1485 
   Size:       1222     187      76

  Fragments arranged by size: 

                1222     187      76
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII   AflIII    AlwNI     ApoI     AvaI
    BamHI     BanI    BanII     BbsI     BlpI Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI    CspCI     EaeI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    HaeII    Hin4I   HincII     KpnI     MmeI     MscI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI
     PmlI    PpuMI    PshAI     PstI    PvuII     SacI     SapI     SfcI
     SmaI     SmlI     StuI     StyI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmtI     BplI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI
   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI     DraI   DraIII     DrdI
     EagI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     MfeI     MluI     MslI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PfoI     PmeI     PpiI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
  Tth111I     XbaI     XhoI     ZraI
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