(Circular) (Six-base) MAPSORT of: pFN21AE6431.seq Check: 7465 from: 1 to: 6085 pFN21AE6431 6085 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2357 3895 278 Size: 53 83 186 936 821 1538 2468 Fragments arranged by size: 2468 1538 936 821 186 83 53 AccI GT'mk_AC Cuts at: 1937 3428 1937 Size: 1491 4594 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2208 2552 2712 2723 3169 4148 5532 5800 Size: 576 344 160 11 446 979 1384 268 Cuts at: 5800 1632 Size: 1917 Fragments arranged by size: 1917 1384 979 576 446 344 268 160 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 4984 5961 1203 Size: 3781 977 1327 Fragments arranged by size: 3781 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 4814 4814 Size: 6085 AleI CACnn'nnGTG Cuts at: 1166 3017 1166 Size: 1851 4234 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6085 AlwNI CAG_nnn'CTG Cuts at: 2749 3828 5400 2749 Size: 1079 1572 3434 Fragments arranged by size: 3434 1572 1079 ApaI G_GGCC'C Cuts at: 2071 3374 2071 Size: 1303 4782 ApaLI G'TGCA_C Cuts at: 4142 5298 4142 Size: 1156 4929 ApoI r'AATT_y Cuts at: 1493 2716 2867 2888 3394 3620 3772 5881 1493 Size: 1223 151 21 506 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 506 226 152 151 21 AseI AT'TA_AT Cuts at: 160 1032 4639 160 Size: 872 3607 1606 Fragments arranged by size: 3607 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 2259 3410 1870 Size: 73 316 1151 4545 Fragments arranged by size: 4545 1151 316 73 BamHI G'GATC_C Cuts at: 3415 3782 3415 Size: 367 5718 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3082 3406 4861 618 Size: 325 217 627 1295 324 1455 1842 Fragments arranged by size: 1842 1455 1295 627 325 324 217 BanII G_rGCy'C Cuts at: 1391 2071 3099 3374 3404 1391 Size: 680 1028 275 30 4072 Fragments arranged by size: 4072 1028 680 275 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2700 928 Size: 971 801 4313 Fragments arranged by size: 4313 971 801 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4276 4310 1903 Size: 34 2339 34 3678 Fragments arranged by size: 3678 2339 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5187 3977 Size: 1210 4875 BclI T'GATC_A Cuts at: 1548 2814 1548 Size: 1266 4819 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4696 136 Size: 107 122 71 631 605 3024 1525 Fragments arranged by size: 3024 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6080 6080 Size: 6085 BlpI GC'TnA_GC Cuts at: 3502 3502 Size: 6085 Bme1580I G_kGCm'C Cuts at: 1790 2071 3374 4146 5302 1790 Size: 281 1303 772 1156 2573 Fragments arranged by size: 2573 1303 1156 772 281 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2154 3816 4774 Size: 416 53 83 399 24 737 1662 958 Cuts at: 4774 442 Size: 1753 Fragments arranged by size: 1753 1662 958 737 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6085 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2161 2161 Size: 6085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4745 1239 Size: 3506 2579 Bpu10I CC'TnA_GC Cuts at: 2661 3072 2661 Size: 411 5674 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3135 4210 5075 5373 3135 Size: 1075 865 298 3847 Fragments arranged by size: 3847 1075 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4748 882 Size: 617 3249 2219 Fragments arranged by size: 3249 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3876 493 Size: 711 2672 2702 Fragments arranged by size: 2702 2672 711 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2354 3892 4274 Size: 53 83 186 936 476 345 1538 382 Cuts at: 4274 4909 275 Size: 635 1451 Fragments arranged by size: 1538 1451 936 635 476 382 345 186 83 53 BsaWI w'CCGG_w Cuts at: 4518 4916 5190 5337 5748 4518 Size: 398 274 147 411 4855 Fragments arranged by size: 4855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5860 Fragments arranged by size: 5860 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3053 3251 1186 Size: 1867 198 4020 Fragments arranged by size: 4020 1867 198 BseYI C'CCAG_C Cuts at: 2296 3348 5288 5784 2296 Size: 1052 1940 496 2597 Fragments arranged by size: 2597 1940 1052 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5512 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2117 3535 4296 5324 1540 Size: 275 186 116 1418 761 1028 2301 Fragments arranged by size: 2301 1418 1028 761 275 186 116 BsiHKAI G_wGCw'C Cuts at: 3099 4146 4231 5302 3099 Size: 1047 85 1071 3882 Fragments arranged by size: 3882 1071 1047 85 BsmI GAATG_Cn' Cuts at: 3596 3689 3596 Size: 93 5992 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 3099 3374 3404 4146 4231 5302 Size: 399 281 1028 275 30 742 85 1071 Cuts at: 5302 1391 Size: 2174 Fragments arranged by size: 2174 1071 1028 742 399 281 275 85 30 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 3974 5704 3974 Size: 1730 4355 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3440 844 Size: 2596 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2074 3972 1394 Size: 429 251 1898 3507 Fragments arranged by size: 3507 1898 429 251 BsrDI GCAATG_nn' Cuts at: 65 1248 4580 4754 65 Size: 1183 3332 174 1396 Fragments arranged by size: 3332 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4729 4916 1700 Size: 110 2919 187 2869 Fragments arranged by size: 2919 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 2135 2266 2577 4145 5157 2135 Size: 131 311 1568 1012 3063 Fragments arranged by size: 3063 1568 1012 311 131 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6085 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6085 BstXI CCAn_nnnn'nTGG Cuts at: 3202 3202 Size: 6085 BstYI r'GATC_y Cuts at: 1972 3415 3782 4167 4184 5636 5722 5734 6080 Size: 1443 367 385 17 1452 86 12 346 Cuts at: 6080 1972 Size: 1977 Fragments arranged by size: 1977 1452 1443 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4594 Fragments arranged by size: 4594 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5346 Fragments arranged by size: 5346 683 56 BtsI GCAGTG_nn' Cuts at: 3066 3601 4395 4415 3066 Size: 535 794 20 4736 Fragments arranged by size: 4736 794 535 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6085 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6050 DraI TTT'AAA Cuts at: 3280 3389 3280 Size: 109 5976 DraIII CAC_nnn'GTG Cuts at: 2140 3233 2140 Size: 1093 4992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5092 809 Size: 507 3776 1802 Fragments arranged by size: 3776 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3532 4421 6050 Size: 54 1425 50 275 18 1702 889 1629 Cuts at: 6050 8 Size: 43 Fragments arranged by size: 1702 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3532 1537 Size: 275 1720 4090 Fragments arranged by size: 4090 1720 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4015 1353 Size: 2662 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4672 5046 5192 387 Size: 1064 138 3083 374 146 1280 Fragments arranged by size: 3083 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3097 3097 Size: 6085 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2208 2552 2712 2723 3169 4148 4745 Size: 393 576 344 160 11 446 979 597 Cuts at: 4745 5532 5800 1239 Size: 787 268 1524 Fragments arranged by size: 1524 979 787 597 576 446 393 344 268 160 11 EcoO109I rG'GnC_Cy Cuts at: 2068 2977 3046 2068 Size: 909 69 5107 Fragments arranged by size: 5107 909 69 EcoRI G'AATT_C Cuts at: 3394 3772 3394 Size: 378 5707 EcoRV GAT'ATC Cuts at: 3058 3058 Size: 6085 FspI TGC'GCA Cuts at: 4591 4591 Size: 6085 HaeII r_GCGC'y Cuts at: 1678 5232 1678 Size: 3554 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4726 4758 4800 4832 1373 Size: 32 135 32 3154 32 42 32 2626 Fragments arranged by size: 3154 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3429 3675 669 Size: 988 281 1491 246 3079 Fragments arranged by size: 3079 1491 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6085 HpaI GTT'AAC Cuts at: 3675 3675 Size: 6085 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 2218 3684 2218 Size: 1466 4619 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2004 5199 5383 1305 Size: 699 3195 184 2007 Fragments arranged by size: 3195 2007 699 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4263 Fragments arranged by size: 4263 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3017 4043 4402 4561 518 Size: 648 1851 1026 359 159 2042 Fragments arranged by size: 2042 1851 1026 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2902 3501 4178 5326 5571 1606 Size: 1296 599 677 1148 245 2120 Fragments arranged by size: 2120 1296 1148 677 599 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4594 Fragments arranged by size: 4594 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6085 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6085 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 2245 2337 3356 3443 4988 1132 Size: 97 1016 92 1019 87 1545 2229 Fragments arranged by size: 2229 1545 1019 1016 97 92 87 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6085 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 2582 3355 1108 Size: 1474 773 3838 Fragments arranged by size: 3838 1474 773 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2977 3046 2977 Size: 69 6016 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6085 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PspOMI G'GGCC_C Cuts at: 2067 3370 2067 Size: 1303 4782 PstI C_TGCA'G Cuts at: 830 2022 3437 830 Size: 1192 1415 3478 Fragments arranged by size: 3478 1415 1192 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 PvuII CAG'CTG Cuts at: 2902 2902 Size: 6085 RsrII CG'GwC_CG Cuts at: 2026 2026 Size: 6085 SacI G_AGCT'C Cuts at: 3099 3099 Size: 6085 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SanDI GG'GwC_CC Cuts at: 3046 3046 Size: 6085 SbfI CC_TGCA'GG Cuts at: 3437 3437 Size: 6085 ScaI AGT'ACT Cuts at: 4333 6045 4333 Size: 1712 4373 SfcI C'TryA_G Cuts at: 826 1045 2018 2385 3433 4568 5249 5440 826 Size: 219 973 367 1048 1135 681 191 1471 Fragments arranged by size: 1471 1135 1048 973 681 367 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 3412 1872 Size: 1540 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3150 4189 5090 5352 820 Size: 197 926 1207 1039 901 262 1553 Fragments arranged by size: 1553 1207 1039 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3876 493 Size: 3383 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 2245 3443 1132 Size: 1113 1198 3774 Fragments arranged by size: 3774 1198 1113 SspI AAT'ATT Cuts at: 5 52 2453 4009 5 Size: 47 2401 1556 2081 Fragments arranged by size: 2401 2081 1556 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6085 StyI C'CwwG_G Cuts at: 513 1065 2004 3524 513 Size: 552 939 1520 3074 Fragments arranged by size: 3074 1520 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3029 3362 4112 4129 4282 4467 4912 Size: 1255 1449 333 750 17 153 185 445 Cuts at: 4912 325 Size: 1498 Fragments arranged by size: 1498 1449 1255 750 445 333 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4331 6043 96 Size: 274 80 33 51 1443 2354 1712 138 Fragments arranged by size: 2354 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6045 6077 6045 Size: 32 6053 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6085 XbaI T'CTAG_A Cuts at: 2357 3421 2357 Size: 1064 5021 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 3410 1870 Size: 1540 4545 XmnI GAAnn'nnTTC Cuts at: 4214 4214 Size: 6085 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2355 3893 276 Size: 53 83 186 936 821 1538 2468 Fragments arranged by size: 2468 1538 936 821 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI