(Circular) (Six-base) MAPSORT of: pFN21AE2234.seq Check: 3668 from: 1 to: 5494 pFN21AE2234 5494 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2048 2048 Size: 5494 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3304 278 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2837 1937 Size: 900 4594 Acc65I G'GTAC_C Cuts at: 2815 2815 Size: 5494 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 2675 3557 4941 5209 1632 Size: 638 405 882 1384 268 1917 Fragments arranged by size: 1917 1384 882 638 405 268 AfeI AGC'GCT Cuts at: 2270 2270 Size: 5494 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5297 AflIII A'CryG_T Cuts at: 1203 4393 5370 1203 Size: 3190 977 1327 Fragments arranged by size: 3190 1327 977 AgeI A'CCGG_T Cuts at: 4325 4325 Size: 5494 AhdI GACnn_n'nnGTC Cuts at: 4223 4223 Size: 5494 AleI CACnn'nnGTG Cuts at: 1166 2484 2579 1166 Size: 1318 95 4081 Fragments arranged by size: 4081 1318 95 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2514 1184 Size: 1330 4164 AlwNI CAG_nnn'CTG Cuts at: 2653 3237 4809 2653 Size: 584 1572 3338 Fragments arranged by size: 3338 1572 584 ApaLI G'TGCA_C Cuts at: 3551 4707 3551 Size: 1156 4338 ApoI r'AATT_y Cuts at: 1493 2198 2803 3029 3181 5290 1493 Size: 705 605 226 152 2109 1697 Fragments arranged by size: 2109 1697 705 605 226 152 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2819 1870 Size: 73 876 4545 Fragments arranged by size: 4545 876 73 BamHI G'GATC_C Cuts at: 2167 2824 3191 2167 Size: 657 367 4470 Fragments arranged by size: 4470 657 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2815 4270 618 Size: 325 217 627 1028 1455 1842 Fragments arranged by size: 1842 1455 1028 627 325 217 BanII G_rGCy'C Cuts at: 1391 2037 2602 2610 2813 1391 Size: 646 565 8 203 4072 Fragments arranged by size: 4072 646 565 203 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4523 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3685 3719 1903 Size: 34 1748 34 3678 Fragments arranged by size: 3678 1748 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3386 4596 3386 Size: 1210 4284 BclI T'GATC_A Cuts at: 1548 2007 2715 1548 Size: 459 708 4327 Fragments arranged by size: 4327 708 459 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4105 136 Size: 107 122 71 631 605 2433 1525 Fragments arranged by size: 2433 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5489 5489 Size: 5494 BlpI GC'TnA_GC Cuts at: 2036 2911 2036 Size: 875 4619 Bme1580I G_kGCm'C Cuts at: 1790 3555 4711 1790 Size: 1765 1156 2573 Fragments arranged by size: 2573 1765 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3225 4183 442 Size: 416 53 83 399 24 1808 958 1753 Fragments arranged by size: 1808 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4154 1239 Size: 2915 2579 Bpu10I CC'TnA_GC Cuts at: 2604 2604 Size: 5494 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3619 4484 4782 3619 Size: 865 298 4331 Fragments arranged by size: 4331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4157 882 Size: 617 2658 2219 Fragments arranged by size: 2658 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2448 3285 493 Size: 711 1244 837 2702 Fragments arranged by size: 2702 1244 837 711 BsaBI GATnn'nnATC Cuts at: 5488 5488 Size: 5494 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3301 3683 4318 275 Size: 53 83 186 936 1768 382 635 1451 Fragments arranged by size: 1768 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3927 4325 4599 4746 5157 3927 Size: 398 274 147 411 4264 Fragments arranged by size: 4264 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 1178 Size: 30 165 30 717 30 4522 Fragments arranged by size: 4522 717 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 2742 1186 Size: 1003 553 3938 Fragments arranged by size: 3938 1003 553 BseYI C'CCAG_C Cuts at: 2029 2105 2163 2307 2502 4697 5193 2029 Size: 76 58 144 195 2195 496 2330 Fragments arranged by size: 2330 2195 496 195 144 76 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2032 1163 Size: 573 296 4625 Fragments arranged by size: 4625 573 296 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2944 3705 4733 1540 Size: 275 186 943 761 1028 2301 Fragments arranged by size: 2301 1028 943 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2592 3555 3640 4711 2592 Size: 963 85 1071 3375 Fragments arranged by size: 3375 1071 963 85 BsmI GAATG_Cn' Cuts at: 3005 3098 3005 Size: 93 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2592 2602 2610 2813 3555 3640 Size: 399 247 555 10 8 203 742 85 Cuts at: 3640 4711 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 555 399 247 203 85 10 8 BspEI T'CCGG_A Cuts at: 5157 5157 Size: 5494 BspHI T'CATG_A Cuts at: 3383 5113 3383 Size: 1730 3764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2048 2168 2849 844 Size: 1204 120 681 3489 Fragments arranged by size: 3489 1204 681 120 BsrBI CCG'CTC Cuts at: 1394 1823 3381 1394 Size: 429 1558 3507 Fragments arranged by size: 3507 1558 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2284 3989 4163 65 Size: 1183 1036 1705 174 1396 Fragments arranged by size: 1705 1396 1183 1036 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4138 4325 1700 Size: 110 2328 187 2869 Fragments arranged by size: 2869 2328 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5494 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 2383 3554 4566 2383 Size: 1171 1012 3311 Fragments arranged by size: 3311 1171 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2272 794 Size: 1478 4016 BstBI TT'CG_AA Cuts at: 4298 4298 Size: 5494 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5494 BstXI CCAn_nnnn'nTGG Cuts at: 2510 2510 Size: 5494 BstYI r'GATC_y Cuts at: 1972 2149 2167 2824 3191 3576 3593 5045 5131 Size: 177 18 657 367 385 17 1452 86 Cuts at: 5131 5143 5489 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 657 385 367 346 177 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4942 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4755 Fragments arranged by size: 4755 683 56 BtsI GCAGTG_nn' Cuts at: 2491 2679 3010 3804 3824 2491 Size: 188 331 794 20 4161 Fragments arranged by size: 4161 794 331 188 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5494 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5459 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4501 809 Size: 507 3185 1802 Fragments arranged by size: 3185 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2342 2734 2941 Size: 54 1425 50 275 18 512 392 207 Cuts at: 2941 3830 5459 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 512 392 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2941 1537 Size: 275 1129 4090 Fragments arranged by size: 4090 1129 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2760 3424 1353 Size: 1407 664 3423 Fragments arranged by size: 3423 1407 664 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2740 4081 4455 4601 387 Size: 1064 138 1151 1341 374 146 1280 Fragments arranged by size: 1341 1280 1151 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2675 3557 4154 4941 5209 1239 Size: 393 638 405 882 597 787 268 1524 Fragments arranged by size: 1524 882 787 638 597 405 393 268 EcoRI G'AATT_C Cuts at: 2803 3181 2803 Size: 378 5116 FspI TGC'GCA Cuts at: 2276 2490 4000 2276 Size: 214 1510 3770 Fragments arranged by size: 3770 1510 214 FspAI rTGC'GCAy Cuts at: 2490 2490 Size: 5494 HaeII r_GCGC'y Cuts at: 1678 2272 4641 1678 Size: 594 2369 2531 Fragments arranged by size: 2531 2369 594 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4135 4167 4209 4241 1373 Size: 32 135 32 2563 32 42 32 2626 Fragments arranged by size: 2626 2563 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2838 3084 669 Size: 988 281 900 246 3079 Fragments arranged by size: 3079 988 900 281 246 HindIII A'AGCT_T Cuts at: 748 2465 748 Size: 1717 3777 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KpnI G_GTAC'C Cuts at: 2819 2819 Size: 5494 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2076 4608 4792 1305 Size: 771 2532 184 2007 Fragments arranged by size: 2532 2007 771 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2736 10 Size: 54 1425 343 904 2768 Fragments arranged by size: 2768 1425 904 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 2484 2579 3452 3811 3970 518 Size: 648 1095 223 95 873 359 159 2042 Fragments arranged by size: 2042 1095 873 648 359 223 159 95 MspA1I CmG'CkG Cuts at: 1606 2023 2721 2910 3587 4735 4980 1606 Size: 417 698 189 677 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 417 245 189 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4942 NdeI CA'TA_TG Cuts at: 387 387 Size: 5494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2852 4397 1132 Size: 97 1623 1545 2229 Fragments arranged by size: 2229 1623 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5494 PciI A'CATG_T Cuts at: 4393 4393 Size: 5494 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PmlI CAC'GTG Cuts at: 2448 2448 Size: 5494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5076 5108 3563 Size: 32 1481 32 3949 Fragments arranged by size: 3949 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5494 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PstI C_TGCA'G Cuts at: 830 2674 2846 830 Size: 1844 172 3478 Fragments arranged by size: 3478 1844 172 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 PvuII CAG'CTG Cuts at: 2721 2721 Size: 5494 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SbfI CC_TGCA'GG Cuts at: 2846 2846 Size: 5494 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SfcI C'TryA_G Cuts at: 826 1045 2670 2842 3977 4658 4849 826 Size: 219 1625 172 1135 681 191 1471 Fragments arranged by size: 1625 1471 1135 681 219 191 172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2821 1872 Size: 949 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3598 4499 4761 820 Size: 197 926 1655 901 262 1553 Fragments arranged by size: 1655 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2852 1132 Size: 1720 3774 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5494 StyI C'CwwG_G Cuts at: 513 1065 2933 513 Size: 552 1868 3074 Fragments arranged by size: 3074 1868 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 3521 3538 3691 3876 4321 325 Size: 1255 741 1200 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1200 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3740 5452 96 Size: 274 80 33 51 1443 1763 1712 138 Fragments arranged by size: 1763 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2740 2772 5454 5486 2740 Size: 32 2682 32 2748 Fragments arranged by size: 2748 2682 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5494 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5494 XmaI C'CCGG_G Cuts at: 1870 2819 1870 Size: 949 4545 XmnI GAAnn'nnTTC Cuts at: 3623 3623 Size: 5494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3302 276 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI