(Linear) (Six-base) MAPSORT of: KIEE1479.seq Check: 7887 from: 1 to: 1773 KIEE1479.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 269 592 1387 1773 Size: 269 323 795 386 Fragments arranged by size: 795 386 323 269 AccI GT'mk_AC Cuts at: 0 827 1400 1773 Size: 827 573 373 Fragments arranged by size: 827 573 373 AfeI AGC'GCT Cuts at: 0 1190 1773 Size: 1190 583 AflIII A'CryG_T Cuts at: 0 188 1773 Size: 188 1585 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 657 1773 Size: 657 1116 AlwNI CAG_nnn'CTG Cuts at: 0 45 1617 1773 Size: 45 1572 156 Fragments arranged by size: 1572 156 45 ApaI G_GGCC'C Cuts at: 0 562 650 1472 1650 1773 Size: 562 88 822 178 123 Fragments arranged by size: 822 562 178 123 88 ApaLI G'TGCA_C Cuts at: 0 297 1773 Size: 297 1476 ApoI r'AATT_y Cuts at: 0 1538 1773 Size: 1538 235 AscI GG'CGCG_CC Cuts at: 0 352 1773 Size: 352 1421 AvaI C'yCGr_G Cuts at: 0 466 561 787 1649 1672 1773 Size: 466 95 226 862 23 101 Fragments arranged by size: 862 466 226 101 95 23 BanI G'GyrC_C Cuts at: 0 9 462 525 580 1209 1303 1368 1427 Size: 9 453 63 55 629 94 65 59 Cuts at: 1427 1509 1773 Size: 82 264 Fragments arranged by size: 629 453 264 94 82 65 63 59 55 9 BanII G_rGCy'C Cuts at: 0 497 562 650 770 1472 1507 1591 1650 Size: 497 65 88 120 702 35 84 59 Cuts at: 1650 1773 Size: 123 Fragments arranged by size: 702 497 123 120 88 84 65 59 35 BbeI G_GCGC'C Cuts at: 0 13 466 529 1431 1773 Size: 13 453 63 902 342 Fragments arranged by size: 902 453 342 63 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 939 1300 1341 1518 1773 Size: 939 361 41 177 255 Fragments arranged by size: 939 361 255 177 41 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 190 224 1773 Size: 190 34 1549 Fragments arranged by size: 1549 190 34 BglI GCCn_nnn'nGGC Cuts at: 0 1054 1773 Size: 1054 719 Bme1580I G_kGCm'C Cuts at: 0 301 562 650 1214 1373 1472 1514 1650 Size: 301 261 88 564 159 99 42 136 Cuts at: 1650 1773 Size: 123 Fragments arranged by size: 564 301 261 159 136 123 99 88 42 BmgBI CAC'GTC Cuts at: 0 191 1687 1773 Size: 191 1496 86 Fragments arranged by size: 1496 191 86 BmrI ACTGGGnnnn_n' Cuts at: 0 1163 1773 Size: 1163 610 BmtI G_CTAG'C Cuts at: 0 220 1773 Size: 220 1553 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 784 943 1342 1773 Size: 88 696 159 399 431 Fragments arranged by size: 696 431 399 159 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1087 1773 Size: 1087 686 BsaI GGTCTCn'nnnn_ Cuts at: 0 315 545 1773 Size: 315 230 1228 Fragments arranged by size: 1228 315 230 BsaAI yAC'GTr Cuts at: 0 612 906 1167 1773 Size: 612 294 261 606 Fragments arranged by size: 612 606 294 261 BsaHI Gr'CG_yC Cuts at: 0 10 316 463 526 653 1428 1574 1773 Size: 10 306 147 63 127 775 146 199 Fragments arranged by size: 775 306 199 147 146 127 63 10 BseYI C'CCAG_C Cuts at: 0 180 1233 1773 Size: 180 1053 540 Fragments arranged by size: 1053 540 180 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 921 1773 Size: 259 662 852 Fragments arranged by size: 852 662 259 BsiEI CG_ry'CG Cuts at: 0 78 482 1463 1773 Size: 78 404 981 310 Fragments arranged by size: 981 404 310 78 BsiHKAI G_wGCw'C Cuts at: 0 280 301 752 1591 1773 Size: 280 21 451 839 182 Fragments arranged by size: 839 451 280 182 21 Bsp1286I G_dGCh'C Cuts at: 0 280 301 497 562 650 752 770 1214 Size: 280 21 196 65 88 102 18 444 Cuts at: 1214 1373 1472 1507 1514 1591 1650 1773 Size: 159 99 35 7 77 59 123 Fragments arranged by size: 444 280 196 159 123 102 99 88 77 65 59 35 21 18 7 BspHI T'CATG_A Cuts at: 0 1289 1773 Size: 1289 484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 185 269 592 1387 1773 Size: 185 84 323 795 386 Fragments arranged by size: 795 386 323 185 84 BsrBI CCG'CTC Cuts at: 0 626 1773 Size: 626 1147 BsrFI r'CCGG_y Cuts at: 0 332 481 1255 1773 Size: 332 149 774 518 Fragments arranged by size: 774 518 332 149 BssHII G'CGCG_C Cuts at: 0 352 365 538 896 1773 Size: 352 13 173 358 877 Fragments arranged by size: 877 358 352 173 13 BssSI C'ACGA_G Cuts at: 0 627 1042 1773 Size: 627 415 731 Fragments arranged by size: 731 627 415 BstAPI GCAn_nnn'nTGC Cuts at: 0 1697 1773 Size: 1697 76 BstXI CCAn_nnnn'nTGG Cuts at: 0 180 1773 Size: 180 1593 BstYI r'GATC_y Cuts at: 0 128 1773 Size: 128 1645 BtgI C'CryG_G Cuts at: 0 5 141 476 488 521 529 1059 1463 Size: 5 136 335 12 33 8 530 404 Cuts at: 1463 1517 1718 1773 Size: 54 201 55 Fragments arranged by size: 530 404 335 201 136 55 54 33 12 8 5 BtsI GCAGTG_nn' Cuts at: 0 582 1695 1773 Size: 582 1113 78 Fragments arranged by size: 1113 582 78 EaeI y'GGCC_r Cuts at: 0 2 75 397 479 704 857 974 1169 Size: 2 73 322 82 225 153 117 195 Cuts at: 1169 1460 1641 1773 Size: 291 181 132 Fragments arranged by size: 322 291 225 195 181 153 132 117 82 73 2 EagI C'GGCC_G Cuts at: 0 75 479 1460 1773 Size: 75 404 981 313 Fragments arranged by size: 981 404 313 75 EarI CTCTTCn'nnn_ Cuts at: 0 613 1276 1773 Size: 613 663 497 Fragments arranged by size: 663 613 497 EcoICRI GAG'CTC Cuts at: 0 1589 1773 Size: 1589 184 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 784 943 1342 1773 Size: 88 696 159 399 431 Fragments arranged by size: 696 431 399 159 88 EcoNI CCTnn'n_nnAGG Cuts at: 0 1129 1773 Size: 1129 644 EcoO109I rG'GnC_Cy Cuts at: 0 504 918 1089 1468 1773 Size: 504 414 171 379 305 Fragments arranged by size: 504 414 379 305 171 FseI GG_CCGG'CC Cuts at: 0 485 1773 Size: 485 1288 FspI TGC'GCA Cuts at: 0 633 1129 1455 1773 Size: 633 496 326 318 Fragments arranged by size: 633 496 326 318 FspAI rTGC'GCAy Cuts at: 0 633 1773 Size: 633 1140 HaeII r_GCGC'y Cuts at: 0 13 28 466 529 721 795 1192 1431 Size: 13 15 438 63 192 74 397 239 Cuts at: 1431 1773 Size: 342 Fragments arranged by size: 438 397 342 239 192 74 63 15 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 295 327 1773 Size: 295 32 1446 Fragments arranged by size: 1446 295 32 KasI G'GCGC_C Cuts at: 0 9 462 525 1427 1773 Size: 9 453 63 902 346 Fragments arranged by size: 902 453 346 63 9 MscI TGG'CCA Cuts at: 0 976 1171 1773 Size: 976 195 602 Fragments arranged by size: 976 602 195 MspA1I CmG'CkG Cuts at: 0 42 143 234 490 531 1459 1465 1639 Size: 42 101 91 256 41 928 6 174 Cuts at: 1639 1773 Size: 134 Fragments arranged by size: 928 256 174 134 101 91 42 41 6 NaeI GCC'GGC Cuts at: 0 334 483 1773 Size: 334 149 1290 Fragments arranged by size: 1290 334 149 NarI GG'CG_CC Cuts at: 0 10 463 526 1428 1773 Size: 10 453 63 902 345 Fragments arranged by size: 902 453 345 63 10 NgoMIV G'CCGG_C Cuts at: 0 332 481 1773 Size: 332 149 1292 Fragments arranged by size: 1292 332 149 NheI G'CTAG_C Cuts at: 0 216 1773 Size: 216 1557 NotI GC'GGCC_GC Cuts at: 0 1460 1773 Size: 1460 313 NspI r_CATG'y Cuts at: 0 171 516 1773 Size: 171 345 1257 Fragments arranged by size: 1257 345 171 PflMI CCAn_nnn'nTGG Cuts at: 0 123 612 1773 Size: 123 489 1161 Fragments arranged by size: 1161 489 123 PfoI T'CCnGG_A Cuts at: 0 546 911 1773 Size: 546 365 862 Fragments arranged by size: 862 546 365 PshAI GACnn'nnGTC Cuts at: 0 1111 1773 Size: 1111 662 PspOMI G'GGCC_C Cuts at: 0 558 646 1468 1646 1773 Size: 558 88 822 178 127 Fragments arranged by size: 822 558 178 127 88 PvuII CAG'CTG Cuts at: 0 42 1639 1773 Size: 42 1597 134 Fragments arranged by size: 1597 134 42 SacI G_AGCT'C Cuts at: 0 1591 1773 Size: 1591 182 SacII CC_GC'GG Cuts at: 0 144 491 532 1466 1773 Size: 144 347 41 934 307 Fragments arranged by size: 934 347 307 144 41 SapI GCTCTTCn'nnn_ Cuts at: 0 613 1773 Size: 613 1160 SexAI A'CCwGG_T Cuts at: 0 305 1009 1226 1773 Size: 305 704 217 547 Fragments arranged by size: 704 547 305 217 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1054 1773 Size: 1054 719 SfoI GGC'GCC Cuts at: 0 11 464 527 1429 1773 Size: 11 453 63 902 344 Fragments arranged by size: 902 453 344 63 11 SmaI CCC'GGG Cuts at: 0 563 1651 1674 1773 Size: 563 1088 23 99 Fragments arranged by size: 1088 563 99 23 SmlI C'TyrA_G Cuts at: 0 1102 1773 Size: 1102 671 SphI G_CATG'C Cuts at: 0 171 516 1773 Size: 171 345 1257 Fragments arranged by size: 1257 345 171 SrfI GCCC'GGGC Cuts at: 0 563 1651 1773 Size: 563 1088 122 Fragments arranged by size: 1088 563 122 StuI AGG'CCT Cuts at: 0 1660 1773 Size: 1660 113 StyI C'CwwG_G Cuts at: 0 1655 1773 Size: 1655 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 981 1569 1773 Size: 981 588 204 Fragments arranged by size: 981 588 204 Tth111I GACn'n_nGTC Cuts at: 0 307 1773 Size: 307 1466 XmaI C'CCGG_G Cuts at: 0 561 1649 1672 1773 Size: 561 1088 23 101 Fragments arranged by size: 1088 561 101 23 Enzymes that do cut: AarI AccI AfeI AflIII AlfI AlwNI ApaI ApaLI ApoI AscI AvaI BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI FspAI HaeII Hin4I KasI MscI MspA1I NaeI NarI NgoMIV NheI NotI NspI PflMI PfoI PshAI PspOMI PvuII SacI SacII SapI SexAI SfiI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII Tth111I XmaI Enzymes that do not cut: AatII Acc65I AclI AcuI AflII AgeI AhdI AleI AloI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI BplI Bpu10I BsaBI BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI HincII HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI NdeI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SnaBI SpeI SspI SwaI TatI TstI XbaI XcmI XhoI XmnI ZraI