################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BciVI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BssHII BstAPI Bsu36I CelII ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoNI EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 492 GTATAC GT^[AC][GT]AC Asp700I 1491 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1615 GTGCTC G[AT]GC[AT]^C BbvCI 1246 GCTGAGG CCTCAGC(-5/-2) BfaI 17 CTAG C^TAG BglII 1091 AGATCT A^GATCT BsaAI 1472 TACGTA [CT]AC^GT[GA] BsaI 1081 GAGACC GGTCTC(1/5) BsaWI 283 TCCGGT [AT]^CCGG[AT] BsiHKAI 1615 GTGCTC G[AT]GC[AT]^C BsmBI 450 CGTCTC CGTCTC(1/5) Bsp1407I 788 TGTACA T^GTACA BspMI 693 GCAGGT ACCTGC(4/8) BsrGI 788 TGTACA T^GTACA BssSI 1338 CACGAG CACGAG(-5/-1) Bst1107I 492 GTATAC GTA^TAC BstBI 799 TTCGAA TT^CGAA BstEII 162 GGTCACC G^GT[ACGT]ACC BstPI 162 GGTCACC G^GT[ACGT]ACC BstZ17I 492 GTATAC GTA^TAC DraIII 165 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 432 GACAGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 427 CGGCCG C^GGCCG EarI 1402 GAAGAG CTCTTC(1/4) EclXI 427 CGGCCG C^GGCCG Eco52I 427 CGGCCG C^GGCCG EcoO65I 162 GGTCACC G^GT[ACGT]ACC EcoRI 1006 GAATTC G^AATTC HaeII 1533 AGCGCC [GA]GCGC^[CT] HincII 808 GTCAAC GT[CT]^[GA]AC HindII 808 GTCAAC GT[CT]^[GA]AC Ksp632I 1402 GAAGAG CTCTTC(1/4) MaeI 17 CTAG C^TAG MspA1I 481 CAGCTG C[AC]G^C[GT]G NcoI 1124 CCATGG C^CATGG NspV 799 TTCGAA TT^CGAA PflMI 652 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 481 CAGCTG CAG^CTG ScaI 902 AGTACT AGT^ACT SfuI 799 TTCGAA TT^CGAA SnaBI 1472 TACGTA TAC^GTA SspBI 788 TGTACA T^GTACA StuI 1106 AGGCCT AGG^CCT TfiI 486 GAATC G^A[AT]TC Van91I 652 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1491 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 206 CGCG CG^CG 1023 CGCG AcsI 1006 GAATTC [GA]^AATT[CT] 1222 GAATTT ApaI 681 GGGCCC GGGCC^C 817 GGGCCC ApoI 1006 GAATTC [GA]^AATT[CT] 1222 GAATTT BalI 231 TGGCCA TGG^CCA 1155 TGGCCA BcgI 1063 GCACCCGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1382 GCACGCTGGTCG BclI 887 TGATCA T^GATCA 1265 TGATCA BglI 420 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1054 GCCTTCATGGC BsiEI 285 CGGTCG CG[GA][CT]^CG 427 CGGCCG BsrBI 307 GAGCGG CCGCTC(-3/-3) 1177 GAGCGG BsrDI 574 GCAATG GCAATG(2/0) 1001 GCAATG BsrI 804 ACTGG ACTGG(1/-1) 1158 CCAGT BstUI 206 CGCG CG^CG 1023 CGCG BstXI 947 CCAACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1034 CCAACACCGTGG BstYI 882 GGATCT [GA]^GATC[CT] 1091 AGATCT FbaI 887 TGATCA T^GATCA 1265 TGATCA HgaI 356 GACGC GACGC(5/10) 1021 GACGC MflI 882 GGATCT [GA]^GATC[CT] 1091 AGATCT MluNI 231 TGGCCA TGG^CCA 1155 TGGCCA MscI 231 TGGCCA TGG^CCA 1155 TGGCCA MvnI 206 CGCG CG^CG 1023 CGCG NaeI 613 GCCGGC GCC^GGC 1508 GCCGGC NgoMIV 613 GCCGGC G^CCGGC 1508 GCCGGC SmlI 126 CTTGAG C^T[CT][GA]AG 718 CTCAAG XhoII 882 GGATCT [GA]^GATC[CT] 1091 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I