Restriction map for
pFN21AE1351
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccI AclI AcyI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BclI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsiHKAI BsiWI BsmBI
BspDI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII CfoI
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KspI
MamI MfeI MluI MunI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 462 GGTACC G^GTACC
AccIII 575 TCCGGA T^CCGGA
AflII 858 CTTAAG C^TTAAG
AlwI 1579 GATCC GGATC(4/5)
AocI 1480 CCTGAGG CC^T[ACGT]AGG
Asp718I 462 GGTACC G^GTACC
AvaI 1393 CCCGGG C^[CT]CG[GA]G
BfrI 858 CTTAAG C^TTAAG
BglI 1135 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1274 CTCCAG CTGGAG(16/14)
BsaAI 1775 TACGTG [CT]AC^GT[GA]
BsaWI 575 TCCGGA [AT]^CCGG[AT]
BseAI 575 TCCGGA T^CCGGA
BsmI 507 GAATGC GAATGC(1/-1)
BsoBI 1393 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1439 TGTACA T^GTACA
BspEI 575 TCCGGA T^CCGGA
BspMI 171 ACCTGC ACCTGC(4/8)
BsrBI 1890 CCGCTC CCGCTC(-3/-3)
BsrGI 1439 TGTACA T^GTACA
BstYI 1578 AGATCC [GA]^GATC[CT]
Bsu36I 1480 CCTGAGG CC^T[ACGT]AGG
DrdI 1288 GACATCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1480 CCTGAGG CC^T[ACGT]AGG
EcoNI 375 CCTTAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 253 AAGCTT A^AGCTT
KpnI 462 GGTACC GGTAC^C
MflI 1578 AGATCC [GA]^GATC[CT]
MroI 575 TCCGGA T^CCGGA
MslI 1791 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 45 CAGCGG C[AC]G^C[GT]G
NaeI 1654 GCCGGC GCC^GGC
NcoI 262 CCATGG C^CATGG
NdeI 968 CATATG CA^TATG
NgoMIV 1654 GCCGGC G^CCGGC
NspI 125 GCATGC [GA]CATG^[CT]
PshAI 1239 GACCGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 922 CTGCAG CTGCA^G
ScaI 1638 AGTACT AGT^ACT
SmaI 1393 CCCGGG CCC^GGG
SphI 125 GCATGC GCATG^C
SspBI 1439 TGTACA T^GTACA
XcmI 420 CCACATCCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1578 AGATCC [GA]^GATC[CT]
XmaCI 1393 CCCGGG C^CCGGG
XmaI 1393 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 37 CGCG CG^CG
143 CGCG
AcsI 333 GAATTT [GA]^AATT[CT]
1615 GAATTT
ApoI 333 GAATTT [GA]^AATT[CT]
1615 GAATTT
Asp700I 251 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
822 GAAGGAGTTC
BciVI 1062 GGATAC GTATCC(6/5)
1697 GTATCC
BsiEI 14 CGGTCG CG[GA][CT]^CG
1146 CGACCG
BsrFI 1148 ACCGGC [GA]^CCGG[CT]
1654 GCCGGC
BstUI 37 CGCG CG^CG
143 CGCG
Cfr10I 1148 ACCGGC [GA]^CCGG[CT]
1654 GCCGGC
DsaI 262 CCATGG C^C[GA][CT]GG
1190 CCGTGG
HgaI 1197 GACGC GACGC(5/10)
65 GCGTC
MaeII 553 ACGT A^CGT
1776 ACGT
MvnI 37 CGCG CG^CG
143 CGCG
PflMI 446 CCATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
843 CCAATACCTGG
PleI 277 GACTC GAGTC(4/5)
1710 GACTC
SmlI 772 CTCAAG C^T[CT][GA]AG
858 CTTAAG
Van91I 446 CCATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
843 CCAATACCTGG
XmnI 251 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
822 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI