(Linear) (Six-base) MAPSORT of: KIEE1559.seq Check: 9393 from: 1 to: 1485 KIEE1559.orf With 182 enzymes: SgfI * July 15, 2010 18:42 .. AccI GT'mk_AC Cuts at: 0 149 1485 Size: 149 1336 Acc65I G'GTAC_C Cuts at: 0 547 1485 Size: 547 938 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 735 896 1485 Size: 735 161 589 Fragments arranged by size: 735 589 161 AflII C'TTAA_G Cuts at: 0 267 1485 Size: 267 1218 AflIII A'CryG_T Cuts at: 0 368 1277 1485 Size: 368 909 208 Fragments arranged by size: 909 368 208 AlwNI CAG_nnn'CTG Cuts at: 0 249 585 646 851 1038 1482 1485 Size: 249 336 61 205 187 444 3 Fragments arranged by size: 444 336 249 205 187 61 3 ApoI r'AATT_y Cuts at: 0 1068 1222 1485 Size: 1068 154 263 Fragments arranged by size: 1068 263 154 AvaI C'yCGr_G Cuts at: 0 1010 1485 Size: 1010 475 BamHI G'GATC_C Cuts at: 0 1104 1485 Size: 1104 381 BanI G'GyrC_C Cuts at: 0 261 547 859 1485 Size: 261 286 312 626 Fragments arranged by size: 626 312 286 261 BanII G_rGCy'C Cuts at: 0 202 310 337 458 663 885 925 1019 Size: 202 108 27 121 205 222 40 94 Cuts at: 1019 1476 1485 Size: 457 9 Fragments arranged by size: 457 222 205 202 121 108 94 40 27 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 415 1485 Size: 415 1070 BlpI GC'TnA_GC Cuts at: 0 76 1485 Size: 76 1409 Bme1580I G_kGCm'C Cuts at: 0 266 1485 Size: 266 1219 BmrI ACTGGGnnnn_n' Cuts at: 0 215 340 1038 1485 Size: 215 125 698 447 Fragments arranged by size: 698 447 215 125 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 434 595 607 760 1485 Size: 163 271 161 12 153 725 Fragments arranged by size: 725 271 163 161 153 12 Bpu10I CC'TnA_GC Cuts at: 0 801 1485 Size: 801 684 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 708 1485 Size: 139 569 777 Fragments arranged by size: 777 569 139 BsaI GGTCTCn'nnnn_ Cuts at: 0 17 1485 Size: 17 1468 BsaAI yAC'GTr Cuts at: 0 1344 1485 Size: 1344 141 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 402 432 450 462 480 928 943 958 Size: 402 30 18 12 18 448 15 15 Cuts at: 958 973 1429 1459 1485 Size: 15 456 30 26 Fragments arranged by size: 456 448 402 30 30 26 18 18 15 15 15 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 455 952 1413 1434 1485 Size: 455 497 461 21 51 Fragments arranged by size: 497 461 455 51 21 BseYI C'CCAG_C Cuts at: 0 42 188 1485 Size: 42 146 1297 Fragments arranged by size: 1297 146 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 841 1485 Size: 841 644 BsiHKAI G_wGCw'C Cuts at: 0 202 310 925 1166 1485 Size: 202 108 615 241 319 Fragments arranged by size: 615 319 241 202 108 Bsp1286I G_dGCh'C Cuts at: 0 202 266 310 337 458 663 885 925 Size: 202 64 44 27 121 205 222 40 Cuts at: 925 1019 1166 1476 1485 Size: 94 147 310 9 Fragments arranged by size: 310 222 205 202 147 121 94 64 44 40 27 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 47 400 1485 Size: 47 353 1085 Fragments arranged by size: 1085 353 47 BsrDI GCAATG_nn' Cuts at: 0 744 1485 Size: 744 741 BsrFI r'CCGG_y Cuts at: 0 511 1485 Size: 511 974 BstEII G'GTnAC_C Cuts at: 0 997 1485 Size: 997 488 BstXI CCAn_nnnn'nTGG Cuts at: 0 707 775 1252 1485 Size: 707 68 477 233 Fragments arranged by size: 707 477 233 68 BstYI r'GATC_y Cuts at: 0 713 1104 1485 Size: 713 391 381 Fragments arranged by size: 713 391 381 BtgI C'CryG_G Cuts at: 0 5 679 863 1485 Size: 5 674 184 622 Fragments arranged by size: 674 622 184 5 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1273 1402 1485 Size: 1273 129 83 Fragments arranged by size: 1273 129 83 BtsI GCAGTG_nn' Cuts at: 0 214 964 1485 Size: 214 750 521 Fragments arranged by size: 750 521 214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1039 1074 1485 Size: 1039 35 411 Fragments arranged by size: 1039 411 35 EaeI y'GGCC_r Cuts at: 0 26 498 777 1124 1485 Size: 26 472 279 347 361 Fragments arranged by size: 472 361 347 279 26 EarI CTCTTCn'nnn_ Cuts at: 0 915 1024 1485 Size: 915 109 461 Fragments arranged by size: 915 461 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1216 1485 Size: 1216 269 EcoICRI GAG'CTC Cuts at: 0 200 308 923 1485 Size: 200 108 615 562 Fragments arranged by size: 615 562 200 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 434 595 607 735 760 896 1485 Size: 163 271 161 12 128 25 136 589 Fragments arranged by size: 589 271 163 161 136 128 25 12 EcoO109I rG'GnC_Cy Cuts at: 0 216 431 582 1485 Size: 216 215 151 903 Fragments arranged by size: 903 216 215 151 HaeII r_GCGC'y Cuts at: 0 1245 1485 Size: 1245 240 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 402 434 991 1023 1429 1461 1485 Size: 402 32 557 32 406 32 24 Fragments arranged by size: 557 406 402 32 32 32 24 HincII GTy'rAC Cuts at: 0 972 1485 Size: 972 513 KpnI G_GTAC'C Cuts at: 0 551 1485 Size: 551 934 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 143 368 445 1472 1485 Size: 143 225 77 1027 13 Fragments arranged by size: 1027 225 143 77 13 MscI TGG'CCA Cuts at: 0 28 1485 Size: 28 1457 MspA1I CmG'CkG Cuts at: 0 24 80 141 246 480 533 1485 Size: 24 56 61 105 234 53 952 Fragments arranged by size: 952 234 105 61 56 53 24 NaeI GCC'GGC Cuts at: 0 513 1485 Size: 513 972 NcoI C'CATG_G Cuts at: 0 5 1485 Size: 5 1480 NgoMIV G'CCGG_C Cuts at: 0 511 1485 Size: 511 974 NspI r_CATG'y Cuts at: 0 372 1100 1281 1485 Size: 372 728 181 204 Fragments arranged by size: 728 372 204 181 PasI CC'CwG_GG Cuts at: 0 670 1485 Size: 670 815 PciI A'CATG_T Cuts at: 0 368 1277 1485 Size: 368 909 208 Fragments arranged by size: 909 368 208 PflMI CCAn_nnn'nTGG Cuts at: 0 209 585 1485 Size: 209 376 900 Fragments arranged by size: 900 376 209 PmlI CAC'GTG Cuts at: 0 1344 1485 Size: 1344 141 PpuMI rG'GwC_Cy Cuts at: 0 216 582 1485 Size: 216 366 903 Fragments arranged by size: 903 366 216 PshAI GACnn'nnGTC Cuts at: 0 221 371 1485 Size: 221 150 1114 Fragments arranged by size: 1114 221 150 PstI C_TGCA'G Cuts at: 0 352 479 1485 Size: 352 127 1006 Fragments arranged by size: 1006 352 127 PvuII CAG'CTG Cuts at: 0 80 141 480 1485 Size: 80 61 339 1005 Fragments arranged by size: 1005 339 80 61 SacI G_AGCT'C Cuts at: 0 202 310 925 1485 Size: 202 108 615 560 Fragments arranged by size: 615 560 202 108 SapI GCTCTTCn'nnn_ Cuts at: 0 915 1024 1485 Size: 915 109 461 Fragments arranged by size: 915 461 109 SfcI C'TryA_G Cuts at: 0 348 475 528 1485 Size: 348 127 53 957 Fragments arranged by size: 957 348 127 53 SmaI CCC'GGG Cuts at: 0 1012 1485 Size: 1012 473 SmlI C'TyrA_G Cuts at: 0 154 267 723 1485 Size: 154 113 456 762 Fragments arranged by size: 762 456 154 113 StuI AGG'CCT Cuts at: 0 1121 1485 Size: 1121 364 StyI C'CwwG_G Cuts at: 0 5 273 815 1485 Size: 5 268 542 670 Fragments arranged by size: 670 542 268 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 432 450 464 482 928 943 960 975 Size: 432 18 14 18 446 15 17 15 Cuts at: 975 1485 Size: 510 Fragments arranged by size: 510 446 432 18 18 17 15 15 14 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 477 1485 Size: 477 1008 XmaI C'CCGG_G Cuts at: 0 1010 1485 Size: 1010 475 XmnI GAAnn'nnTTC Cuts at: 0 1222 1409 1485 Size: 1222 187 76 Fragments arranged by size: 1222 187 76 Enzymes that do cut: AccI Acc65I AcuI AflII AflIII AlwNI ApoI AvaI BamHI BanI BanII BbsI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KpnI MmeI MscI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PmlI PpuMI PshAI PstI PvuII SacI SapI SfcI SmaI SmlI StuI StyI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI MslI NarI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI XhoI ZraI