(Linear) (Six-base) MAPSORT of: KIEE3641.seq Check: 8520 from: 1 to: 1509 KIEE3641.orf With 182 enzymes: SgfI * December 27, 2011 16:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 531 1509 Size: 531 978 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 1509 Size: 28 1481 AgeI A'CCGG_T Cuts at: 0 34 1509 Size: 34 1475 AhdI GACnn_n'nnGTC Cuts at: 0 935 1248 1509 Size: 935 313 261 Fragments arranged by size: 935 313 261 AlwNI CAG_nnn'CTG Cuts at: 0 450 482 555 636 696 717 777 900 Size: 450 32 73 81 60 21 60 123 Cuts at: 900 927 1118 1462 1509 Size: 27 191 344 47 Fragments arranged by size: 450 344 191 123 81 73 60 60 47 32 27 21 ApaI G_GGCC'C Cuts at: 0 78 92 126 1237 1509 Size: 78 14 34 1111 272 Fragments arranged by size: 1111 272 78 34 14 ApaLI G'TGCA_C Cuts at: 0 544 825 1509 Size: 544 281 684 Fragments arranged by size: 684 544 281 ApoI r'AATT_y Cuts at: 0 762 1147 1509 Size: 762 385 362 Fragments arranged by size: 762 385 362 AvaI C'yCGr_G Cuts at: 0 236 1321 1509 Size: 236 1085 188 Fragments arranged by size: 1085 236 188 BamHI G'GATC_C Cuts at: 0 1500 1509 Size: 1500 9 BanI G'GyrC_C Cuts at: 0 249 513 1301 1509 Size: 249 264 788 208 Fragments arranged by size: 788 264 249 208 BanII G_rGCy'C Cuts at: 0 78 92 126 473 1237 1389 1509 Size: 78 14 34 347 764 152 120 Fragments arranged by size: 764 347 152 120 78 34 14 BbsI GAAGACnn'nnnn_ Cuts at: 0 211 1509 Size: 211 1298 BbvCI CC'TCA_GC Cuts at: 0 386 559 1509 Size: 386 173 950 Fragments arranged by size: 950 386 173 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 819 853 1509 Size: 819 34 656 Fragments arranged by size: 819 656 34 BglI GCCn_nnn'nGGC Cuts at: 0 328 441 496 1509 Size: 328 113 55 1013 Fragments arranged by size: 1013 328 113 55 Bme1580I G_kGCm'C Cuts at: 0 78 92 126 186 518 548 759 829 Size: 78 14 34 60 332 30 211 70 Cuts at: 829 1237 1377 1509 Size: 408 140 132 Fragments arranged by size: 408 332 211 140 132 78 70 60 34 30 14 BmrI ACTGGGnnnn_n' Cuts at: 0 993 1443 1509 Size: 993 450 66 Fragments arranged by size: 993 450 66 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 878 993 1509 Size: 878 115 516 Fragments arranged by size: 878 516 115 Bpu10I CC'TnA_GC Cuts at: 0 386 559 889 1509 Size: 386 173 330 620 Fragments arranged by size: 620 386 330 173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 1509 Size: 634 875 BsaI GGTCTCn'nnnn_ Cuts at: 0 1321 1509 Size: 1321 188 BsaAI yAC'GTr Cuts at: 0 1191 1509 Size: 1191 318 BsaHI Gr'CG_yC Cuts at: 0 1184 1509 Size: 1184 325 BsaWI w'CCGG_w Cuts at: 0 34 1509 Size: 34 1475 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 181 481 523 1339 1509 Size: 181 300 42 816 170 Fragments arranged by size: 816 300 181 170 42 BseYI C'CCAG_C Cuts at: 0 45 98 218 804 840 1509 Size: 45 53 120 586 36 669 Fragments arranged by size: 669 586 120 53 45 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1324 1509 Size: 1324 185 BsiHKAI G_wGCw'C Cuts at: 0 473 548 829 1509 Size: 473 75 281 680 Fragments arranged by size: 680 473 281 75 BsmI GAATG_Cn' Cuts at: 0 614 1174 1509 Size: 614 560 335 Fragments arranged by size: 614 560 335 Bsp1286I G_dGCh'C Cuts at: 0 78 92 126 186 473 518 548 759 Size: 78 14 34 60 287 45 30 211 Cuts at: 759 829 1237 1377 1389 1509 Size: 70 408 140 12 120 Fragments arranged by size: 408 287 211 140 120 78 70 60 45 34 30 14 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 531 644 908 1509 Size: 531 113 264 601 Fragments arranged by size: 601 531 264 113 BsrBI CCG'CTC Cuts at: 0 233 408 1509 Size: 233 175 1101 Fragments arranged by size: 1101 233 175 BsrFI r'CCGG_y Cuts at: 0 34 1304 1465 1509 Size: 34 1270 161 44 Fragments arranged by size: 1270 161 44 34 BstAPI GCAn_nnn'nTGC Cuts at: 0 696 705 1462 1509 Size: 696 9 757 47 Fragments arranged by size: 757 696 47 9 BstEII G'GTnAC_C Cuts at: 0 1020 1509 Size: 1020 489 BstXI CCAn_nnnn'nTGG Cuts at: 0 58 1509 Size: 58 1451 BstYI r'GATC_y Cuts at: 0 587 1500 1509 Size: 587 913 9 Fragments arranged by size: 913 587 9 BtgI C'CryG_G Cuts at: 0 671 809 1509 Size: 671 138 700 Fragments arranged by size: 700 671 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 638 1377 1509 Size: 638 739 132 Fragments arranged by size: 739 638 132 BtsI GCAGTG_nn' Cuts at: 0 1205 1460 1509 Size: 1205 255 49 Fragments arranged by size: 1205 255 49 DraIII CAC_nnn'GTG Cuts at: 0 1020 1509 Size: 1020 489 EaeI y'GGCC_r Cuts at: 0 275 320 578 1509 Size: 275 45 258 931 Fragments arranged by size: 931 275 258 45 EarI CTCTTCn'nnn_ Cuts at: 0 458 1414 1509 Size: 458 956 95 Fragments arranged by size: 956 458 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 403 1509 Size: 403 1106 EcoICRI GAG'CTC Cuts at: 0 471 1509 Size: 471 1038 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 878 993 1509 Size: 28 850 115 516 Fragments arranged by size: 850 516 115 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 152 163 892 1411 1509 Size: 152 11 729 519 98 Fragments arranged by size: 729 519 152 98 11 EcoO109I rG'GnC_Cy Cuts at: 0 18 74 75 82 89 552 801 864 Size: 18 56 1 7 7 463 249 63 Cuts at: 864 885 897 1140 1233 1234 1278 1404 1509 Size: 21 12 243 93 1 44 126 105 Fragments arranged by size: 463 249 243 126 105 93 63 56 44 21 18 12 7 7 1 1 HaeII r_GCGC'y Cuts at: 0 425 1509 Size: 425 1084 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 921 953 1106 1138 1234 1266 1376 1408 Size: 921 32 153 32 96 32 110 32 Cuts at: 1408 1509 Size: 101 Fragments arranged by size: 921 153 110 101 96 32 32 32 32 MfeI C'AATT_G Cuts at: 0 533 1509 Size: 533 976 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1205 1509 Size: 1205 304 MscI TGG'CCA Cuts at: 0 277 1509 Size: 277 1232 MslI CAynn'nnrTG Cuts at: 0 1084 1288 1509 Size: 1084 204 221 Fragments arranged by size: 1084 221 204 MspA1I CmG'CkG Cuts at: 0 45 98 222 390 440 526 673 774 Size: 45 53 124 168 50 86 147 101 Cuts at: 774 1102 1509 Size: 328 407 Fragments arranged by size: 407 328 168 147 124 101 86 53 50 45 NaeI GCC'GGC Cuts at: 0 1467 1509 Size: 1467 42 NgoMIV G'CCGG_C Cuts at: 0 1465 1509 Size: 1465 44 PasI CC'CwG_GG Cuts at: 0 78 1230 1509 Size: 78 1152 279 Fragments arranged by size: 1152 279 78 PfoI T'CCnGG_A Cuts at: 0 1412 1509 Size: 1412 97 PmlI CAC'GTG Cuts at: 0 1191 1509 Size: 1191 318 PpuMI rG'GwC_Cy Cuts at: 0 801 864 897 1140 1278 1404 1509 Size: 801 63 33 243 138 126 105 Fragments arranged by size: 801 243 138 126 105 63 33 PshAI GACnn'nnGTC Cuts at: 0 737 1509 Size: 737 772 PspOMI G'GGCC_C Cuts at: 0 74 88 122 1233 1509 Size: 74 14 34 1111 276 Fragments arranged by size: 1111 276 74 34 14 PstI C_TGCA'G Cuts at: 0 362 395 701 1101 1130 1402 1509 Size: 362 33 306 400 29 272 107 Fragments arranged by size: 400 362 306 272 107 33 29 PvuII CAG'CTG Cuts at: 0 45 222 390 774 1102 1509 Size: 45 177 168 384 328 407 Fragments arranged by size: 407 384 328 177 168 45 SacI G_AGCT'C Cuts at: 0 473 1509 Size: 473 1036 SacII CC_GC'GG Cuts at: 0 674 1509 Size: 674 835 SanDI GG'GwC_CC Cuts at: 0 801 864 1509 Size: 801 63 645 Fragments arranged by size: 801 645 63 SbfI CC_TGCA'GG Cuts at: 0 701 1402 1509 Size: 701 701 107 Fragments arranged by size: 701 701 107 ScaI AGT'ACT Cuts at: 0 317 1509 Size: 317 1192 SexAI A'CCwGG_T Cuts at: 0 1016 1509 Size: 1016 493 SfcI C'TryA_G Cuts at: 0 358 391 697 1097 1126 1398 1509 Size: 358 33 306 400 29 272 111 Fragments arranged by size: 400 358 306 272 111 33 29 SfiI GGCCn_nnn'nGGCC Cuts at: 0 496 1509 Size: 496 1013 SmlI C'TyrA_G Cuts at: 0 236 649 1509 Size: 236 413 860 Fragments arranged by size: 860 413 236 StuI AGG'CCT Cuts at: 0 158 703 1509 Size: 158 545 806 Fragments arranged by size: 806 545 158 StyI C'CwwG_G Cuts at: 0 491 503 1169 1509 Size: 491 12 666 340 Fragments arranged by size: 666 491 340 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 151 1243 1485 1509 Size: 151 1092 242 24 Fragments arranged by size: 1092 242 151 24 TatI w'GTAC_w Cuts at: 0 315 1509 Size: 315 1194 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 281 313 1509 Size: 281 32 1196 Fragments arranged by size: 1196 281 32 Tth111I GACn'n_nGTC Cuts at: 0 1120 1509 Size: 1120 389 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 145 1509 Size: 145 1364 XhoI C'TCGA_G Cuts at: 0 236 1509 Size: 236 1273 Enzymes that do cut: AarI AcuI AgeI AhdI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MfeI MmeI MscI MslI MspA1I NaeI NgoMIV PasI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI SbfI ScaI SexAI SfcI SfiI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaBI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MluI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SalI SapI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmaI XmnI ZraI