################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MflI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI Tth111I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 348 GACGTC GACGT^C AlwNI 739 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 1090 GAGCTC G[AT]GC[AT]^C AvaI 254 CTCGGG C^[CT]CG[GA]G BanI 1077 GGTGCC G^G[CT][GA]CC BbsI 688 GAAGAC GAAGAC(2/6) BclI 809 TGATCA T^GATCA BcnI 24 CCGGG CC^[GC]GG BpuAI 688 GAAGAC GAAGAC(2/6) BsiEI 109 CGGTCG CG[GA][CT]^CG BsiHKAI 1090 GAGCTC G[AT]GC[AT]^C BsmAI 126 GTCTC GTCTC(1/5) BsoBI 254 CTCGGG C^[CT]CG[GA]G BsrBI 67 CCGCTC CCGCTC(-3/-3) BstXI 1190 CCAACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 825 GCGC GCG^C CpoI 20 CGGACCG CG^G[AT]CCG DraI 1273 TTTAAA TTT^AAA EcoRV 1051 GATATC GAT^ATC FauI 66 CCCGC CCCGC(4/6) FbaI 809 TGATCA T^GATCA HhaI 825 GCGC GCG^C HinP1I 825 GCGC G^CGC MfeI 213 CAATTG C^AATTG MslI 1008 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 895 CAGCTG C[AC]G^C[GT]G MunI 213 CAATTG C^AATTG NciI 24 CCGGG CC^[GC]GG PstI 13 CTGCAG CTGCA^G PvuII 895 CAGCTG CAG^CTG RsrII 20 CGGACCG CG^G[AT]CCG SacI 1090 GAGCTC GAGCT^C SmlI 1145 CTCAAG C^T[CT][GA]AG SphI 236 GCATGC GCATG^C SspI 446 AATATT AAT^ATT TaqI 1025 TCGA T^CGA TfiI 929 GAATC G^A[AT]TC TspRI 1062 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 1025 TCGA T^CGA XbaI 352 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 3 GGCGTC G[GA]^CG[CT]C 348 GACGTC AfaI 593 GTAC GT^AC 1125 GTAC AlwI 1162 GATCC GGATC(4/5) 627 GGATC ApaI 62 GGGCCC GGGCC^C 1365 GGGCCC BsaHI 3 GGCGTC G[GA]^CG[CT]C 348 GACGTC BsaJI 42 CCAGGG C^C[ACGT][ACGT]GG 1317 CCAGGG BseRI 1033 GAGGAG GAGGAG(10/8) 1231 GAGGAG BsiYI 571 CCACGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1344 CCAGCATGTGG BslI 571 CCACGAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1344 CCAGCATGTGG DraIII 130 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG 1223 CACAGCGTG HapII 24 CCGG C^CGG 602 CCGG HgaI 4 GCGTC GACGC(5/10) 741 GCGTC Hin1I 3 GGCGTC G[GA]^CG[CT]C 348 GACGTC HpaII 24 CCGG C^CGG 602 CCGG MspI 24 CCGG C^CGG 602 CCGG MwoI 92 GCTATTGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1338 GCTCACCCAGC PflMI 571 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1344 CCAGCATGTGG PpuMI 971 AGGTCCT [GA]G^G[AT]CC[CT] 1040 GGGACCC RsaI 593 GTAC GT^AC 1125 GTAC SfcI 13 CTGCAG C^T[GA][CT]AG 380 CTGTAG Van91I 571 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1344 CCAGCATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AluI ApoI AvaII BanII BbvI BfaI BmyI BsmFI Bsp1286I BsrI BssKI BssSI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I