################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI AocI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MseI MslI MunI NcoI NdeI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SalI SbfI ScaI SfcI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 1538 AAATTT [GA]^AATT[CT] AflIII 188 ACACGT A^C[GA][CT]GT Alw44I 297 GTGCAC G^TGCAC Aor51HI 1188 AGCGCT AGC^GCT ApaLI 297 GTGCAC G^TGCAC ApoI 1538 AAATTT [GA]^AATT[CT] AscI 351 GGCGCGCC GG^CGCGCC AspI 304 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 201 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 217 CTAG C^TAG BglI 1048 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BspHI 1289 TCATGA T^CATGA BsrBI 624 CCGCTC CCGCTC(-3/-3) BstAPI 1691 GCACGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 173 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 128 AGATCC [GA]^GATC[CT] DdeI 1736 CTAAG C^T[ACGT]AG Eco47III 1188 AGCGCT AGC^GCT EcoNI 1125 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1655 CCAAGG C^C[AT][AT]GG FseI 480 GGCCGGCC GGCCGG^CC HinfI 403 GACTC G^A[ACGT]TC MaeI 217 CTAG C^TAG MflI 128 AGATCC [GA]^GATC[CT] NheI 216 GCTAGC G^CTAGC NotI 1459 GCGGCCGC GC^GGCCGC PflFI 304 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 403 GACTC GAGTC(4/5) PshAI 1107 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1289 TCATGA T^CATGA SacI 1587 GAGCTC GAGCT^C SapI 618 GAAGAGC GCTCTTC(1/4) SfiI 1047 GGCCATCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1102 CTCAAG C^T[CT][GA]AG StuI 1658 AGGCCT AGG^CCT StyI 1655 CCAAGG C^C[AT][AT]GG Tsp509I 1539 AATT ^AATT Tth111I 304 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 128 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 826 GTCTAC GT^[AC][GT]AC 1399 GTCTAC AlwNI 40 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1612 CAGAGCCTG BalI 974 TGGCCA TGG^CCA 1169 TGGCCA BsaI 551 GAGACC GGTCTC(1/5) 309 GGTCTC BsgI 936 CTGCAC GTGCAG(16/14) 238 GTGCAG BsmAI 551 GAGAC GTCTC(1/5) 310 GTCTC BsmFI 1484 GGGAC GGGAC(10/14) 669 GTCCC BssSI 1042 CACGAG CACGAG(-5/-1) 627 CTCGTG EarI 1270 CTCTTC CTCTTC(1/4) 618 GAAGAG Ksp632I 1270 CTCTTC CTCTTC(1/4) 618 GAAGAG MaeIII 881 GTGAC ^GT[ACGT]AC 1548 GTCAC MluNI 974 TGGCCA TGG^CCA 1169 TGGCCA MscI 974 TGGCCA TGG^CCA 1169 TGGCCA NaeI 332 GCCGGC GCC^GGC 481 GCCGGC NgoMIV 332 GCCGGC G^CCGGC 481 GCCGGC NspI 167 GCATGC [GA]CATG^[CT] 512 GCATGC PflMI 117 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 606 CCAGTACGTGG PvuII 40 CAGCTG CAG^CTG 1637 CAGCTG SfaNI 1180 GATGC GCATC(5/9) 146 GCATC SphI 167 GCATGC GCATG^C 512 GCATGC Tsp45I 881 GTGAC ^GT[GC]AC 1548 GTCAC Van91I 117 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 606 CCAGTACGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AspHI AvaI AvaII AviII BanI BanII BbeI BbsI BbvI BcnI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV RsaI SacII Sau3AI Sau96I ScrFI SexAI SfoI SmaI TaqI TseI TspRI TthHB8I XmaCI XmaI