################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbeI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MamI MluI MroI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflMI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 1183 GACGCC G[GA]^CG[CT]C AgeI 34 ACCGGT A^CCGGT AspI 1117 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 275 TGGCCA TGG^CCA BamHI 1500 GGATCC G^GATCC BbrPI 1189 CACGTG CAC^GTG BbsI 204 GAAGAC GAAGAC(2/6) BcgI 830 CGACACGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 310 CTAG C^TAG BpuAI 204 GAAGAC GAAGAC(2/6) BsaAI 1189 CACGTG [CT]AC^GT[GA] BsaHI 1183 GACGCC G[GA]^CG[CT]C BsaI 1327 GAGACC GGTCTC(1/5) BsaWI 34 ACCGGT [AT]^CCGG[AT] BsgI 1339 CTGCAC GTGCAG(16/14) BstEII 1020 GGTGACC G^GT[ACGT]ACC BstPI 1020 GGTGACC G^GT[ACGT]ACC BstXI 51 CCAGACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 1015 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1020 GGTGACC G^GT[ACGT]ACC HaeII 421 AGCGCC [GA]GCGC^[CT] Hin1I 1183 GACGCC G[GA]^CG[CT]C KspI 671 CCGCGG CCGC^GG MaeI 310 CTAG C^TAG MaeII 1190 ACGT A^CGT MfeI 533 CAATTG C^AATTG MluNI 275 TGGCCA TGG^CCA MscI 275 TGGCCA TGG^CCA MseI 815 TTAA T^TAA MunI 533 CAATTG C^AATTG NaeI 1465 GCCGGC GCC^GGC NgoMIV 1465 GCCGGC G^CCGGC PaeR7I 236 CTCGAG C^TCGAG PflFI 1117 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 34 ACCGGT A^CCGGT PleI 1489 GACTC GAGTC(4/5) PmaCI 1189 CACGTG CAC^GTG PmlI 1189 CACGTG CAC^GTG PshAI 733 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 469 GAGCTC GAGCT^C SacII 671 CCGCGG CCGC^GG ScaI 315 AGTACT AGT^ACT SexAI 1016 ACCAGGT A^CC[AT]GGT SfiI 489 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TfiI 962 GATTC G^A[AT]TC Tru9I 815 TTAA T^TAA Tth111I 1117 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 138 CCAGGATGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 236 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 660 CGCG CG^CG 672 CGCG AcsI 762 GAATTT [GA]^AATT[CT] 1147 AAATTC AhdI 930 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1243 GACTTTGGGTC Alw44I 544 GTGCAC G^TGCAC 825 GTGCAC ApaLI 544 GTGCAC G^TGCAC 825 GTGCAC ApoI 762 GAATTT [GA]^AATT[CT] 1147 AAATTC AspEI 930 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1243 GACTTTGGGTC AvaI 236 CTCGAG C^[CT]CG[GA]G 1321 CCCGAG BbvCI 385 CCTCAGC CCTCAGC(-5/-2) 558 GCTGAGG BpmI 1008 CTCCAG CTGGAG(16/14) 857 CTGGAG BsmI 614 GCATTC GAATGC(1/-1) 1174 GCATTC BsoBI 236 CTCGAG C^[CT]CG[GA]G 1321 CCCGAG BsrBI 231 CCGCTC CCGCTC(-3/-3) 406 GAGCGG BstF5I 1284 CATCC GGATG(2/0) 141 GGATG BstUI 660 CGCG CG^CG 672 CGCG BstYI 587 AGATCC [GA]^GATC[CT] 1500 GGATCC CfoI 422 GCGC GCG^C 659 GCGC DsaI 671 CCGCGG C^C[GA][CT]GG 809 CCGTGG Eam1105I 930 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1243 GACTTTGGGTC EarI 1408 CTCTTC CTCTTC(1/4) 463 GAAGAG FokI 1284 CATCC GGATG(9/13) 141 GGATG HhaI 422 GCGC GCG^C 659 GCGC HinP1I 422 GCGC G^CGC 659 GCGC HinfI 962 GATTC G^A[ACGT]TC 1489 GACTC Ksp632I 1408 CTCTTC CTCTTC(1/4) 463 GAAGAG MflI 587 AGATCC [GA]^GATC[CT] 1500 GGATCC MslI 1080 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1284 CATCCCGGTG MvnI 660 CGCG CG^CG 672 CGCG SbfI 696 CCTGCAGG CCTGCA^GG 1397 CCTGCAGG SmlI 236 CTCGAG C^T[CT][GA]AG 649 CTCAAG Sse8387I 696 CCTGCAGG CCTGCA^GG 1397 CCTGCAGG StuI 156 AGGCCT AGG^CCT 701 AGGCCT TaqI 237 TCGA T^CGA 1082 TCGA TthHB8I 237 TCGA T^CGA 1082 TCGA XhoII 587 AGATCC [GA]^GATC[CT] 1500 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaII BanI BanII BbvI BcnI BglI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstNI Cac8I Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HgaI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I Tsp509I TspRI