(Circular) (Six-base) MAPSORT of: pFN21AE3630.seq Check: 7238 from: 1 to: 6328 pFN21AE3630 6328 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2689 2689 Size: 6328 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4138 278 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2498 3671 1937 Size: 561 1173 4594 Fragments arranged by size: 4594 1173 561 Acc65I G'GTAC_C Cuts at: 3649 3649 Size: 6328 AclI AA'CG_TT Cuts at: 1563 4455 4828 1563 Size: 2892 373 3063 Fragments arranged by size: 3063 2892 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 2880 3426 4391 5775 6043 1632 Size: 893 355 546 965 1384 268 1917 Fragments arranged by size: 1917 1384 965 893 546 355 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6131 AflIII A'CryG_T Cuts at: 1203 5227 6204 1203 Size: 4024 977 1327 Fragments arranged by size: 4024 1327 977 AgeI A'CCGG_T Cuts at: 5159 5159 Size: 6328 AhdI GACnn_n'nnGTC Cuts at: 5057 5057 Size: 6328 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6328 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6328 AlwNI CAG_nnn'CTG Cuts at: 2663 2890 3223 3542 4071 5643 2663 Size: 227 333 319 529 1572 3348 Fragments arranged by size: 3348 1572 529 333 319 227 ApaI G_GGCC'C Cuts at: 2690 2826 2690 Size: 136 6192 ApaLI G'TGCA_C Cuts at: 4385 5541 4385 Size: 1156 5172 ApoI r'AATT_y Cuts at: 1493 3011 3227 3637 3863 4015 6124 1493 Size: 1518 216 410 226 152 2109 1697 Fragments arranged by size: 2109 1697 1518 410 226 216 152 AseI AT'TA_AT Cuts at: 160 1032 4882 160 Size: 872 3850 1606 Fragments arranged by size: 3850 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 AvaI C'yCGr_G Cuts at: 1870 1943 2643 3071 3296 3653 1870 Size: 73 700 428 225 357 4545 Fragments arranged by size: 4545 700 428 357 225 73 BamHI G'GATC_C Cuts at: 3658 4025 3658 Size: 367 5961 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2648 3067 3313 3587 3649 Size: 325 217 627 861 419 246 274 62 Cuts at: 3649 5104 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 861 627 419 325 274 246 217 62 BanII G_rGCy'C Cuts at: 1391 2528 2690 2826 3647 1391 Size: 1137 162 136 821 4072 Fragments arranged by size: 4072 1137 821 162 136 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2868 3473 3579 928 Size: 971 969 605 106 3677 Fragments arranged by size: 3677 971 969 605 106 BbvCI CC'TCA_GC Cuts at: 3252 3252 Size: 6328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3057 3091 3376 3410 4519 4553 1903 Size: 34 1120 34 285 34 1109 34 3678 Fragments arranged by size: 3678 1120 1109 285 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4220 5430 4220 Size: 1210 5118 BclI T'GATC_A Cuts at: 1548 2892 3270 1548 Size: 1344 378 4606 Fragments arranged by size: 4606 1344 378 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2431 3065 4939 Size: 107 122 71 631 605 759 634 1874 Cuts at: 4939 136 Size: 1525 Fragments arranged by size: 1874 1525 759 634 631 605 122 107 71 BglII A'GATC_T Cuts at: 3096 6323 3096 Size: 3227 3101 BlpI GC'TnA_GC Cuts at: 3745 3745 Size: 6328 Bme1580I G_kGCm'C Cuts at: 1790 2690 2826 2841 2979 3053 3170 3590 4389 Size: 900 136 15 138 74 117 420 799 Cuts at: 4389 5545 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 900 799 420 138 136 117 74 15 BmgBI CAC'GTC Cuts at: 1318 2455 2630 1318 Size: 1137 175 5016 Fragments arranged by size: 5016 1137 175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4059 5017 442 Size: 416 53 83 399 24 2642 958 1753 Fragments arranged by size: 2642 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6328 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3097 3544 3097 Size: 447 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 2685 3566 4988 1239 Size: 1007 439 881 1422 2579 Fragments arranged by size: 2579 1422 1007 881 439 Bpu10I CC'TnA_GC Cuts at: 3252 3252 Size: 6328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2152 2708 4453 5318 5616 2152 Size: 556 1745 865 298 2864 Fragments arranged by size: 2864 1745 865 556 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3080 4991 882 Size: 617 1581 1911 2219 Fragments arranged by size: 2219 1911 1581 617 BsaAI yAC'GTr Cuts at: 493 1204 3479 4119 493 Size: 711 2275 640 2702 Fragments arranged by size: 2702 2275 711 640 BsaBI GATnn'nnATC Cuts at: 6322 6322 Size: 6328 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4135 4517 5152 275 Size: 53 83 186 936 2602 382 635 1451 Fragments arranged by size: 2602 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2288 4761 5159 5433 5580 5991 2288 Size: 2473 398 274 147 411 2625 Fragments arranged by size: 2625 2473 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 1178 Size: 30 165 30 1038 30 5035 Fragments arranged by size: 5035 1038 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2248 3023 3425 3626 1186 Size: 1062 775 402 201 3888 Fragments arranged by size: 3888 1062 775 402 201 BseYI C'CCAG_C Cuts at: 2030 2062 3440 5531 6027 2030 Size: 32 1378 2091 496 2331 Fragments arranged by size: 2331 2091 1378 496 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2504 3314 3370 1163 Size: 573 768 810 56 4121 Fragments arranged by size: 4121 810 768 573 56 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2293 2435 3778 4539 5567 1540 Size: 275 186 292 142 1343 761 1028 2301 Fragments arranged by size: 2301 1343 1028 761 292 275 186 142 BsiHKAI G_wGCw'C Cuts at: 3624 4389 4474 5545 3624 Size: 765 85 1071 4407 Fragments arranged by size: 4407 1071 765 85 BsmI GAATG_Cn' Cuts at: 3839 3932 3839 Size: 93 6235 BsmBI CGTCTCn'nnnn_ Cuts at: 2461 2461 Size: 6328 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2528 2690 2826 2841 2979 3053 3170 Size: 399 738 162 136 15 138 74 117 Cuts at: 3170 3590 3624 3647 4389 4474 5545 1391 Size: 420 34 23 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 738 420 399 162 138 136 117 85 74 34 23 15 BspEI T'CCGG_A Cuts at: 5991 5991 Size: 6328 BspHI T'CATG_A Cuts at: 4217 5947 4217 Size: 1730 4598 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2689 3683 844 Size: 1845 994 3489 Fragments arranged by size: 3489 1845 994 BsrBI CCG'CTC Cuts at: 1394 1823 2314 3184 4215 1394 Size: 429 491 870 1031 3507 Fragments arranged by size: 3507 1031 870 491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2586 3013 4823 4997 65 Size: 1183 1338 427 1810 174 1396 Fragments arranged by size: 1810 1396 1338 1183 427 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2217 2336 2618 3513 4972 5159 1700 Size: 110 407 119 282 895 1459 187 2869 Fragments arranged by size: 2869 1459 895 407 282 187 119 110 BsrGI T'GTAC_A Cuts at: 96 2793 96 Size: 2697 3631 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6328 BssSI C'ACGA_G Cuts at: 3343 4388 5400 3343 Size: 1045 1012 4271 Fragments arranged by size: 4271 1045 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6328 BstBI TT'CG_AA Cuts at: 2805 5132 2805 Size: 2327 4001 BstEII G'GTnAC_C Cuts at: 1184 2167 1184 Size: 983 5345 BstXI CCAn_nnnn'nTGG Cuts at: 2959 3046 2959 Size: 87 6241 BstYI r'GATC_y Cuts at: 1972 2887 3096 3658 4025 4410 4427 5879 5965 Size: 915 209 562 367 385 17 1452 86 Cuts at: 5965 5977 6323 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 915 562 385 367 346 209 86 17 12 BstZ17I GTA'TAC Cuts at: 2499 2499 Size: 6328 BtgI C'CryG_G Cuts at: 513 1065 3045 3129 3603 513 Size: 552 1980 84 474 3238 Fragments arranged by size: 3238 1980 552 474 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5589 Fragments arranged by size: 5589 683 56 BtsI GCAGTG_nn' Cuts at: 3028 3844 4638 4658 3028 Size: 816 794 20 4698 Fragments arranged by size: 4698 816 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6328 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6293 DraI TTT'AAA Cuts at: 3632 3632 Size: 6328 DraIII CAC_nnn'GTG Cuts at: 2175 2175 Size: 6328 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2443 5335 809 Size: 507 1127 2892 1802 Fragments arranged by size: 2892 1802 1127 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2236 2432 2654 Size: 54 1425 50 275 18 406 196 222 Cuts at: 2654 3160 3775 4664 6293 8 Size: 506 615 889 1629 43 Fragments arranged by size: 1629 1425 889 615 506 406 275 222 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2432 3775 1537 Size: 275 620 1343 4090 Fragments arranged by size: 4090 1343 620 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3402 4258 1353 Size: 2049 856 3423 Fragments arranged by size: 3423 2049 856 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3371 4915 5289 5435 387 Size: 1064 138 1782 1544 374 146 1280 Fragments arranged by size: 1782 1544 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2525 2685 2880 3426 3566 4391 Size: 393 614 279 160 195 546 140 825 Cuts at: 4391 4988 5775 6043 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 825 787 614 597 546 393 279 268 195 160 140 EcoO109I rG'GnC_Cy Cuts at: 2110 2119 2245 2265 2686 2687 2764 2822 3220 Size: 9 126 20 421 1 77 58 398 Cuts at: 3220 3265 2110 Size: 45 5173 Fragments arranged by size: 5173 421 398 126 77 58 45 20 9 1 EcoRI G'AATT_C Cuts at: 3011 3637 4015 3011 Size: 626 378 5324 Fragments arranged by size: 5324 626 378 FspI TGC'GCA Cuts at: 4834 4834 Size: 6328 HaeII r_GCGC'y Cuts at: 1678 3542 5475 1678 Size: 1864 1933 2531 Fragments arranged by size: 2531 1933 1864 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2352 2384 2658 2690 3199 Size: 32 135 32 780 32 274 32 509 Cuts at: 3199 3231 4969 5001 5043 5075 1373 Size: 32 1738 32 42 32 2626 Fragments arranged by size: 2626 1738 780 509 274 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2815 3672 3918 669 Size: 988 281 877 857 246 3079 Fragments arranged by size: 3079 988 877 857 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6328 HpaI GTT'AAC Cuts at: 3918 3918 Size: 6328 KpnI G_GTAC'C Cuts at: 3653 3653 Size: 6328 MfeI C'AATT_G Cuts at: 3927 3927 Size: 6328 MluI A'CGCG_T Cuts at: 6204 6204 Size: 6328 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2944 2976 3063 3351 5442 5626 1305 Size: 1639 32 87 288 2091 184 2007 Fragments arranged by size: 2091 2007 1639 288 184 87 32 MscI TGG'CCA Cuts at: 10 64 1489 1832 2238 3162 10 Size: 54 1425 343 406 924 3176 Fragments arranged by size: 3176 1425 924 406 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 2742 3134 3278 4286 4645 4804 Size: 648 1302 274 392 144 1008 359 159 Cuts at: 4804 518 Size: 2042 Fragments arranged by size: 2042 1302 1008 648 392 359 274 159 144 MspA1I CmG'CkG Cuts at: 1606 2488 3744 4421 5569 5814 1606 Size: 882 1256 677 1148 245 2120 Fragments arranged by size: 2120 1256 1148 882 677 245 NaeI GCC'GGC Cuts at: 2620 3515 2620 Size: 895 5433 NcoI C'CATG_G Cuts at: 513 1065 3129 513 Size: 552 2064 3712 Fragments arranged by size: 3712 2064 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6328 NgoMIV G'CCGG_C Cuts at: 2618 3513 2618 Size: 895 5433 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6328 NotI GC'GGCC_GC Cuts at: 3775 3775 Size: 6328 NspI r_CATG'y Cuts at: 1132 1229 3686 5231 1132 Size: 97 2457 1545 2229 Fragments arranged by size: 2457 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2320 2682 3336 1348 Size: 972 362 654 4340 Fragments arranged by size: 4340 972 654 362 PciI A'CATG_T Cuts at: 5227 5227 Size: 6328 PflMI CCAn_nnn'nTGG Cuts at: 1108 2663 1108 Size: 1555 4773 PfoI T'CCnGG_A Cuts at: 2662 2766 2883 2662 Size: 104 117 6107 Fragments arranged by size: 6107 117 104 PmeI GTTT'AAAC Cuts at: 3632 3632 Size: 6328 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4397 4429 5910 5942 4397 Size: 32 1481 32 4783 Fragments arranged by size: 4783 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2245 2265 2764 3265 2245 Size: 20 499 501 5308 Fragments arranged by size: 5308 501 499 20 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6328 PsiI TTA'TAA Cuts at: 3898 3898 Size: 6328 PspOMI G'GGCC_C Cuts at: 2686 2822 2686 Size: 136 6192 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2786 2818 2786 Size: 32 6296 PstI C_TGCA'G Cuts at: 830 3680 830 Size: 2850 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6328 PvuII CAG'CTG Cuts at: 2488 2488 Size: 6328 SalI G'TCGA_C Cuts at: 1936 3670 1936 Size: 1734 4594 SanDI GG'GwC_CC Cuts at: 2265 2265 Size: 6328 SbfI CC_TGCA'GG Cuts at: 3680 3680 Size: 6328 ScaI AGT'ACT Cuts at: 2909 4576 6288 2909 Size: 1667 1712 2949 Fragments arranged by size: 2949 1712 1667 SfcI C'TryA_G Cuts at: 826 1045 3676 4811 5492 5683 826 Size: 219 2631 1135 681 191 1471 Fragments arranged by size: 2631 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6328 SmaI CCC'GGG Cuts at: 1872 3655 1872 Size: 1783 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2131 2723 4432 5333 5595 820 Size: 197 926 188 592 1709 901 262 1553 Fragments arranged by size: 1709 1553 926 901 592 262 197 188 SnaBI TAC'GTA Cuts at: 493 3479 4119 493 Size: 2986 640 2702 Fragments arranged by size: 2986 2702 640 SpeI A'CTAG_T Cuts at: 152 152 Size: 6328 SphI G_CATG'C Cuts at: 1132 3686 1132 Size: 2554 3774 SspI AAT'ATT Cuts at: 5 52 4252 5 Size: 47 4200 2081 Fragments arranged by size: 4200 2081 47 StuI AGG'CCT Cuts at: 1517 3113 1517 Size: 1596 4732 StyI C'CwwG_G Cuts at: 513 1065 2818 3114 3129 3767 513 Size: 552 1753 296 15 638 3074 Fragments arranged by size: 3074 1753 638 552 296 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3122 4355 4372 4525 4710 5155 325 Size: 1255 1542 1233 17 153 185 445 1498 Fragments arranged by size: 1542 1498 1255 1233 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2793 2907 4574 Size: 274 80 33 51 1443 816 114 1667 Cuts at: 4574 6286 96 Size: 1712 138 Fragments arranged by size: 1712 1667 1443 816 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2441 2473 6288 6320 2441 Size: 32 3815 32 2449 Fragments arranged by size: 3815 2449 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6328 XbaI T'CTAG_A Cuts at: 3664 3664 Size: 6328 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6328 XmaI C'CCGG_G Cuts at: 1870 3653 1870 Size: 1783 4545 XmnI GAAnn'nnTTC Cuts at: 3500 4457 3500 Size: 957 5371 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4136 276 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI